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Identification of iridoid glucoside transporters in Catharanthus roseus

(2017) PLANT AND CELL PHYSIOLOGY. 58(9). p.1507-1518
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Abstract
Monoterpenoid indole alkaloids (MIAs) are plant defense compounds and high-value pharmaceuticals. Biosynthesis of the universal MIA precursor, secologanin, is organized between internal phloem-associated parenchyma (IPAP) and epidermis cells. Transporters for intercellular transport of proposed mobile pathway intermediates have remained elusive. Screening of an Arabidopsis thaliana transporter library expressed in Xenopus oocytes identified AtNPF2.9 as a putative iridoid glucoside importer. Eight orthologs were identified in Catharanthus roseus, of which three, CrNPF2.4, CrNPF2.5 and CrNPF2.6, were capable of transporting the iridoid glucosides 7-deoxyloganic acid, loganic acid, loganin and secologanin into oocytes. Based on enzyme expression data and transporter specificity, we propose that several enzymes of the biosynthetic pathway are present in both IPAP and epidermis cells, and that the three transporters are responsible for transporting not only loganic acid, as previously proposed, but multiple intermediates. Identification of the iridoid glucoside-transporting CrNPFs is an important step toward understanding the complex orchestration of the seco-iridioid pathway.
Keywords
INDOLE ALKALOID PATHWAY, ACID CARBOXYL METHYLTRANSFERASE, BINDING, CASSETTE PROTEIN, ADENOSYL-L-METHIONINE, SUBCELLULAR ORGANIZATION, MADAGASCAR PERIWINKLE, VACUOLAR TRANSPORT, COPTIS-JAPONICA, PLANT, BIOSYNTHESIS, Catharanthus roseus, Iridoid glucosides, Mobile intermediates, Monoterpenoid indole alkaloids, NPF transporters, Pathway orchestration

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Chicago
Larsen, Bo, Victoria L Fuller, Jacob Pollier, Alex Van Moerkercke, Fabian Schweizer, Richard Payne, Maite Colinas Martinez, Sarah E O’Connor, Alain Goossens, and Barbara A Halkier. 2017. “Identification of Iridoid Glucoside Transporters in Catharanthus Roseus.” Plant and Cell Physiology 58 (9): 1507–1518.
APA
Larsen, B., Fuller, V. L., Pollier, J., Van Moerkercke, A., Schweizer, F., Payne, R., Colinas Martinez, M., et al. (2017). Identification of iridoid glucoside transporters in Catharanthus roseus. PLANT AND CELL PHYSIOLOGY, 58(9), 1507–1518.
Vancouver
1.
Larsen B, Fuller VL, Pollier J, Van Moerkercke A, Schweizer F, Payne R, et al. Identification of iridoid glucoside transporters in Catharanthus roseus. PLANT AND CELL PHYSIOLOGY. 2017;58(9):1507–18.
MLA
Larsen, Bo, Victoria L Fuller, Jacob Pollier, et al. “Identification of Iridoid Glucoside Transporters in Catharanthus Roseus.” PLANT AND CELL PHYSIOLOGY 58.9 (2017): 1507–1518. Print.
@article{8533308,
  abstract     = {Monoterpenoid indole alkaloids (MIAs) are plant defense compounds and high-value pharmaceuticals. Biosynthesis of the universal MIA precursor, secologanin, is organized between internal phloem-associated parenchyma (IPAP) and epidermis cells. Transporters for intercellular transport of proposed mobile pathway intermediates have remained elusive. Screening of an Arabidopsis thaliana transporter library expressed in Xenopus oocytes identified AtNPF2.9 as a putative iridoid glucoside importer. Eight orthologs were identified in Catharanthus roseus, of which three, CrNPF2.4, CrNPF2.5 and CrNPF2.6, were capable of transporting the iridoid glucosides 7-deoxyloganic acid, loganic acid, loganin and secologanin into oocytes. Based on enzyme expression data and transporter specificity, we propose that several enzymes of the biosynthetic pathway are present in both IPAP and epidermis cells, and that the three transporters are responsible for transporting not only loganic acid, as previously proposed, but multiple intermediates. Identification of the iridoid glucoside-transporting CrNPFs is an important step toward understanding the complex orchestration of the seco-iridioid pathway.},
  author       = {Larsen, Bo and Fuller, Victoria L and Pollier, Jacob and Van Moerkercke, Alex and Schweizer, Fabian and Payne, Richard and Colinas Martinez, Maite and O'Connor, Sarah E and Goossens, Alain and Halkier, Barbara A},
  issn         = {0032-0781},
  journal      = {PLANT AND CELL PHYSIOLOGY},
  keyword      = {INDOLE ALKALOID PATHWAY,ACID CARBOXYL METHYLTRANSFERASE,BINDING,CASSETTE PROTEIN,ADENOSYL-L-METHIONINE,SUBCELLULAR ORGANIZATION,MADAGASCAR PERIWINKLE,VACUOLAR TRANSPORT,COPTIS-JAPONICA,PLANT,BIOSYNTHESIS,Catharanthus roseus,Iridoid glucosides,Mobile intermediates,Monoterpenoid indole alkaloids,NPF transporters,Pathway orchestration},
  language     = {eng},
  number       = {9},
  pages        = {1507--1518},
  title        = {Identification of iridoid glucoside transporters in Catharanthus roseus},
  url          = {http://dx.doi.org/10.1093/pcp/pcx097},
  volume       = {58},
  year         = {2017},
}

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