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Validation and cost-effectiveness of an alternative method to quantify Batrachochytrium dendrobatidis infection in amphibian samples using real-time PCR

Stefano Canessa UGent, Marco Maggesi, Sebastiano Salvidio and Elena Grasselli (2017) RENDICONTI LINCEI-SCIENZE FISICHE E NATURALI. 28(4). p.687-692
abstract
The chytrid fungus Batrachochytrium dendrobatidis (Bd) is considered one of the main causes of amphibian declines worldwide. Detection of Bd infection relies on both histological and molecular techniques, but only quantitative real-time PCR (qPCR) provides quantitative information on intensity of infection. To quantify Bd infection, Boyle et al. (2004) developed a standard method using TaqMan qPCR assay: however, the high costs of this method can hinder its applicability for studies with limited resources. Starting from the method by Boyle et al. (2004), we set up and fully validated a qPCR assay based on modified primers and SYBR Green chemistry. The modified SYBR Green has high sensitivity and specificity, as well as the ability to quantify Bd infection loads as well as the standard TaqMan qPCR, but it is more than 60% cheaper. This is obtained both by intrinsic cost-effectiveness of the SYBR Green technique and by reducing reaction volumes of qPCR. Therefore, this modified qPCR assay constitutes a cheaper molecular method to quantify Bd in amphibians.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Chytridiomycosis, Amphibian disease, Infection loads, Monitoring, qPCR, Cost evaluation, CHYTRID FUNGUS, SP-NOV, CHYTRIDIOMYCOSIS, POPULATIONS, DISEASE, ASSAY
journal title
RENDICONTI LINCEI-SCIENZE FISICHE E NATURALI
Rend. Lincei.-Sci. Fis. Nat.
volume
28
issue
4
pages
687 - 692
Web of Science type
Article
Web of Science id
000414721600011
ISSN
2037-4631
1720-0776
DOI
10.1007/s12210-017-0640-0
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8532593
handle
http://hdl.handle.net/1854/LU-8532593
date created
2017-09-28 11:22:20
date last changed
2018-06-05 08:37:13
@article{8532593,
  abstract     = {The chytrid fungus Batrachochytrium dendrobatidis (Bd) is considered one of the main causes of amphibian declines worldwide. Detection of Bd infection relies on both histological and molecular techniques, but only quantitative real-time PCR (qPCR) provides quantitative information on intensity of infection. To quantify Bd infection, Boyle et al. (2004) developed a standard method using TaqMan qPCR assay: however, the high costs of this method can hinder its applicability for studies with limited resources. Starting from the method by Boyle et al. (2004), we set up and fully validated a qPCR assay based on modified primers and SYBR Green chemistry. The modified SYBR Green has high sensitivity and specificity, as well as the ability to quantify Bd infection loads as well as the standard TaqMan qPCR, but it is more than 60\% cheaper. This is obtained both by intrinsic cost-effectiveness of the SYBR Green technique and by reducing reaction volumes of qPCR. Therefore, this modified qPCR assay constitutes a cheaper molecular method to quantify Bd in amphibians.},
  author       = {Canessa, Stefano and Maggesi, Marco and Salvidio, Sebastiano and Grasselli, Elena},
  issn         = {2037-4631},
  journal      = {RENDICONTI LINCEI-SCIENZE FISICHE E NATURALI},
  keyword      = {Chytridiomycosis,Amphibian disease,Infection loads,Monitoring,qPCR,Cost evaluation,CHYTRID FUNGUS,SP-NOV,CHYTRIDIOMYCOSIS,POPULATIONS,DISEASE,ASSAY},
  language     = {eng},
  number       = {4},
  pages        = {687--692},
  title        = {Validation and cost-effectiveness of an alternative method to quantify Batrachochytrium dendrobatidis infection in amphibian samples using real-time PCR},
  url          = {http://dx.doi.org/10.1007/s12210-017-0640-0},
  volume       = {28},
  year         = {2017},
}

Chicago
Canessa, Stefano, Marco Maggesi, Sebastiano Salvidio, and Elena Grasselli. 2017. “Validation and Cost-effectiveness of an Alternative Method to Quantify Batrachochytrium Dendrobatidis Infection in Amphibian Samples Using Real-time PCR.” Rendiconti Lincei-scienze Fisiche E Naturali 28 (4): 687–692.
APA
Canessa, S., Maggesi, M., Salvidio, S., & Grasselli, E. (2017). Validation and cost-effectiveness of an alternative method to quantify Batrachochytrium dendrobatidis infection in amphibian samples using real-time PCR. RENDICONTI LINCEI-SCIENZE FISICHE E NATURALI, 28(4), 687–692.
Vancouver
1.
Canessa S, Maggesi M, Salvidio S, Grasselli E. Validation and cost-effectiveness of an alternative method to quantify Batrachochytrium dendrobatidis infection in amphibian samples using real-time PCR. RENDICONTI LINCEI-SCIENZE FISICHE E NATURALI. 2017;28(4):687–92.
MLA
Canessa, Stefano, Marco Maggesi, Sebastiano Salvidio, et al. “Validation and Cost-effectiveness of an Alternative Method to Quantify Batrachochytrium Dendrobatidis Infection in Amphibian Samples Using Real-time PCR.” RENDICONTI LINCEI-SCIENZE FISICHE E NATURALI 28.4 (2017): 687–692. Print.