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Community composition determines activity of antibiotics against multispecies biofilms

Sarah Tavernier (UGent) , Aurélie Crabbé (UGent) , Mayram Hacioglu, Liesbeth Stuer, Silke Henry (UGent) , Petra Rigole (UGent) , Inne Dhondt (UGent) and Tom Coenye (UGent)
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Abstract
In young cystic fibrosis (CF) patients, Staphylococcus aureus is typically the most prevalent organism, while in adults, Pseudomonas aeruginosa is the major pathogen. More recently, it was observed that also Streptococcus anginosus plays an important role in exacerbations of respiratory symptoms. These species are often coisolated from CF lungs, yet little is known about whether antibiotic killing of one species is influenced by the presence of others. In the present study, we compared the activities of various antibiotics against S. anginosus, S. aureus, and P. aeruginosa when grown in monospecies biofilms with the activity observed in a multispecies biofilm. Our results show that differences in antibiotic activity against species grown in mono- and multispecies biofilms are species and antibiotic dependent. Fewer S. anginosus cells are killed by antibiotics that interfere with cell wall synthesis (amoxicillin plus sulbactam, cefepime, imipenem, meropenem, and vancomycin) in the presence of S. aureus and P. aeruginosa, while for ciprofloxacin, levofloxacin, and tobramycin, no difference was observed. In addition, we observed that the cell-free supernatant of S. aureus, but not that of P. aeruginosa biofilms, also caused this decrease in killing. Overall, S. aureus was more affected by antibiotic treatment in a multispecies biofilm, while for P. aeruginosa, no differences were observed between growth in mono- or multispecies biofilms. The results of the present study suggest that it is important to take the community composition into account when evaluating the effect of antimicrobial treatments against certain species in mixed biofilms.
Keywords
multispecies biofilm, Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus anginosus, CYSTIC-FIBROSIS PATIENTS, PSEUDOMONAS-AERUGINOSA BIOFILMS, STREPTOCOCCUS-MILLERI GROUP, STAPHYLOCOCCUS-AUREUS, BURKHOLDERIA-CEPACIA, PHARMACOKINETICS, INFECTIONS, INHALATION, SAFETY, DIVERSITY

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Chicago
Tavernier, Sarah, Aurélie Crabbé, Mayram Hacioglu, Liesbeth Stuer, Silke Henry, Petra Rigole, Inne Dhondt, and Tom Coenye. 2017. “Community Composition Determines Activity of Antibiotics Against Multispecies Biofilms.” Antimicrobial Agents and Chemotherapy 61 (9).
APA
Tavernier, Sarah, Crabbé, A., Hacioglu, M., Stuer, L., Henry, S., Rigole, P., Dhondt, I., et al. (2017). Community composition determines activity of antibiotics against multispecies biofilms. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 61(9).
Vancouver
1.
Tavernier S, Crabbé A, Hacioglu M, Stuer L, Henry S, Rigole P, et al. Community composition determines activity of antibiotics against multispecies biofilms. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY. 2017;61(9).
MLA
Tavernier, Sarah, Aurélie Crabbé, Mayram Hacioglu, et al. “Community Composition Determines Activity of Antibiotics Against Multispecies Biofilms.” ANTIMICROBIAL AGENTS AND CHEMOTHERAPY 61.9 (2017): n. pag. Print.
@article{8532519,
  abstract     = {In young cystic fibrosis (CF) patients, Staphylococcus aureus is typically the most prevalent organism, while in adults, Pseudomonas aeruginosa is the major pathogen. More recently, it was observed that also Streptococcus anginosus plays an important role in exacerbations of respiratory symptoms. These species are often coisolated from CF lungs, yet little is known about whether antibiotic killing of one species is influenced by the presence of others. In the present study, we compared the activities of various antibiotics against S. anginosus, S. aureus, and P. aeruginosa when grown in monospecies biofilms with the activity observed in a multispecies biofilm. Our results show that differences in antibiotic activity against species grown in mono- and multispecies biofilms are species and antibiotic dependent. Fewer S. anginosus cells are killed by antibiotics that interfere with cell wall synthesis (amoxicillin plus sulbactam, cefepime, imipenem, meropenem, and vancomycin) in the presence of S. aureus and P. aeruginosa, while for ciprofloxacin, levofloxacin, and tobramycin, no difference was observed. In addition, we observed that the cell-free supernatant of S. aureus, but not that of P. aeruginosa biofilms, also caused this decrease in killing. Overall, S. aureus was more affected by antibiotic treatment in a multispecies biofilm, while for P. aeruginosa, no differences were observed between growth in mono- or multispecies biofilms. The results of the present study suggest that it is important to take the community composition into account when evaluating the effect of antimicrobial treatments against certain species in mixed biofilms.},
  articleno    = {e00302-17},
  author       = {Tavernier, Sarah and Crabb{\'e}, Aur{\'e}lie and Hacioglu, Mayram and Stuer, Liesbeth and Henry, Silke and Rigole, Petra and Dhondt, Inne and Coenye, Tom},
  issn         = {0066-4804},
  journal      = {ANTIMICROBIAL AGENTS AND CHEMOTHERAPY},
  keyword      = {multispecies biofilm,Pseudomonas aeruginosa,Staphylococcus aureus,Streptococcus anginosus,CYSTIC-FIBROSIS PATIENTS,PSEUDOMONAS-AERUGINOSA BIOFILMS,STREPTOCOCCUS-MILLERI GROUP,STAPHYLOCOCCUS-AUREUS,BURKHOLDERIA-CEPACIA,PHARMACOKINETICS,INFECTIONS,INHALATION,SAFETY,DIVERSITY},
  language     = {eng},
  number       = {9},
  pages        = {12},
  title        = {Community composition determines activity of antibiotics against multispecies biofilms},
  url          = {http://dx.doi.org/10.1128/aac.00302-17},
  volume       = {61},
  year         = {2017},
}

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