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Laccase enzyme detoxifies hydrolysates and improves biogas production from hemp straw and miscanthus

Michel Schroyen (UGent) , Stijn Van Hulle (UGent) , Sander Holemans, Han Vervaeren (UGent) and Katleen Raes (UGent)
(2017) BIORESOURCE TECHNOLOGY. 244(1). p.597-604
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Abstract
The impact of various phenolic compounds, vanillic acid, ferulic acid, p-coumaric acid and 4-hydroxybenzoic acid on anaerobic digestion of lignocellulosic biomass (hemp straw and miscanthus) was studied. Such phenolic compounds have been known to inhibit biogas production during anaerobic digestion. The different phenolic compounds were added in various concentrations: 0, 100, 500, 1000 and 2000 mg/L. A difference in inhibition of biomethane production between the phenolic compounds was noted. Hydrolysis rate, during anaerobic digestion of miscanthus was inhibited up to 50% by vanillic acid, while vanillic acid had no influence on the initial rate of biogas production during the anaerobic digestion of hemp straw. Miscanthus has a higher lignin concentration (12-30 g/100 g DM) making it less accessible for degradation, and in combination with phenolic compounds released after harsh pretreatments, it can cause severe inhibition levels during the anaerobic digestion, lowering biogas production. To counter the inhibition, lignin degrading enzymes can be used to remove or degrade the inhibitory phenolic compounds. The interaction of laccase and versatile peroxidase individually with the different phenolic compounds was studied to have insight in the polymerization of inhibitory compounds or breakdown of lignocellulose. Hemp straw and miscanthus were incubated with 0, 100 and 500 mg/L of the different phenolic compounds for 0, 6 and 24 h and pretreated with the lignin degrading enzymes. A laccase pretreatment successfully detoxified the substrate, while versatile peroxidase however was inhibited by 100 mg/L of each of the individual phenolic compounds. Finally a combination of enzymatic detoxification and subsequent biogas production showed that a decrease in phenolic compounds by laccase treatment can considerably lower the inhibition levels of the biogas production.
Keywords
Phenolic compounds, Inhibition, Laccase, Versatile peroxidase, Anaerobic digestion, Lignocellulose, PHENOLIC-COMPOUNDS, LIGNOCELLULOSIC BIOMASS, VERSATILE PEROXIDASE, SUGARCANE BAGASSE, STEAM EXPLOSION, CORN STOVER, PRETREATMENT, LIGNIN, BIODEGRADABILITY, FUNDAMENTALS

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MLA
Schroyen, Michel et al. “Laccase Enzyme Detoxifies Hydrolysates and Improves Biogas Production from Hemp Straw and Miscanthus.” BIORESOURCE TECHNOLOGY 244.1 (2017): 597–604. Print.
APA
Schroyen, Michel, Van Hulle, S., Holemans, S., Vervaeren, H., & Raes, K. (2017). Laccase enzyme detoxifies hydrolysates and improves biogas production from hemp straw and miscanthus. BIORESOURCE TECHNOLOGY, 244(1), 597–604.
Chicago author-date
Schroyen, Michel, Stijn Van Hulle, Sander Holemans, Han Vervaeren, and Katleen Raes. 2017. “Laccase Enzyme Detoxifies Hydrolysates and Improves Biogas Production from Hemp Straw and Miscanthus.” Bioresource Technology 244 (1): 597–604.
Chicago author-date (all authors)
Schroyen, Michel, Stijn Van Hulle, Sander Holemans, Han Vervaeren, and Katleen Raes. 2017. “Laccase Enzyme Detoxifies Hydrolysates and Improves Biogas Production from Hemp Straw and Miscanthus.” Bioresource Technology 244 (1): 597–604.
Vancouver
1.
Schroyen M, Van Hulle S, Holemans S, Vervaeren H, Raes K. Laccase enzyme detoxifies hydrolysates and improves biogas production from hemp straw and miscanthus. BIORESOURCE TECHNOLOGY. 2017;244(1):597–604.
IEEE
[1]
M. Schroyen, S. Van Hulle, S. Holemans, H. Vervaeren, and K. Raes, “Laccase enzyme detoxifies hydrolysates and improves biogas production from hemp straw and miscanthus,” BIORESOURCE TECHNOLOGY, vol. 244, no. 1, pp. 597–604, 2017.
@article{8532474,
  abstract     = {The impact of various phenolic compounds, vanillic acid, ferulic acid, p-coumaric acid and 4-hydroxybenzoic acid on anaerobic digestion of lignocellulosic biomass (hemp straw and miscanthus) was studied. Such phenolic compounds have been known to inhibit biogas production during anaerobic digestion. The different phenolic compounds were added in various concentrations: 0, 100, 500, 1000 and 2000 mg/L. A difference in inhibition of biomethane production between the phenolic compounds was noted. Hydrolysis rate, during anaerobic digestion of miscanthus was inhibited up to 50% by vanillic acid, while vanillic acid had no influence on the initial rate of biogas production during the anaerobic digestion of hemp straw. Miscanthus has a higher lignin concentration (12-30 g/100 g DM) making it less accessible for degradation, and in combination with phenolic compounds released after harsh pretreatments, it can cause severe inhibition levels during the anaerobic digestion, lowering biogas production. To counter the inhibition, lignin degrading enzymes can be used to remove or degrade the inhibitory phenolic compounds. The interaction of laccase and versatile peroxidase individually with the different phenolic compounds was studied to have insight in the polymerization of inhibitory compounds or breakdown of lignocellulose. Hemp straw and miscanthus were incubated with 0, 100 and 500 mg/L of the different phenolic compounds for 0, 6 and 24 h and pretreated with the lignin degrading enzymes. A laccase pretreatment successfully detoxified the substrate, while versatile peroxidase however was inhibited by 100 mg/L of each of the individual phenolic compounds. Finally a combination of enzymatic detoxification and subsequent biogas production showed that a decrease in phenolic compounds by laccase treatment can considerably lower the inhibition levels of the biogas production.},
  author       = {Schroyen, Michel and Van Hulle, Stijn and Holemans, Sander and Vervaeren, Han and Raes, Katleen},
  issn         = {0960-8524},
  journal      = {BIORESOURCE TECHNOLOGY},
  keywords     = {Phenolic compounds,Inhibition,Laccase,Versatile peroxidase,Anaerobic digestion,Lignocellulose,PHENOLIC-COMPOUNDS,LIGNOCELLULOSIC BIOMASS,VERSATILE PEROXIDASE,SUGARCANE BAGASSE,STEAM EXPLOSION,CORN STOVER,PRETREATMENT,LIGNIN,BIODEGRADABILITY,FUNDAMENTALS},
  language     = {eng},
  number       = {1},
  pages        = {597--604},
  title        = {Laccase enzyme detoxifies hydrolysates and improves biogas production from hemp straw and miscanthus},
  url          = {http://dx.doi.org/10.1016/j.biortech.2017.07.137},
  volume       = {244},
  year         = {2017},
}

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