Advanced search
1 file | 1.74 MB

Staphylococcal serine protease-like proteins are pacemakers of allergic airway reactions to Staphylococcus aureus

Author
Organization
Abstract
Background: A substantial subgroup of asthmatic patients have "nonallergic'' or idiopathic asthma, which often takes a severe course and is difficult to treat. The cause might be allergic reactions to the gram-positive pathogen Staphylococcus aureus, a frequent colonizer of the upper airways. However, the driving allergens of S aureus have remained elusive. Objective: We sought to search for potentially allergenic S aureus proteins and characterize the immune response directed against them. Methods: S aureus extracellular proteins targeted by human serum IgG4 were identified by means of immunoblotting to screen for potential bacterial allergens. Candidate antigens were expressed as recombinant proteins and used to analyze the established cellular and humoral immune responses in healthy adults and asthmatic patients. The ability to induce a type 2 immune response in vivo was tested in a mouse asthma model. Results: We identified staphylococcal serine protease-like proteins (Spls) as dominant IgG4-binding S aureus proteins. SplA through SplF are extracellular proteases of unknown function expressed by S aureus in vivo. Spls elicited IgE antibody responses in most asthmatic patients. In healthy S aureus carriers and noncarriers, peripheral blood T cells elaborated TH2 cytokines after stimulation with Spls, as is typical for allergens. In contrast, TH1/TH17 cytokines, which dominated the response to S aureusahemolysin, were of low concentration or absent. In mice inhalation of SplD without adjuvant induced lung inflammation characterized by TH2 cytokines and eosinophil infiltration. Conclusion: We identify Spls as triggering allergens released by S aureus, opening prospects for diagnosis and causal therapy of asthma.
Keywords
Asthma, IgE, Staphylococcus aureus, serine proteaselike proteins, type 2 inflammation, HYPER-IGE SYNDROME, B-CELL MEMORY, BACILLUS-SUBTILIS, CHRONIC RHINOSINUSITIS, IMMUNE-RESPONSE, ENTEROTOXIN-B, MAST-CELLS, ASTHMA, SUPERANTIGENS, DISEASE

Downloads

  • stenzel et al 2017.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 1.74 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Stentzel, Sebastian, Andrea Renate Teufelberger, Maria Nordengrün, Julia Kolata, Frank Schmidt, Koen Van Crombruggen, Stephan Michalik, et al. 2017. “Staphylococcal Serine Protease-like Proteins Are Pacemakers of Allergic Airway Reactions to Staphylococcus Aureus.” Journal of Allergy and Clinical Immunology 139 (2): 492–500.
APA
Stentzel, S., Teufelberger, A. R., Nordengrün, M., Kolata, J., Schmidt, F., Van Crombruggen, K., Michalik, S., et al. (2017). Staphylococcal serine protease-like proteins are pacemakers of allergic airway reactions to Staphylococcus aureus. JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 139(2), 492–500.
Vancouver
1.
Stentzel S, Teufelberger AR, Nordengrün M, Kolata J, Schmidt F, Van Crombruggen K, et al. Staphylococcal serine protease-like proteins are pacemakers of allergic airway reactions to Staphylococcus aureus. JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY. 2017;139(2):492–500.
MLA
Stentzel, Sebastian, Andrea Renate Teufelberger, Maria Nordengrün, et al. “Staphylococcal Serine Protease-like Proteins Are Pacemakers of Allergic Airway Reactions to Staphylococcus Aureus.” JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY 139.2 (2017): 492–500. Print.
@article{8532402,
  abstract     = {Background: A substantial subgroup of asthmatic patients have {\textacutedbl}nonallergic'' or idiopathic asthma, which often takes a severe course and is difficult to treat. The cause might be allergic reactions to the gram-positive pathogen Staphylococcus aureus, a frequent colonizer of the upper airways. However, the driving allergens of S aureus have remained elusive. 
Objective: We sought to search for potentially allergenic S aureus proteins and characterize the immune response directed against them. 
Methods: S aureus extracellular proteins targeted by human serum IgG4 were identified by means of immunoblotting to screen for potential bacterial allergens. Candidate antigens were expressed as recombinant proteins and used to analyze the established cellular and humoral immune responses in healthy adults and asthmatic patients. The ability to induce a type 2 immune response in vivo was tested in a mouse asthma model. 
Results: We identified staphylococcal serine protease-like proteins (Spls) as dominant IgG4-binding S aureus proteins. SplA through SplF are extracellular proteases of unknown function expressed by S aureus in vivo. Spls elicited IgE antibody responses in most asthmatic patients. In healthy S aureus carriers and noncarriers, peripheral blood T cells elaborated TH2 cytokines after stimulation with Spls, as is typical for allergens. In contrast, TH1/TH17 cytokines, which dominated the response to S aureusahemolysin, were of low concentration or absent. In mice inhalation of SplD without adjuvant induced lung inflammation characterized by TH2 cytokines and eosinophil infiltration. 
Conclusion: We identify Spls as triggering allergens released by S aureus, opening prospects for diagnosis and causal therapy of asthma.},
  author       = {Stentzel, Sebastian and Teufelberger, Andrea Renate and Nordengr{\"u}n, Maria and Kolata, Julia and Schmidt, Frank and Van Crombruggen, Koen and Michalik, Stephan and Kumpfm{\"u}ller, Jana and Tischer, Sebastian and Schweder, Thomas and Hecker, Michael and Engelmann, Susanne and V{\"o}lker, Uwe and Krysko, Olga and Bachert, Claus and Br{\"o}ker, Barbara M},
  issn         = {0091-6749},
  journal      = {JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY},
  keyword      = {Asthma,IgE,Staphylococcus aureus,serine proteaselike proteins,type 2 inflammation,HYPER-IGE SYNDROME,B-CELL MEMORY,BACILLUS-SUBTILIS,CHRONIC RHINOSINUSITIS,IMMUNE-RESPONSE,ENTEROTOXIN-B,MAST-CELLS,ASTHMA,SUPERANTIGENS,DISEASE},
  language     = {eng},
  number       = {2},
  pages        = {492--500},
  title        = {Staphylococcal serine protease-like proteins are pacemakers of allergic airway reactions to Staphylococcus aureus},
  url          = {http://dx.doi.org/10.1016/j.jaci.2016.03.045},
  volume       = {139},
  year         = {2017},
}

Altmetric
View in Altmetric
Web of Science
Times cited: