Advanced search
1 file | 739.26 KB

Analysis of iodinated quorum sensing peptides by LC-UV/ESI ion trap mass spectrometry

Author
Organization
Abstract
Five different quorum sensing peptides (QSP) were iodinated using different iodination techniques. These iodinated peptides were analyzed using a C-18 reversed phase HPLC system, applying a linear gradient of water and acetonitrile containing 0.1% (m/v) formic acid as mobile phase. Electrospray ionization (ESI) ion trap mass spectrometry was used for the identification of the modified peptides, while semi-quantification was performed using total ion current (TIC) spectra. Non-iodinated peptides and mono- and di-iodinated peptides (NIP, MIP and DIP respectively) were well separated and eluted in that order. Depending on the used iodination method, iodination yields varied from low (2%) to high (57%).
Keywords
Peptide iodination, LC-UV/MS, Quorum sensing, Bolton-Hunter, PERFORMANCE LIQUID-CHROMATOGRAPHY, BLOOD-BRAIN-BARRIER, ENTEROCOCCUS-FAECALIS, CHEMICAL SPACE, PROTEINS, PENTAPEPTIDE, BACTERIA, CANCER, I-125

Downloads

  • 8587814.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 739.26 KB

Citation

Please use this url to cite or link to this publication:

Chicago
Janssens, Yorick, Frederick Verbeke, Nathan Debunne, Evelien Wynendaele, Kathelijne Peremans, and Bart De Spiegeleer. 2018. “Analysis of Iodinated Quorum Sensing Peptides by LC-UV/ESI Ion Trap Mass Spectrometry.” Journal of Pharmaceutical Analysis 8 (1): 69–74.
APA
Janssens, Yorick, Verbeke, F., Debunne, N., Wynendaele, E., Peremans, K., & De Spiegeleer, B. (2018). Analysis of iodinated quorum sensing peptides by LC-UV/ESI ion trap mass spectrometry. JOURNAL OF PHARMACEUTICAL ANALYSIS, 8(1), 69–74.
Vancouver
1.
Janssens Y, Verbeke F, Debunne N, Wynendaele E, Peremans K, De Spiegeleer B. Analysis of iodinated quorum sensing peptides by LC-UV/ESI ion trap mass spectrometry. JOURNAL OF PHARMACEUTICAL ANALYSIS. 2018;8(1):69–74.
MLA
Janssens, Yorick et al. “Analysis of Iodinated Quorum Sensing Peptides by LC-UV/ESI Ion Trap Mass Spectrometry.” JOURNAL OF PHARMACEUTICAL ANALYSIS 8.1 (2018): 69–74. Print.
@article{8531998,
  abstract     = {Five different quorum sensing peptides (QSP) were iodinated using different iodination techniques. These iodinated peptides were analyzed using a C-18 reversed phase HPLC system, applying a linear gradient of water and acetonitrile containing 0.1% (m/v) formic acid as mobile phase. Electrospray ionization (ESI) ion trap mass spectrometry was used for the identification of the modified peptides, while semi-quantification was performed using total ion current (TIC) spectra. Non-iodinated peptides and mono- and di-iodinated peptides (NIP, MIP and DIP respectively) were well separated and eluted in that order. Depending on the used iodination method, iodination yields varied from low (2%) to high (57%).},
  author       = {Janssens, Yorick and Verbeke, Frederick and Debunne, Nathan and Wynendaele, Evelien and Peremans, Kathelijne and De Spiegeleer, Bart},
  issn         = {2095-1779},
  journal      = {JOURNAL OF PHARMACEUTICAL ANALYSIS},
  keywords     = {Peptide iodination,LC-UV/MS,Quorum sensing,Bolton-Hunter,PERFORMANCE LIQUID-CHROMATOGRAPHY,BLOOD-BRAIN-BARRIER,ENTEROCOCCUS-FAECALIS,CHEMICAL SPACE,PROTEINS,PENTAPEPTIDE,BACTERIA,CANCER,I-125},
  language     = {eng},
  number       = {1},
  pages        = {69--74},
  title        = {Analysis of iodinated quorum sensing peptides by LC-UV/ESI ion trap mass spectrometry},
  url          = {http://dx.doi.org/10.1016/j.jpha.2017.09.001},
  volume       = {8},
  year         = {2018},
}

Altmetric
View in Altmetric
Web of Science
Times cited: