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Analysis of iodinated quorum sensing peptides by LC-UV/ESI ion trap mass spectrometry

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Abstract
Five different quorum sensing peptides (QSP) were iodinated using different iodination techniques. These iodinated peptides were analyzed using a C-18 reversed phase HPLC system, applying a linear gradient of water and acetonitrile containing 0.1% (m/v) formic acid as mobile phase. Electrospray ionization (ESI) ion trap mass spectrometry was used for the identification of the modified peptides, while semi-quantification was performed using total ion current (TIC) spectra. Non-iodinated peptides and mono- and di-iodinated peptides (NIP, MIP and DIP respectively) were well separated and eluted in that order. Depending on the used iodination method, iodination yields varied from low (2%) to high (57%).
Keywords
Peptide iodination, LC-UV/MS, Quorum sensing, Bolton-Hunter, PERFORMANCE LIQUID-CHROMATOGRAPHY, BLOOD-BRAIN-BARRIER, ENTEROCOCCUS-FAECALIS, CHEMICAL SPACE, PROTEINS, PENTAPEPTIDE, BACTERIA, CANCER, I-125

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MLA
Janssens, Yorick, et al. “Analysis of Iodinated Quorum Sensing Peptides by LC-UV/ESI Ion Trap Mass Spectrometry.” JOURNAL OF PHARMACEUTICAL ANALYSIS, vol. 8, no. 1, 2018, pp. 69–74, doi:10.1016/j.jpha.2017.09.001.
APA
Janssens, Y., Verbeke, F., Debunne, N., Wynendaele, E., Peremans, K., & De Spiegeleer, B. (2018). Analysis of iodinated quorum sensing peptides by LC-UV/ESI ion trap mass spectrometry. JOURNAL OF PHARMACEUTICAL ANALYSIS, 8(1), 69–74. https://doi.org/10.1016/j.jpha.2017.09.001
Chicago author-date
Janssens, Yorick, Frederick Verbeke, Nathan Debunne, Evelien Wynendaele, Kathelijne Peremans, and Bart De Spiegeleer. 2018. “Analysis of Iodinated Quorum Sensing Peptides by LC-UV/ESI Ion Trap Mass Spectrometry.” JOURNAL OF PHARMACEUTICAL ANALYSIS 8 (1): 69–74. https://doi.org/10.1016/j.jpha.2017.09.001.
Chicago author-date (all authors)
Janssens, Yorick, Frederick Verbeke, Nathan Debunne, Evelien Wynendaele, Kathelijne Peremans, and Bart De Spiegeleer. 2018. “Analysis of Iodinated Quorum Sensing Peptides by LC-UV/ESI Ion Trap Mass Spectrometry.” JOURNAL OF PHARMACEUTICAL ANALYSIS 8 (1): 69–74. doi:10.1016/j.jpha.2017.09.001.
Vancouver
1.
Janssens Y, Verbeke F, Debunne N, Wynendaele E, Peremans K, De Spiegeleer B. Analysis of iodinated quorum sensing peptides by LC-UV/ESI ion trap mass spectrometry. JOURNAL OF PHARMACEUTICAL ANALYSIS. 2018;8(1):69–74.
IEEE
[1]
Y. Janssens, F. Verbeke, N. Debunne, E. Wynendaele, K. Peremans, and B. De Spiegeleer, “Analysis of iodinated quorum sensing peptides by LC-UV/ESI ion trap mass spectrometry,” JOURNAL OF PHARMACEUTICAL ANALYSIS, vol. 8, no. 1, pp. 69–74, 2018.
@article{8531998,
  abstract     = {Five different quorum sensing peptides (QSP) were iodinated using different iodination techniques. These iodinated peptides were analyzed using a C-18 reversed phase HPLC system, applying a linear gradient of water and acetonitrile containing 0.1% (m/v) formic acid as mobile phase. Electrospray ionization (ESI) ion trap mass spectrometry was used for the identification of the modified peptides, while semi-quantification was performed using total ion current (TIC) spectra. Non-iodinated peptides and mono- and di-iodinated peptides (NIP, MIP and DIP respectively) were well separated and eluted in that order. Depending on the used iodination method, iodination yields varied from low (2%) to high (57%).},
  author       = {Janssens, Yorick and Verbeke, Frederick and Debunne, Nathan and Wynendaele, Evelien and Peremans, Kathelijne and De Spiegeleer, Bart},
  issn         = {2095-1779},
  journal      = {JOURNAL OF PHARMACEUTICAL ANALYSIS},
  keywords     = {Peptide iodination,LC-UV/MS,Quorum sensing,Bolton-Hunter,PERFORMANCE LIQUID-CHROMATOGRAPHY,BLOOD-BRAIN-BARRIER,ENTEROCOCCUS-FAECALIS,CHEMICAL SPACE,PROTEINS,PENTAPEPTIDE,BACTERIA,CANCER,I-125},
  language     = {eng},
  number       = {1},
  pages        = {69--74},
  title        = {Analysis of iodinated quorum sensing peptides by LC-UV/ESI ion trap mass spectrometry},
  url          = {http://dx.doi.org/10.1016/j.jpha.2017.09.001},
  volume       = {8},
  year         = {2018},
}

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