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Renal auto-transplantation promotes cortical microvascular network remodeling in a preclinical porcine model

(2017) PLOS ONE. 12(7).
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Abstract
The vascular network is a major target of ischemia-reperfusion, but has been poorly investigated in renal transplantation. The aim of this study was to characterize the remodeling of the renal vascular network that follows ischemia-reperfusion along with the most highly affected cortex section in a preclinical renal transplantation model. There were two experimental groups. The first was a grafted kidney group consisting of large white pigs for which the left kidney was harvested, cold flushed, preserved for 24 h in the University of Wisconsin's preservation solution, and then auto-transplanted (n = 5); the right kidney was removed to mimic the situation of human kidney transplantation. The second group (uni-nephrectomized kidney group) consisted of animals that underwent only right nephrectomy, but not left renal transplantation (n = 5). Three months after autotransplantation, the kidneys were studied by X-ray microcomputed tomography. Vessel morphology and density and tortuosity of the network were analyzed using a 3D image analysis method. Cortical blood flow was determined by laser doppler analysis and renal function and tissue injury assessed by plasma creatinine levels and histological analysis. Renal ischemia-reperfusion led to decreased vascular segment volume associated with fewer vessels of less than 30 mu m, particularly in the inner cortex: 0.79 +/- 0.54% in grafted kidneys vs. 7.06 +/- 1.44% in uni-nephrectomized kidneys, p < 0.05. Vessels showed higher connectivity throughout the cortex (the arborescence factor of the whole cortex was less in grafted than uni-nephrectomized kidneys 0.90 +/- 0.04 vs. 1.07 +/- 0.05, p < 0.05, with an increase in the number of bifurcations). Furthermore, cortical blood flow decreased early in kidney grafts and remained low three months after autotransplantation. The decrease in microvasculature correlated with a deterioration of renal function, proteinuria, and tubular dysfunction, and was associated with the development of fibrous tissue. This work provides new evidence concerning the impact of ischemia-reperfusion injuries on the spectrum of renal vascular diseases and could potentially guide future therapy to preserve microvessels in transplantation ischemia-reperfusion injury.
Keywords
LONG-TERM, COLD ISCHEMIA/REPERFUSION, ALLOGRAFT-REJECTION, ENDOTHELIAL INJURY, PIG KIDNEYS, MICROCIRCULATION, DYSFUNCTION, TRANSPLANTATION, FAILURE, HYPERCHOLESTEROLEMIA

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Chicago
Maïga, Souleymane, Geraldine Allain, Thierry Hauet, Jerome Roumy, Edouard Baulier, Michel Scepi, Manuel Dierick, et al. 2017. “Renal Auto-transplantation Promotes Cortical Microvascular Network Remodeling in a Preclinical Porcine Model.” Plos One 12 (7).
APA
Maïga, S., Allain, G., Hauet, T., Roumy, J., Baulier, E., Scepi, M., Dierick, M., et al. (2017). Renal auto-transplantation promotes cortical microvascular network remodeling in a preclinical porcine model. PLOS ONE, 12(7).
Vancouver
1.
Maïga S, Allain G, Hauet T, Roumy J, Baulier E, Scepi M, et al. Renal auto-transplantation promotes cortical microvascular network remodeling in a preclinical porcine model. PLOS ONE. 2017;12(7).
MLA
Maïga, Souleymane et al. “Renal Auto-transplantation Promotes Cortical Microvascular Network Remodeling in a Preclinical Porcine Model.” PLOS ONE 12.7 (2017): n. pag. Print.
@article{8531552,
  abstract     = {The vascular network is a major target of ischemia-reperfusion, but has been poorly investigated in renal transplantation. The aim of this study was to characterize the remodeling of the renal vascular network that follows ischemia-reperfusion along with the most highly affected cortex section in a preclinical renal transplantation model. There were two experimental groups. The first was a grafted kidney group consisting of large white pigs for which the left kidney was harvested, cold flushed, preserved for 24 h in the University of Wisconsin's preservation solution, and then auto-transplanted (n = 5); the right kidney was removed to mimic the situation of human kidney transplantation. The second group (uni-nephrectomized kidney group) consisted of animals that underwent only right nephrectomy, but not left renal transplantation (n = 5). Three months after autotransplantation, the kidneys were studied by X-ray microcomputed tomography. Vessel morphology and density and tortuosity of the network were analyzed using a 3D image analysis method. Cortical blood flow was determined by laser doppler analysis and renal function and tissue injury assessed by plasma creatinine levels and histological analysis. Renal ischemia-reperfusion led to decreased vascular segment volume associated with fewer vessels of less than 30 mu m, particularly in the inner cortex: 0.79 +/- 0.54% in grafted kidneys vs. 7.06 +/- 1.44% in uni-nephrectomized kidneys, p < 0.05. Vessels showed higher connectivity throughout the cortex (the arborescence factor of the whole cortex was less in grafted than uni-nephrectomized kidneys 0.90 +/- 0.04 vs. 1.07 +/- 0.05, p < 0.05, with an increase in the number of bifurcations). Furthermore, cortical blood flow decreased early in kidney grafts and remained low three months after autotransplantation. The decrease in microvasculature correlated with a deterioration of renal function, proteinuria, and tubular dysfunction, and was associated with the development of fibrous tissue. This work provides new evidence concerning the impact of ischemia-reperfusion injuries on the spectrum of renal vascular diseases and could potentially guide future therapy to preserve microvessels in transplantation ischemia-reperfusion injury.},
  articleno    = {e0181067},
  author       = {Maïga, Souleymane and Allain, Geraldine and Hauet, Thierry and Roumy, Jerome and Baulier, Edouard and Scepi, Michel and Dierick, Manuel and Van Hoorebeke, Luc and Hannaert, Patrick and Guy, Franck and Favreau, Frederic},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keywords     = {LONG-TERM,COLD ISCHEMIA/REPERFUSION,ALLOGRAFT-REJECTION,ENDOTHELIAL INJURY,PIG KIDNEYS,MICROCIRCULATION,DYSFUNCTION,TRANSPLANTATION,FAILURE,HYPERCHOLESTEROLEMIA},
  language     = {eng},
  number       = {7},
  pages        = {16},
  title        = {Renal auto-transplantation promotes cortical microvascular network remodeling in a preclinical porcine model},
  url          = {http://dx.doi.org/10.1371/journal.pone.0181067},
  volume       = {12},
  year         = {2017},
}

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