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Cross-linkable gelatins with superior mechanical properties through carboxylic acid modification : increasing the two-photon polymerization potential

Jasper Van Hoorick UGent, Peter Gruber, Marica Markovic, Maximilian Tromayer, Jürgen Van Erps, Hugo Thienpont UGent, Robert Liska, Aleksandr Ovsianikov, Peter Dubruel UGent and Sandra Van Vlierberghe UGent (2017) BIOMACROMOLECULES. 18(10). p.3260-3272
abstract
The present work reports on the development of photo-cross-linkable gelatins sufficiently versatile to overcome current biopolymer two-photon polymerization (2PP) processing limitations. To this end, both the primary amines as well as the carboxylic acids of gelatin type B were functionalized with photo-cross-linkable moieties (up to 1 mmol/g) resulting in superior and tunable mechanical properties (G' from 5000 to 147000 Pa) enabling efficient 2PP processing. The materials were characterized in depth prior to and after photoinduced cross-linking using fully functionalized gelatin-methacrylamide (gel-MOD) as a benchmark to assess the effect of functionalization on the protein properties, cross-linking efficiency, and mechanical properties. In addition, preliminary experiments on hydrogel films indicated excellent in vitro biocompatibility (close to 100% viability) both in the presence of MC3T3 preosteoblasts and L929 fibroblasts. Moreover, 2PP processing of the novel derivative was superior in terms of applied laser power (>= 40 vs >= 60 mW for gel-MOD at 100 mm/s) as well as post-production swelling (0-20% vs 75-100% for gel-MOD) compared to those of gel-MOD. The reported novel gelatin derivative (gel-MOD-AEMA) proves to be extremely suitable for direct laser writing as both superior mimicry of the applied computer-aided design (CAD) was obtained while maintaining the desired cellular interactivity of the biopolymer. It can be anticipated that the present work will also be applicable to alternative biopolymers mimicking the extra cellular environment such as collagen, elastin, and glycosaminoglycans, thereby expanding current material-related processing limitations in the tissue engineering field.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
TISSUE ENGINEERING APPLICATIONS, RHEOLOGICAL PROPERTIES, ELEGANT TOOL, IN-VITRO, HYDROGELS, SCAFFOLDS, METHACRYLAMIDE, LINKING, CONSTRUCTS, CRYOGELS
journal title
BIOMACROMOLECULES
Biomacromolecules
volume
18
issue
10
pages
3260 - 3272
Web of Science type
Article
Web of Science id
000412864900025
ISSN
1525-7797
1526-4602
DOI
10.1021/acs.biomac.7b00905
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
8531515
handle
http://hdl.handle.net/1854/LU-8531515
date created
2017-09-18 12:33:10
date last changed
2017-11-29 14:56:43
@article{8531515,
  abstract     = {The present work reports on the development of photo-cross-linkable gelatins sufficiently versatile to overcome current biopolymer two-photon polymerization (2PP) processing limitations. To this end, both the primary amines as well as the carboxylic acids of gelatin type B were functionalized with photo-cross-linkable moieties (up to 1 mmol/g) resulting in superior and tunable mechanical properties (G' from 5000 to 147000 Pa) enabling efficient 2PP processing. The materials were characterized in depth prior to and after photoinduced cross-linking using fully functionalized gelatin-methacrylamide (gel-MOD) as a benchmark to assess the effect of functionalization on the protein properties, cross-linking efficiency, and mechanical properties. In addition, preliminary experiments on hydrogel films indicated excellent in vitro biocompatibility (close to 100\% viability) both in the presence of MC3T3 preosteoblasts and L929 fibroblasts. Moreover, 2PP processing of the novel derivative was superior in terms of applied laser power ({\textrangle}= 40 vs {\textrangle}= 60 mW for gel-MOD at 100 mm/s) as well as post-production swelling (0-20\% vs 75-100\% for gel-MOD) compared to those of gel-MOD. The reported novel gelatin derivative (gel-MOD-AEMA) proves to be extremely suitable for direct laser writing as both superior mimicry of the applied computer-aided design (CAD) was obtained while maintaining the desired cellular interactivity of the biopolymer. It can be anticipated that the present work will also be applicable to alternative biopolymers mimicking the extra cellular environment such as collagen, elastin, and glycosaminoglycans, thereby expanding current material-related processing limitations in the tissue engineering field.},
  author       = {Van Hoorick, Jasper and Gruber, Peter and Markovic, Marica and Tromayer, Maximilian and Van Erps, J{\"u}rgen and Thienpont, Hugo and Liska, Robert and Ovsianikov, Aleksandr and Dubruel, Peter and Van Vlierberghe, Sandra},
  issn         = {1525-7797},
  journal      = {BIOMACROMOLECULES},
  keyword      = {TISSUE ENGINEERING APPLICATIONS,RHEOLOGICAL PROPERTIES,ELEGANT TOOL,IN-VITRO,HYDROGELS,SCAFFOLDS,METHACRYLAMIDE,LINKING,CONSTRUCTS,CRYOGELS},
  language     = {eng},
  number       = {10},
  pages        = {3260--3272},
  title        = {Cross-linkable gelatins with superior mechanical properties through carboxylic acid modification : increasing the two-photon polymerization potential},
  url          = {http://dx.doi.org/10.1021/acs.biomac.7b00905},
  volume       = {18},
  year         = {2017},
}

Chicago
Van Hoorick, Jasper, Peter Gruber, Marica Markovic, Maximilian Tromayer, Jürgen Van Erps, Hugo Thienpont, Robert Liska, Aleksandr Ovsianikov, Peter Dubruel, and Sandra Van Vlierberghe. 2017. “Cross-linkable Gelatins with Superior Mechanical Properties Through Carboxylic Acid Modification : Increasing the Two-photon Polymerization Potential.” Biomacromolecules 18 (10): 3260–3272.
APA
Van Hoorick, J., Gruber, P., Markovic, M., Tromayer, M., Van Erps, J., Thienpont, H., Liska, R., et al. (2017). Cross-linkable gelatins with superior mechanical properties through carboxylic acid modification : increasing the two-photon polymerization potential. BIOMACROMOLECULES, 18(10), 3260–3272.
Vancouver
1.
Van Hoorick J, Gruber P, Markovic M, Tromayer M, Van Erps J, Thienpont H, et al. Cross-linkable gelatins with superior mechanical properties through carboxylic acid modification : increasing the two-photon polymerization potential. BIOMACROMOLECULES. 2017;18(10):3260–72.
MLA
Van Hoorick, Jasper, Peter Gruber, Marica Markovic, et al. “Cross-linkable Gelatins with Superior Mechanical Properties Through Carboxylic Acid Modification : Increasing the Two-photon Polymerization Potential.” BIOMACROMOLECULES 18.10 (2017): 3260–3272. Print.