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Real-time analysis of the apical hook development

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Abstract
Mechanisms for cell protection are essential for survival of multicellular organisms. In plants, the apical hook, which is transiently formed in darkness when the germinating seedling penetrates towards the soil surface, plays such protective role and shields the vitally important shoot apical meristem and cotyledons from damage. The apical hook is formed by bending of the upper hypocotyl soon after germination, and it is maintained in a closed stage while the hypocotyl continues to penetrate through the soil and rapidly opens when exposed to light in proximity of the soil surface. To uncover the complex molecular network orchestrating this spatiotemporally tightly coordinated process, monitoring of the apical hook development in real time is indispensable. Here we describe an imaging platform that enables high-resolution kinetic analysis of this dynamic developmental process.
Keywords
Apical hook development, Differential growth, Hormonal cross talk, Phenotype analysis, Real-time imaging

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Chicago
Zhu, Qiang, Petra Zádníková, Dajo Smet, Dominique Van Der Straeten, and Eva Benková. 2017. “Real-time Analysis of the Apical Hook Development.” In Plant Hormones : Methods and Protocols, ed. Jürgen Kleine-Vehn and Michael Sauer, 1497:1–8. New York, NY, USA: Springer.
APA
Zhu, Qiang, Zádníková, P., Smet, D., Van Der Straeten, D., & Benková, E. (2017). Real-time analysis of the apical hook development. In J. Kleine-Vehn & M. Sauer (Eds.), Plant hormones : methods and protocols (Vol. 1497, pp. 1–8). New York, NY, USA: Springer.
Vancouver
1.
Zhu Q, Zádníková P, Smet D, Van Der Straeten D, Benková E. Real-time analysis of the apical hook development. In: Kleine-Vehn J, Sauer M, editors. Plant hormones : methods and protocols. New York, NY, USA: Springer; 2017. p. 1–8.
MLA
Zhu, Qiang, Petra Zádníková, Dajo Smet, et al. “Real-time Analysis of the Apical Hook Development.” Plant Hormones : Methods and Protocols. Ed. Jürgen Kleine-Vehn & Michael Sauer. Vol. 1497. New York, NY, USA: Springer, 2017. 1–8. Print.
@incollection{8531392,
  abstract     = {Mechanisms for cell protection are essential for survival of multicellular organisms. In plants, the apical hook, which is transiently formed in darkness when the germinating seedling penetrates towards the soil surface, plays such protective role and shields the vitally important shoot apical meristem and cotyledons from damage. The apical hook is formed by bending of the upper hypocotyl soon after germination, and it is maintained in a closed stage while the hypocotyl continues to penetrate through the soil and rapidly opens when exposed to light in proximity of the soil surface. To uncover the complex molecular network orchestrating this spatiotemporally tightly coordinated process, monitoring of the apical hook development in real time is indispensable. Here we describe an imaging platform that enables high-resolution kinetic analysis of this dynamic developmental process.},
  author       = {Zhu, Qiang and Z{\'a}dn{\'i}kov{\'a}, Petra and Smet, Dajo and Van Der Straeten, Dominique and Benkov{\'a}, Eva},
  booktitle    = {Plant hormones : methods and protocols},
  editor       = {Kleine-Vehn, J{\"u}rgen and Sauer, Michael},
  isbn         = {9781493964673},
  issn         = {1064-3745},
  keyword      = {Apical hook development,Differential growth,Hormonal cross talk,Phenotype analysis,Real-time imaging},
  language     = {eng},
  pages        = {1--8},
  publisher    = {Springer},
  series       = {Methods in Molecular Biology},
  title        = {Real-time analysis of the apical hook development},
  url          = {http://dx.doi.org/10.1007/978-1-4939-6469-7\_1},
  volume       = {1497},
  year         = {2017},
}

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