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OMSim : a simulator for optical map data

Giles Miclotte UGent, Stéphane Plaisance UGent, Stephane Rombauts UGent, Yves Van de Peer UGent, Pieter Audenaert UGent and Jan Fostier UGent (2017) BIOINFORMATICS. 33(17). p.2740-2742
abstract
Motivation: The Bionano Genomics platform allows for the optical detection of short sequence patterns in very long DNA molecules (up to 2.5 Mbp). Molecules with overlapping patterns can be assembled to generate a consensus optical map of the entire genome. In turn, these optical maps can be used to validate or improve de novo genome assembly projects or to detect large-scale structural variation in genomes. Simulated optical map data can assist in the development and benchmarking of tools that operate on those data, such as alignment and assembly software. Additionally, it can help to optimize the experimental setup for a genome of interest. Such a simulator is currently not available. Results: We have developed a simulator, OMSim, that produces synthetic optical map data that mimics real Bionano Genomics data. These simulated data have been tested for compatibility with the Bionano Genomics Irys software system and the Irys-scaffolding scripts. OMSim is capable of handling very large genomes (over 30 Gbp) with high throughput and low memory requirements.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
IBCN, GENOME
journal title
BIOINFORMATICS
Bioinformatics
volume
33
issue
17
pages
2740 - 2742
Web of Science type
Article
Web of Science id
000408772700018
ISSN
1367-4803
1460-2059
DOI
10.1093/bioinformatics/btx293
project
Bioinformatics: from nucleotids to networks (N2N)
language
English
UGent publication?
yes
classification
A1
copyright statement
Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
id
8531258
handle
http://hdl.handle.net/1854/LU-8531258
date created
2017-09-15 08:23:52
date last changed
2017-10-06 11:38:20
@article{8531258,
  abstract     = {Motivation: The Bionano Genomics platform allows for the optical detection of short sequence patterns in very long DNA molecules (up to 2.5 Mbp). Molecules with overlapping patterns can be assembled to generate a consensus optical map of the entire genome. In turn, these optical maps can be used to validate or improve de novo genome assembly projects or to detect large-scale structural variation in genomes. Simulated optical map data can assist in the development and benchmarking of tools that operate on those data, such as alignment and assembly software. Additionally, it can help to optimize the experimental setup for a genome of interest. Such a simulator is currently not available. 
Results: We have developed a simulator, OMSim, that produces synthetic optical map data that mimics real Bionano Genomics data. These simulated data have been tested for compatibility with the Bionano Genomics Irys software system and the Irys-scaffolding scripts. OMSim is capable of handling very large genomes (over 30 Gbp) with high throughput and low memory requirements.},
  author       = {Miclotte, Giles and Plaisance, St{\'e}phane and Rombauts, Stephane and Van de Peer, Yves and Audenaert, Pieter and Fostier, Jan},
  issn         = {1367-4803},
  journal      = {BIOINFORMATICS},
  keyword      = {IBCN,GENOME},
  language     = {eng},
  number       = {17},
  pages        = {2740--2742},
  title        = {OMSim : a simulator for optical map data},
  url          = {http://dx.doi.org/10.1093/bioinformatics/btx293},
  volume       = {33},
  year         = {2017},
}

Chicago
Miclotte, Giles, Stéphane Plaisance, Stephane Rombauts, Yves Van de Peer, Pieter Audenaert, and Jan Fostier. 2017. “OMSim : a Simulator for Optical Map Data.” Bioinformatics 33 (17): 2740–2742.
APA
Miclotte, G., Plaisance, S., Rombauts, S., Van de Peer, Y., Audenaert, P., & Fostier, J. (2017). OMSim : a simulator for optical map data. BIOINFORMATICS, 33(17), 2740–2742.
Vancouver
1.
Miclotte G, Plaisance S, Rombauts S, Van de Peer Y, Audenaert P, Fostier J. OMSim : a simulator for optical map data. BIOINFORMATICS. 2017;33(17):2740–2.
MLA
Miclotte, Giles, Stéphane Plaisance, Stephane Rombauts, et al. “OMSim : a Simulator for Optical Map Data.” BIOINFORMATICS 33.17 (2017): 2740–2742. Print.