Advanced search
1 file | 672.02 KB

The metabolic response in fish to mildly elevated water temperature relates to species-dependent muscular concentrations of imidazole compounds and free amino acids

Author
Organization
Abstract
Fish species show distinct differences in their muscular concentrations of imidazoles and free amino acids (FAA). This study was conducted to investigate whether metabolic response to mildly elevated water temperature (MEWT) relates to species-dependent muscular concentrations of imidazoles and FAA. Thirteen carp and 17 Nile tilapia, housed one per aquarium, were randomly assigned to either acclimation (25 degrees C) or MEWT (30 degrees C) for 14 days. Main muscular concentrations were histidine (HIS; P < 0.001) in carp versus N-aacetylhistidine (NAH; P < 0.001) and taurine (TAU; P=0.001) in tilapia. Although the sum of imidazole (HIS +NAH) and TAU in muscle remained constant over species and temperatures (12 > 0.05), (NAH+HIS)/TAU ratio was markedly higher in carp versus tilapia, and decreased with MEWT only in carp (P < 0.05). Many of the muscular FAA concentrations were higher in carp than in tilapia (P < 0.05). Plasma acylcarnitine profile suggested a higher use of AA and fatty acids in carp metabolism (P < 0.05). On the contrary, the concentration of 3-hydroxyisovalezylcarnitine, a sink of leucine catabolism, (P=0.009) pointed to avoidance of leucine use in tilapia metabolism. Despite a further increase of plasma longer-chain acylcarnitines in tilapia at MEWT (P=0.009), their corresponding beta-oxidation products (3-hydroxy-longer-chain acylcarnitines) remained constant. Together with higher plasma non-esterified fatty acids (NEFA) in carp (P=0.001), the latter shows that carp, being a fatter fish, more readily mobilises fat than tilapia at MEWT, which coincides with more intensive muscular mobilization of imidazoles. This study demonstrates that fish species differ in their metabolic response to MEWT, which is associated with species-dependent changes in muscle imidazole to taurine ratio.
Keywords
Carp, Histidine, Mildly elevated water temperature, N-a-acetylhistidine, Taurine, Tilapia, N-ALPHA-ACETYLHISTIDINE, TILAPIA OREOCHROMIS-MOSSAMBICUS, HISTIDINE-RELATED COMPOUNDS, MILKFISH CHANOS-CHANOS, COMMON CARP, NILE TILAPIA, JAPANESE FLOUNDER, ATLANTIC SALMON, SKELETAL-MUSCLE, WHITE MUSCLE

Downloads

    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 672.02 KB

Citation

Please use this url to cite or link to this publication:

Chicago
Geda, Fikremariam, Annelies Declercq, Sofie C Remø, Rune Waagbø, Marta Ribeiro Alves Lourenço, and Geert Janssens. 2017. “The Metabolic Response in Fish to Mildly Elevated Water Temperature Relates to Species-dependent Muscular Concentrations of Imidazole Compounds and Free Amino Acids.” Journal of Thermal Biology 65: 57–63.
APA
Geda, F., Declercq, A., Remø, S. C., Waagbø, R., Ribeiro Alves Lourenço, M., & Janssens, G. (2017). The metabolic response in fish to mildly elevated water temperature relates to species-dependent muscular concentrations of imidazole compounds and free amino acids. JOURNAL OF THERMAL BIOLOGY, 65, 57–63.
Vancouver
1.
Geda F, Declercq A, Remø SC, Waagbø R, Ribeiro Alves Lourenço M, Janssens G. The metabolic response in fish to mildly elevated water temperature relates to species-dependent muscular concentrations of imidazole compounds and free amino acids. JOURNAL OF THERMAL BIOLOGY. 2017;65:57–63.
MLA
Geda, Fikremariam, Annelies Declercq, Sofie C Remø, et al. “The Metabolic Response in Fish to Mildly Elevated Water Temperature Relates to Species-dependent Muscular Concentrations of Imidazole Compounds and Free Amino Acids.” JOURNAL OF THERMAL BIOLOGY 65 (2017): 57–63. Print.
@article{8531257,
  abstract     = {Fish species show distinct differences in their muscular concentrations of imidazoles and free amino acids (FAA). This study was conducted to investigate whether metabolic response to mildly elevated water temperature (MEWT) relates to species-dependent muscular concentrations of imidazoles and FAA. Thirteen carp and 17 Nile tilapia, housed one per aquarium, were randomly assigned to either acclimation (25 degrees C) or MEWT (30 degrees C) for 14 days. Main muscular concentrations were histidine (HIS; P {\textlangle} 0.001) in carp versus N-aacetylhistidine (NAH; P {\textlangle} 0.001) and taurine (TAU; P=0.001) in tilapia. Although the sum of imidazole (HIS +NAH) and TAU in muscle remained constant over species and temperatures (12 {\textrangle} 0.05), (NAH+HIS)/TAU ratio was markedly higher in carp versus tilapia, and decreased with MEWT only in carp (P {\textlangle} 0.05). Many of the muscular FAA concentrations were higher in carp than in tilapia (P {\textlangle} 0.05). Plasma acylcarnitine profile suggested a higher use of AA and fatty acids in carp metabolism (P {\textlangle} 0.05). On the contrary, the concentration of 3-hydroxyisovalezylcarnitine, a sink of leucine catabolism, (P=0.009) pointed to avoidance of leucine use in tilapia metabolism. Despite a further increase of plasma longer-chain acylcarnitines in tilapia at MEWT (P=0.009), their corresponding beta-oxidation products (3-hydroxy-longer-chain acylcarnitines) remained constant. Together with higher plasma non-esterified fatty acids (NEFA) in carp (P=0.001), the latter shows that carp, being a fatter fish, more readily mobilises fat than tilapia at MEWT, which coincides with more intensive muscular mobilization of imidazoles. This study demonstrates that fish species differ in their metabolic response to MEWT, which is associated with species-dependent changes in muscle imidazole to taurine ratio.},
  author       = {Geda, Fikremariam and Declercq, Annelies and Rem{\o}, Sofie C and Waagb{\o}, Rune and Ribeiro Alves Louren\c{c}o, Marta and Janssens, Geert},
  issn         = {0306-4565},
  journal      = {JOURNAL OF THERMAL BIOLOGY},
  keyword      = {Carp,Histidine,Mildly elevated water temperature,N-a-acetylhistidine,Taurine,Tilapia,N-ALPHA-ACETYLHISTIDINE,TILAPIA OREOCHROMIS-MOSSAMBICUS,HISTIDINE-RELATED COMPOUNDS,MILKFISH CHANOS-CHANOS,COMMON CARP,NILE TILAPIA,JAPANESE FLOUNDER,ATLANTIC SALMON,SKELETAL-MUSCLE,WHITE MUSCLE},
  language     = {eng},
  pages        = {57--63},
  title        = {The metabolic response in fish to mildly elevated water temperature relates to species-dependent muscular concentrations of imidazole compounds and free amino acids},
  url          = {http://dx.doi.org/10.1016/j.jtherbio.2017.02.004},
  volume       = {65},
  year         = {2017},
}

Altmetric
View in Altmetric
Web of Science
Times cited: