Ghent University Academic Bibliography

Advanced

Multiple PPR protein interactions are involved in the RNA editing system in Arabidopsis mitochondria and plastids

Nuria Andres Colas, Qiang Zhu UGent, Mizuki Takenaka, Bert De Rybel UGent, Dolf Weijers and Dominique Van Der Straeten UGent (2017) PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 114(33). p.8883-8888
abstract
Recent identification of several different types of RNA editing factors in plant organelles suggests complex RNA editosomes within which each factor has a different task. However, the precise protein interactions between the different editing factors are still poorly understood. In this paper, we show that the E+-type pentatricopeptide repeat (PPR) protein SLO2, which lacks a C-terminal cytidine deaminase-like DYW domain, interacts in vivo with the DYW-type PPR protein DYW2 and the P-type PPR protein NUWA in mitochondria, and that the latter enhances the interaction of the former ones. These results may reflect a protein scaffold or complex stabilization role of NUWA between E+-type PPR and DYW2 proteins. Interestingly, DYW2 and NUWA also interact in chloroplasts, and DYW2-GFP overexpressing lines show broad editing defects in both organelles, with predominant specificity for sites edited by E+-type PPR proteins. The latter suggests a coordinated regulation of organellar multiple site editing through DYW2, which probably provides the deaminase activity to E+ editosomes.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
DYW2, NUWA, PPR, RNA editing, SLO2, PENTATRICOPEPTIDE REPEAT PROTEINS, SUBCELLULAR-LOCALIZATION, PLANT-MITOCHONDRIA, DYW DOMAIN, IDENTIFICATION, RECOGNITION, SITE, PEROXISOMES, MACHINERY, THALIANA
journal title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Proc. Natl. Acad. Sci. USA
volume
114
issue
33
pages
8883 - 8888
Web of Science type
Article
Web of Science id
000407610400079
ISSN
0027-8424
DOI
10.1073/pnas.1705815114
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8531126
handle
http://hdl.handle.net/1854/LU-8531126
date created
2017-09-14 09:54:41
date last changed
2017-10-12 07:22:17
@article{8531126,
  abstract     = {Recent identification of several different types of RNA editing factors in plant organelles suggests complex RNA editosomes within which each factor has a different task. However, the precise protein interactions between the different editing factors are still poorly understood. In this paper, we show that the E+-type pentatricopeptide repeat (PPR) protein SLO2, which lacks a C-terminal cytidine deaminase-like DYW domain, interacts in vivo with the DYW-type PPR protein DYW2 and the P-type PPR protein NUWA in mitochondria, and that the latter enhances the interaction of the former ones. These results may reflect a protein scaffold or complex stabilization role of NUWA between E+-type PPR and DYW2 proteins. Interestingly, DYW2 and NUWA also interact in chloroplasts, and DYW2-GFP overexpressing lines show broad editing defects in both organelles, with predominant specificity for sites edited by E+-type PPR proteins. The latter suggests a coordinated regulation of organellar multiple site editing through DYW2, which probably provides the deaminase activity to E+ editosomes.},
  author       = {Andres Colas, Nuria and Zhu, Qiang and Takenaka, Mizuki and De Rybel, Bert and Weijers, Dolf and Van Der Straeten, Dominique},
  issn         = {0027-8424},
  journal      = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},
  keyword      = {DYW2,NUWA,PPR,RNA editing,SLO2,PENTATRICOPEPTIDE REPEAT PROTEINS,SUBCELLULAR-LOCALIZATION,PLANT-MITOCHONDRIA,DYW DOMAIN,IDENTIFICATION,RECOGNITION,SITE,PEROXISOMES,MACHINERY,THALIANA},
  language     = {eng},
  number       = {33},
  pages        = {8883--8888},
  title        = {Multiple PPR protein interactions are involved in the RNA editing system in Arabidopsis mitochondria and plastids},
  url          = {http://dx.doi.org/10.1073/pnas.1705815114},
  volume       = {114},
  year         = {2017},
}

Chicago
Andres Colas, Nuria, Qiang Zhu, Mizuki Takenaka, Bert De Rybel, Dolf Weijers, and Dominique Van Der Straeten. 2017. “Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids.” Proceedings of the National Academy of Sciences of the United States of America 114 (33): 8883–8888.
APA
Andres Colas, N., Zhu, Q., Takenaka, M., De Rybel, B., Weijers, D., & Van Der Straeten, D. (2017). Multiple PPR protein interactions are involved in the RNA editing system in Arabidopsis mitochondria and plastids. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 114(33), 8883–8888.
Vancouver
1.
Andres Colas N, Zhu Q, Takenaka M, De Rybel B, Weijers D, Van Der Straeten D. Multiple PPR protein interactions are involved in the RNA editing system in Arabidopsis mitochondria and plastids. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2017;114(33):8883–8.
MLA
Andres Colas, Nuria, Qiang Zhu, Mizuki Takenaka, et al. “Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 114.33 (2017): 8883–8888. Print.