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Constitutively active Arabidopsis MAP kinase 3 triggers defense responses involving salicylic acid and SUMM2 resistance protein

(2017) PLANT PHYSIOLOGY. 174(2). p.1238-1249
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Abstract
Mitogen-activated protein kinases (MAPKs) are important regulators of plant immunity. Most of the knowledge about the function of these pathways is derived from loss-of-function approaches. Using a gain-of-function approach, we investigated the responses controlled by a constitutively active (CA) MPK3 in Arabidopsis thaliana. CA-MPK3 plants are dwarfed and display a massive derepression of defense genes associated with spontaneous cell death as well as the accumulation of reactive oxygen species, phytoalexins, and the stress-related hormones ethylene and salicylic acid (SA). Remarkably CA-MPK3/sid2 and CA-MPK3/ein2-50 lines, which are impaired in SA synthesis and ethylene signaling, respectively, retain most of the CA-MPK3-associated phenotypes, indicating that the constitutive activity of MPK3 can bypass SA and ethylene signaling to activate defense responses. A comparative analysis of the molecular phenotypes of CA-MPK3 and mpk4 autoimmunity suggested convergence between the MPK3- and MPK4-guarding modules. In support of this model, CA-MPK3 crosses with summ1 and summ2, two known suppressors of mpk4, resulted in a partial reversion of the CA-MPK3 phenotypes. Overall, our data unravel a novel mechanism by which the MAPK signaling network contributes to a robust defense-response system.
Keywords
INDUCED ETHYLENE PRODUCTION, PROGRAMMED CELL-DEATH, INNATE IMMUNITY, PHYTOALEXIN BIOSYNTHESIS, GENE-EXPRESSION, THALIANA, MPK4, CASCADE, ACTIVATION, MEKK1

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Chicago
Genot, Baptiste, Julien Lang, Souha Berriri, Marie Garmier, Françoise Gilard, Stéphanie Pateyron, Katrien Haustraete, Dominique Van Der Straeten, Heribert Hirt, and Jean Colcombet. 2017. “Constitutively Active Arabidopsis MAP Kinase 3 Triggers Defense Responses Involving Salicylic Acid and SUMM2 Resistance Protein.” Plant Physiology 174 (2): 1238–1249.
APA
Genot, B., Lang, J., Berriri, S., Garmier, M., Gilard, F., Pateyron, S., Haustraete, K., et al. (2017). Constitutively active Arabidopsis MAP kinase 3 triggers defense responses involving salicylic acid and SUMM2 resistance protein. PLANT PHYSIOLOGY, 174(2), 1238–1249.
Vancouver
1.
Genot B, Lang J, Berriri S, Garmier M, Gilard F, Pateyron S, et al. Constitutively active Arabidopsis MAP kinase 3 triggers defense responses involving salicylic acid and SUMM2 resistance protein. PLANT PHYSIOLOGY. 2017;174(2):1238–49.
MLA
Genot, Baptiste, Julien Lang, Souha Berriri, et al. “Constitutively Active Arabidopsis MAP Kinase 3 Triggers Defense Responses Involving Salicylic Acid and SUMM2 Resistance Protein.” PLANT PHYSIOLOGY 174.2 (2017): 1238–1249. Print.
@article{8530833,
  abstract     = {Mitogen-activated protein kinases (MAPKs) are important regulators of plant immunity. Most of the knowledge about the function of these pathways is derived from loss-of-function approaches. Using a gain-of-function approach, we investigated the responses controlled by a constitutively active (CA) MPK3 in Arabidopsis thaliana. CA-MPK3 plants are dwarfed and display a massive derepression of defense genes associated with spontaneous cell death as well as the accumulation of reactive oxygen species, phytoalexins, and the stress-related hormones ethylene and salicylic acid (SA). Remarkably CA-MPK3/sid2 and CA-MPK3/ein2-50 lines, which are impaired in SA synthesis and ethylene signaling, respectively, retain most of the CA-MPK3-associated phenotypes, indicating that the constitutive activity of MPK3 can bypass SA and ethylene signaling to activate defense responses. A comparative analysis of the molecular phenotypes of CA-MPK3 and mpk4 autoimmunity suggested convergence between the MPK3- and MPK4-guarding modules. In support of this model, CA-MPK3 crosses with summ1 and summ2, two known suppressors of mpk4, resulted in a partial reversion of the CA-MPK3 phenotypes. Overall, our data unravel a novel mechanism by which the MAPK signaling network contributes to a robust defense-response system.},
  author       = {Genot, Baptiste and Lang, Julien and Berriri, Souha and Garmier, Marie and Gilard, Fran\c{c}oise and Pateyron, St{\'e}phanie and Haustraete, Katrien and Van Der Straeten, Dominique and Hirt, Heribert and Colcombet, Jean},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keyword      = {INDUCED ETHYLENE PRODUCTION,PROGRAMMED CELL-DEATH,INNATE IMMUNITY,PHYTOALEXIN BIOSYNTHESIS,GENE-EXPRESSION,THALIANA,MPK4,CASCADE,ACTIVATION,MEKK1},
  language     = {eng},
  number       = {2},
  pages        = {1238--1249},
  title        = {Constitutively active Arabidopsis MAP kinase 3 triggers defense responses involving salicylic acid and SUMM2 resistance protein},
  url          = {http://dx.doi.org/10.1104/pp.17.00378},
  volume       = {174},
  year         = {2017},
}

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