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Silicon Based System for Single-Nucleotide-Polymorphism Detection: Chip Fabrication and Thermal Characterization of Polymerase Chain Reaction Microchamber

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Abstract
A single nucleotide polymorphism (SNP) is a difference in the DNA sequence of one nucleotide only. We recently proposed a lab-on-a-chip (LoC) system which has the potentiality of fast, sensitive and highly specific SNP detection. Most of the chip components are silicon based and fabricated within a single process. In this paper, the newly developed fabrication method for the silicon chip is presented. The robust and reliable process allows etching structures on the same chip with very different aspect ratios. The characterization of a crucial component to the LoC SNP detector, the microreactor where DNA amplification is performed, is also detailed. Thanks to innovative design and fabrication methodologies, the microreactor has an excellent thermal isolation from the surrounding silicon substrate. This allows for highly localized temperature control. Furthermore, the microreactor is demonstrated to have rapid heating and cooling rates, allowing for rapid amplification of the target DNA fragments. Successful DNA amplification in the microreactor is demonstrated. (C) 2012 The Japan Society of Applied Physics
Keywords
AMPLIFICATION, PCR, CONDUCTIVITY, BIOCHIP, FUTURE

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Chicago
Majeed, Bivragh, Ben Jones, Deniz S. Tezcan, Nina Tutunjyan, Luc Haspeslagh, Sara Peeters, Paolo Fiorini, et al. 2012. “Silicon Based System for Single-Nucleotide-Polymorphism Detection: Chip Fabrication and Thermal Characterization of Polymerase Chain Reaction Microchamber.” Japanese Journal of Applied Physics 51 (4).
APA
Majeed, B., Jones, B., Tezcan, D. S., Tutunjyan, N., Haspeslagh, L., Peeters, S., Fiorini, P., et al. (2012). Silicon Based System for Single-Nucleotide-Polymorphism Detection: Chip Fabrication and Thermal Characterization of Polymerase Chain Reaction Microchamber. JAPANESE JOURNAL OF APPLIED PHYSICS, 51(4).
Vancouver
1.
Majeed B, Jones B, Tezcan DS, Tutunjyan N, Haspeslagh L, Peeters S, et al. Silicon Based System for Single-Nucleotide-Polymorphism Detection: Chip Fabrication and Thermal Characterization of Polymerase Chain Reaction Microchamber. JAPANESE JOURNAL OF APPLIED PHYSICS. Tokyo: Japan Soc Applied Physics; 2012;51(4).
MLA
Majeed, Bivragh, Ben Jones, Deniz S. Tezcan, et al. “Silicon Based System for Single-Nucleotide-Polymorphism Detection: Chip Fabrication and Thermal Characterization of Polymerase Chain Reaction Microchamber.” JAPANESE JOURNAL OF APPLIED PHYSICS 51.4 (2012): n. pag. Print.
@article{8530649,
  abstract     = {A single nucleotide polymorphism (SNP) is a difference in the DNA sequence of one nucleotide only. We recently proposed a lab-on-a-chip (LoC) system which has the potentiality of fast, sensitive and highly specific SNP detection. Most of the chip components are silicon based and fabricated within a single process. In this paper, the newly developed fabrication method for the silicon chip is presented. The robust and reliable process allows etching structures on the same chip with very different aspect ratios. The characterization of a crucial component to the LoC SNP detector, the microreactor where DNA amplification is performed, is also detailed. Thanks to innovative design and fabrication methodologies, the microreactor has an excellent thermal isolation from the surrounding silicon substrate. This allows for highly localized temperature control. Furthermore, the microreactor is demonstrated to have rapid heating and cooling rates, allowing for rapid amplification of the target DNA fragments. Successful DNA amplification in the microreactor is demonstrated. (C) 2012 The Japan Society of Applied Physics},
  articleno    = {04DL01},
  author       = {Majeed, Bivragh and Jones, Ben and Tezcan, Deniz S. and Tutunjyan, Nina and Haspeslagh, Luc and Peeters, Sara and Fiorini, Paolo and Op de Beeck, Maaike and Van Hoof, Chris and Hiraoka, Maki and Tanaka, Hiroyuki and Yamashita, Ichiro},
  issn         = {0021-4922},
  journal      = {JAPANESE JOURNAL OF APPLIED PHYSICS},
  language     = {eng},
  number       = {4},
  pages        = {9},
  publisher    = {Japan Soc Applied Physics},
  title        = {Silicon Based System for Single-Nucleotide-Polymorphism Detection: Chip Fabrication and Thermal Characterization of Polymerase Chain Reaction Microchamber},
  url          = {http://dx.doi.org/10.1143/JJAP.51.04DL01},
  volume       = {51},
  year         = {2012},
}

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