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Measurement of transcripts associated with photorespiration and related redox signaling

Amna M'Hamdi (UGent) , Pavel Kerchev (UGent) , Patrick Willems (UGent) , Graham Noctor and Frank Van Breusegem (UGent)
Author
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Biotechnology for a sustainable economy (Bio-Economy)
Abstract
To study photorespiration and to characterize related components, gene expression analysis is a central approach. An overview of the experimental setup, protocols, and methods we use to investigate photorespiration-associated gene expression is presented. Within this chapter, we describe simple procedures to experimentally alter the photorespiratory flux and provide protocols for transcriptomic analysis with a focus on genes encoding photorespiratory proteins as well as those induced by photorespiratory hydrogen peroxide (H2O2). Examples of typical results are presented and their significance to understanding redox signaling is discussed.
Keywords
CO2, Catalase, H2O2, Photorespiration, Redox signaling, Transcriptomics

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Citation

Please use this url to cite or link to this publication:

Chicago
M’Hamdi, Amna, Pavel Kerchev, Patrick Willems, Graham Noctor, and Frank Van Breusegem. 2017. “Measurement of Transcripts Associated with Photorespiration and Related Redox Signaling.” In Photorespiration : Methods and Protocols, ed. Alisdair R Fernie, Hermann Bauwe, and Andrew PM Weber, 1653:17–29. New York, NY, USA: Springer.
APA
M’Hamdi, Amna, Kerchev, P., Willems, P., Noctor, G., & Van Breusegem, F. (2017). Measurement of transcripts associated with photorespiration and related redox signaling. In Alisdair R Fernie, H. Bauwe, & A. P. Weber (Eds.), Photorespiration : methods and protocols (Vol. 1653, pp. 17–29). New York, NY, USA: Springer.
Vancouver
1.
M’Hamdi A, Kerchev P, Willems P, Noctor G, Van Breusegem F. Measurement of transcripts associated with photorespiration and related redox signaling. In: Fernie AR, Bauwe H, Weber AP, editors. Photorespiration : methods and protocols. New York, NY, USA: Springer; 2017. p. 17–29.
MLA
M’Hamdi, Amna, Pavel Kerchev, Patrick Willems, et al. “Measurement of Transcripts Associated with Photorespiration and Related Redox Signaling.” Photorespiration : Methods and Protocols. Ed. Alisdair R Fernie, Hermann Bauwe, & Andrew PM Weber. Vol. 1653. New York, NY, USA: Springer, 2017. 17–29. Print.
@incollection{8529695,
  abstract     = {To study photorespiration and to characterize related components, gene expression analysis is a central approach. An overview of the experimental setup, protocols, and methods we use to investigate photorespiration-associated gene expression is presented. Within this chapter, we describe simple procedures to experimentally alter the photorespiratory flux and provide protocols for transcriptomic analysis with a focus on genes encoding photorespiratory proteins as well as those induced by photorespiratory hydrogen peroxide (H2O2). Examples of typical results are presented and their significance to understanding redox signaling is discussed.},
  author       = {M'Hamdi, Amna and Kerchev, Pavel and Willems, Patrick and Noctor, Graham and Van Breusegem, Frank},
  booktitle    = {Photorespiration : methods and protocols},
  editor       = {Fernie, Alisdair R and Bauwe, Hermann and Weber, Andrew PM},
  isbn         = {9781493972241},
  issn         = {1064-3745},
  language     = {eng},
  pages        = {17--29},
  publisher    = {Springer},
  series       = {Methods in Molecular Biology},
  title        = {Measurement of transcripts associated with photorespiration and related redox signaling},
  url          = {http://dx.doi.org/10.1007/978-1-4939-7225-8\_2},
  volume       = {1653},
  year         = {2017},
}

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