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Seasonal changes in the biochemical fate of carbon fixed by benthic diatoms in intertidal sediments

(2018) LIMNOLOGY AND OCEANOGRAPHY. 63(2). p.550-569
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Abstract
Benthic diatoms are important primary producers in intertidal marine sediments and form the basis of the food web in these ecosystems. In order to investigate the carbon flow within diatom mats, we performed in situ 13C pulse-chase labeling experiments and followed in detail the biochemical fate of carbon fixed by the diatoms for five consecutive days. These labeling experiments were done at approximately 2-monthly intervals during 1 yr in order to cover seasonal variations. The fixed carbon was recovered in individual carbohydrates including extracellular polymeric substances (EPS), amino acids, fatty acids, and nucleic acid bases. In addition, we assessed a variety of environmental parameters and photosynthetic characteristics. The fixed carbon was initially mainly stored as carbohydrate (glucose) while nitrogen-rich compounds (e.g., amino acids and RNA/DNA) were produced more slowly. During the year, the diatoms distributed the photosynthetically fixed carbon differently among the various carbon pools that were measured. In summer, the diatoms decreased carbon fixation and accumulated relatively more lipid as a storage compound (27% 6 2% vs. 12% 6 5% in other seasons). The percentage of fixed carbon that was excreted as EPS was lower in summer compared to other seasons, amounting 9% 6 4% and 21% 6 6%, respectively. Hence, it seemed that the physiology of the microphytobenthos was different during summer and caused by higher light intensity and a shift in nitrogen source.
Keywords
EXTRACELLULAR ORGANIC-CARBON, POLYMERIC SUBSTANCES EPS, RATIO MASS-SPECTROMETRY, CO-INERTIA ANALYSIS, PHAEODACTYLUM-TRICORNUTUM, THALASSIOSIRA-PSEUDONANA, VARIABLE FLUORESCENCE, AQUATIC ECOSYSTEMS, MIGRATORY RHYTHMS, SURFACE SEDIMENTS

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Citation

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Chicago
Moerdijk-Poortvliet, Tanja CW, Peter van Breugel, Koen Sabbe, Olivier Beauchard, Lucas J Stal, and Henricus TS Boschker. 2018. “Seasonal Changes in the Biochemical Fate of Carbon Fixed by Benthic Diatoms in Intertidal Sediments.” Limnology and Oceanography 63 (2): 550–569.
APA
Moerdijk-Poortvliet, T. C., van Breugel, P., Sabbe, K., Beauchard, O., Stal, L. J., & Boschker, H. T. (2018). Seasonal changes in the biochemical fate of carbon fixed by benthic diatoms in intertidal sediments. LIMNOLOGY AND OCEANOGRAPHY, 63(2), 550–569.
Vancouver
1.
Moerdijk-Poortvliet TC, van Breugel P, Sabbe K, Beauchard O, Stal LJ, Boschker HT. Seasonal changes in the biochemical fate of carbon fixed by benthic diatoms in intertidal sediments. LIMNOLOGY AND OCEANOGRAPHY. 2018;63(2):550–69.
MLA
Moerdijk-Poortvliet, Tanja CW, Peter van Breugel, Koen Sabbe, et al. “Seasonal Changes in the Biochemical Fate of Carbon Fixed by Benthic Diatoms in Intertidal Sediments.” LIMNOLOGY AND OCEANOGRAPHY 63.2 (2018): 550–569. Print.
@article{8529599,
  abstract     = {Benthic diatoms are important primary producers in intertidal marine sediments and form the basis of the food web in these ecosystems. In order to investigate the carbon flow within diatom mats, we performed in situ 13C pulse-chase labeling experiments and followed in detail the biochemical fate of carbon fixed by the diatoms for five consecutive days. These labeling experiments were done at approximately 2-monthly intervals during 1 yr in order to cover seasonal variations. The fixed carbon was recovered in individual carbohydrates including extracellular polymeric substances (EPS), amino acids, fatty acids, and nucleic acid bases. In addition, we assessed a variety of environmental parameters and photosynthetic characteristics. The fixed carbon was initially mainly stored as carbohydrate (glucose) while nitrogen-rich compounds (e.g., amino acids and RNA/DNA) were produced more slowly. During the year, the diatoms distributed the photosynthetically fixed carbon differently among the various carbon pools that were measured. In summer, the diatoms decreased carbon fixation and accumulated relatively more lipid as a storage compound (27\% 6 2\% vs. 12\% 6 5\% in other seasons). The percentage of fixed carbon that was excreted as EPS was lower in summer compared to other seasons, amounting 9\% 6 4\% and 21\% 6 6\%, respectively. Hence, it seemed that the physiology of the microphytobenthos was different during summer and caused by higher light intensity and a shift in nitrogen source.},
  author       = {Moerdijk-Poortvliet, Tanja CW and van Breugel, Peter and Sabbe, Koen and Beauchard, Olivier and Stal, Lucas J and Boschker, Henricus TS},
  issn         = {0024-3590},
  journal      = {LIMNOLOGY AND OCEANOGRAPHY},
  keyword      = {EXTRACELLULAR ORGANIC-CARBON,POLYMERIC SUBSTANCES EPS,RATIO MASS-SPECTROMETRY,CO-INERTIA ANALYSIS,PHAEODACTYLUM-TRICORNUTUM,THALASSIOSIRA-PSEUDONANA,VARIABLE FLUORESCENCE,AQUATIC ECOSYSTEMS,MIGRATORY RHYTHMS,SURFACE SEDIMENTS},
  language     = {eng},
  number       = {2},
  pages        = {550--569},
  title        = {Seasonal changes in the biochemical fate of carbon fixed by benthic diatoms in intertidal sediments},
  url          = {http://dx.doi.org/10.1002/lno.10648},
  volume       = {63},
  year         = {2018},
}

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