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Sensitive albuminuria analysis using dye-binding based test strips

Joris Delanghe (UGent) , Jonas Himpe (UGent) , Naomi De Cock, Sigurd Delanghe (UGent) , Kevin De Herde (UGent) , Veronique Stove (UGent) and Marijn Speeckaert (UGent)
(2017) CLINICA CHIMICA ACTA. 471. p.107-112
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Abstract
Background: Populations at increased risk for chronic kidney disease should be screened for albuminuria. Possibilities of advanced urine strip readers based on complementary metal oxide semiconductor (CMOS) sensor technology were investigated for obtaining quantitative albuminuria results. Methods: Reflectance data of test strips (Sysmex UFC 3500 reader + CMOS) were compared with albuminuria (BNII) and with proteinuria (Cobas 8000). Urinary creatinine was assayed using a Jaffe-based creatinine assay (Cobas 8000). Results: Calibration curve was made between 11.5 and 121.5 mg/L with detection limit of 5.5 mg/L. Within-run CV values of reflectance data were 0.21% (UC-Control L; 10 mg/L) and 0.37% (UC-Control H; > 150 mg/L) for albumin, and 0.71%/3.97% for creatinine. Between-run CV values were 0.24%/0.42% for albumin and 0.93%/5.13% for creatinine. A strong correlation (r = 0.92) was obtained between albuminuria (BNII) and protein strip reflectance data. Creatinine reflectance data correlated well with Jaffe-based urinary creatinine data (r = 0.90). Albumin:creatinine ratio obtained by test strip and by wet chemistry showed a good correlation (r = 0.59). Carbamylated, glycated and partially hydrolyzed isoforms of albumin could be detected by test strip. Conclusions: Dye-binding based albumin test strip assay in combination with a CMOS based reader would potentially allow quantitative analysis of albuminuria and determination of albumin:creatinine ratio.
Keywords
Albuminuria, Chronic kidney disease, Diabetes mellitus, Reflectance data, Test strips, CHRONIC KIDNEY-DISEASE, MULTICENTER EVALUATION, POSITION STATEMENT, URINE ALBUMIN, CARE, MICROALBUMINURIA, PROTEINURIA, URINALYSIS, POINT

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Chicago
Delanghe, Joris, Jonas Himpe, Naomi De Cock, Sigurd Delanghe, Kevin De Herde, Veronique Stove, and Marijn Speeckaert. 2017. “Sensitive Albuminuria Analysis Using Dye-binding Based Test Strips.” Clinica Chimica Acta 471: 107–112.
APA
Delanghe, J., Himpe, J., De Cock, N., Delanghe, S., De Herde, K., Stove, V., & Speeckaert, M. (2017). Sensitive albuminuria analysis using dye-binding based test strips. CLINICA CHIMICA ACTA, 471, 107–112.
Vancouver
1.
Delanghe J, Himpe J, De Cock N, Delanghe S, De Herde K, Stove V, et al. Sensitive albuminuria analysis using dye-binding based test strips. CLINICA CHIMICA ACTA. 2017;471:107–12.
MLA
Delanghe, Joris et al. “Sensitive Albuminuria Analysis Using Dye-binding Based Test Strips.” CLINICA CHIMICA ACTA 471 (2017): 107–112. Print.
@article{8529424,
  abstract     = {Background: Populations at increased risk for chronic kidney disease should be screened for albuminuria. Possibilities of advanced urine strip readers based on complementary metal oxide semiconductor (CMOS) sensor technology were investigated for obtaining quantitative albuminuria results. 
Methods: Reflectance data of test strips (Sysmex UFC 3500 reader + CMOS) were compared with albuminuria (BNII) and with proteinuria (Cobas 8000). Urinary creatinine was assayed using a Jaffe-based creatinine assay (Cobas 8000). 
Results: Calibration curve was made between 11.5 and 121.5 mg/L with detection limit of 5.5 mg/L. Within-run CV values of reflectance data were 0.21\% (UC-Control L; 10 mg/L) and 0.37\% (UC-Control H; {\textrangle} 150 mg/L) for albumin, and 0.71\%/3.97\% for creatinine. Between-run CV values were 0.24\%/0.42\% for albumin and 0.93\%/5.13\% for creatinine. A strong correlation (r = 0.92) was obtained between albuminuria (BNII) and protein strip reflectance data. Creatinine reflectance data correlated well with Jaffe-based urinary creatinine data (r = 0.90). Albumin:creatinine ratio obtained by test strip and by wet chemistry showed a good correlation (r = 0.59). Carbamylated, glycated and partially hydrolyzed isoforms of albumin could be detected by test strip. 
Conclusions: Dye-binding based albumin test strip assay in combination with a CMOS based reader would potentially allow quantitative analysis of albuminuria and determination of albumin:creatinine ratio.},
  author       = {Delanghe, Joris and Himpe, Jonas and De Cock, Naomi and Delanghe, Sigurd and De Herde, Kevin and Stove, Veronique and Speeckaert, Marijn},
  issn         = {0009-8981},
  journal      = {CLINICA CHIMICA ACTA},
  language     = {eng},
  pages        = {107--112},
  title        = {Sensitive albuminuria analysis using dye-binding based test strips},
  url          = {http://dx.doi.org/10.1016/j.cca.2017.05.032.},
  volume       = {471},
  year         = {2017},
}

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