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Studying the effect of administration route and treatment dose on the selection of enrofloxacin resistance in commensal Escherichia coli in broilers

Ilias Chantziaras (UGent) , Annemieke Smet (UGent) , Freddy Haesebrouck (UGent) , Filip Boyen (UGent) and Jeroen Dewulf (UGent)
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Abstract
Objectives: Factors potentially contributing to fluoroquinolone resistance selection in commensal Escherichia coli strains in poultry were studied through a series of in vivo experiments. The effect of the initial prevalence of enrofloxacin resistance in the E. coli gut microbiota, effect of the bacterial fitness of the enrofloxacin-resistant strain and effect of treatment with enrofloxacin (effect of dose and effect of route of administration) were assessed. Methods: Four in vivo studies with broiler chickens were performed. Right after hatching, the chicks were inoculated with either a bacteriologically fit or a bacteriologically non-fit fluoroquinolone-resistant strain as either a minority or the majority of the total E. coli population. Six days later, the chicks were treated for three consecutive days either orally or parenterally and using three different doses (under-, correct- and over-dose) of enrofloxacin. The faecal shedding of E. coli strains was quantified by plating on agar plates either supplemented or not supplemented with enrofloxacin. Linear mixed models were used to assess the effect of the aforementioned variables on the selection of enrofloxacin resistance. Results: The factors that significantly contributed were treatment (P < 0.001), bacterial fitness of the resistant donor strain (P < 0.001), administration route (P = 0.052) and interactions between bacterial fitness and administration route (P < 0.001). Conclusions: In the currently used models, fluoroquinolone resistance selection was influenced by treatment, bacterial fitness of the inoculation strain and administration route. The use of oral treatment seems to select more for fluoroquinolone resistance, particularly in the model where a non-fit strain was used for inoculation.
Keywords
MEDIATED QUINOLONE RESISTANCE, MUTANT PREVENTION CONCENTRATIONS, ANTIBIOTIC-RESISTANCE, FLUOROQUINOLONE RESISTANCE, ANTIMICROBIAL RESISTANCE, FITNESS COST, COMPENSATORY MUTATIONS, POPULATION-GENETICS, DRUG EXPOSURE, UNITED-STATES

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Please use this url to cite or link to this publication:

Chicago
Chantziaras, Ilias, Annemieke Smet, Freddy Haesebrouck, Filip Boyen, and Jeroen Dewulf. 2017. “Studying the Effect of Administration Route and Treatment Dose on the Selection of Enrofloxacin Resistance in Commensal Escherichia Coli in Broilers.” Journal of Antimicrobial Chemotherapy 72 (7): 1991–2001.
APA
Chantziaras, I., Smet, A., Haesebrouck, F., Boyen, F., & Dewulf, J. (2017). Studying the effect of administration route and treatment dose on the selection of enrofloxacin resistance in commensal Escherichia coli in broilers. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 72(7), 1991–2001.
Vancouver
1.
Chantziaras I, Smet A, Haesebrouck F, Boyen F, Dewulf J. Studying the effect of administration route and treatment dose on the selection of enrofloxacin resistance in commensal Escherichia coli in broilers. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY. 2017;72(7):1991–2001.
MLA
Chantziaras, Ilias, Annemieke Smet, Freddy Haesebrouck, et al. “Studying the Effect of Administration Route and Treatment Dose on the Selection of Enrofloxacin Resistance in Commensal Escherichia Coli in Broilers.” JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY 72.7 (2017): 1991–2001. Print.
@article{8529000,
  abstract     = {Objectives: Factors potentially contributing to fluoroquinolone resistance selection in commensal Escherichia coli strains in poultry were studied through a series of in vivo experiments. The effect of the initial prevalence of enrofloxacin resistance in the E. coli gut microbiota, effect of the bacterial fitness of the enrofloxacin-resistant strain and effect of treatment with enrofloxacin (effect of dose and effect of route of administration) were assessed. 
Methods: Four in vivo studies with broiler chickens were performed. Right after hatching, the chicks were inoculated with either a bacteriologically fit or a bacteriologically non-fit fluoroquinolone-resistant strain as either a minority or the majority of the total E. coli population. Six days later, the chicks were treated for three consecutive days either orally or parenterally and using three different doses (under-, correct- and over-dose) of enrofloxacin. The faecal shedding of E. coli strains was quantified by plating on agar plates either supplemented or not supplemented with enrofloxacin. Linear mixed models were used to assess the effect of the aforementioned variables on the selection of enrofloxacin resistance. 
Results: The factors that significantly contributed were treatment (P {\textlangle} 0.001), bacterial fitness of the resistant donor strain (P {\textlangle} 0.001), administration route (P = 0.052) and interactions between bacterial fitness and administration route (P {\textlangle} 0.001). 
Conclusions: In the currently used models, fluoroquinolone resistance selection was influenced by treatment, bacterial fitness of the inoculation strain and administration route. The use of oral treatment seems to select more for fluoroquinolone resistance, particularly in the model where a non-fit strain was used for inoculation.},
  author       = {Chantziaras, Ilias and Smet, Annemieke and Haesebrouck, Freddy and Boyen, Filip and Dewulf, Jeroen},
  issn         = {0305-7453},
  journal      = {JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY},
  language     = {eng},
  number       = {7},
  pages        = {1991--2001},
  title        = {Studying the effect of administration route and treatment dose on the selection of enrofloxacin resistance in commensal Escherichia coli in broilers},
  url          = {http://dx.doi.org/10.1093/jac/dkx104},
  volume       = {72},
  year         = {2017},
}

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