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Antibodies raised against the flagellar pocket fraction of Trypanosoma brucei preferentially recognize HSP60 in cDNA expression library

(2003) PARASITE IMMUNOLOGY. 22(12). p.639-650
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Abstract
A purified flagellar pocket fraction of the Trypanosoma brucei AnTat 1.1E clone was used for the generation of polyclonal antiserum in rats. Anti-flagellar pocket antibodies present in this serum recognized several proteins distinct from the major variant surface glycoprotein (VSG). In Balb/c mice, flagellar pocket immunization resulted in partial resistance towards the challenge with a low dose of parasites. This was accompanied by the induction of specific IgG2a antibodies. In art attempt to discover protective parasite antigens, antiflagellar pocket serum was used for the screening of a T. brucei bloodstream form cDNA library constructed in the lambda gt11 bacteriophage expression system. Through antibody panning and VSG elimination, 15 specific cDNA inserts were selected. Most intriguing was the observation that in addition to two clones encoding the invariant surface glycoprotein 75 (ISG75), 10 out of 15 independently selected cDNA inserts encoded the trypanosome heat shock protein 60 (tHSP60).
Keywords
Trypanosoma brucei, lambda gt11 cDNA library, screening, heat shock protein 60 (HSP60), VARIANT SURFACE GLYCOPROTEINS, BINDING PROTEIN COMPLEX, AFRICAN TRYPANOSOMES, TRANSFERRIN RECEPTOR, FORM, MEMBRANE, IDENTIFICATION, LOCALIZATION, ENDOCYTOSIS, GENE

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Chicago
Radwanska, Magdalena, Stefan Magez, Nathalie Dumont, Annette Pays, Derek Nolan, and Etienne Pays. 2003. “Antibodies Raised Against the Flagellar Pocket Fraction of Trypanosoma Brucei Preferentially Recognize HSP60 in cDNA Expression Library.” Parasite Immunology 22 (12): 639–650.
APA
Radwanska, M., Magez, S., Dumont, N., Pays, A., Nolan, D., & Pays, E. (2003). Antibodies raised against the flagellar pocket fraction of Trypanosoma brucei preferentially recognize HSP60 in cDNA expression library. PARASITE IMMUNOLOGY, 22(12), 639–650.
Vancouver
1.
Radwanska M, Magez S, Dumont N, Pays A, Nolan D, Pays E. Antibodies raised against the flagellar pocket fraction of Trypanosoma brucei preferentially recognize HSP60 in cDNA expression library. PARASITE IMMUNOLOGY. 2003;22(12):639–50.
MLA
Radwanska, Magdalena et al. “Antibodies Raised Against the Flagellar Pocket Fraction of Trypanosoma Brucei Preferentially Recognize HSP60 in cDNA Expression Library.” PARASITE IMMUNOLOGY 22.12 (2003): 639–650. Print.
@article{8528997,
  abstract     = {A purified flagellar pocket fraction of the Trypanosoma brucei AnTat 1.1E clone was used for the generation of polyclonal antiserum in rats. Anti-flagellar pocket antibodies present in this serum recognized several proteins distinct from the major variant surface glycoprotein (VSG). In Balb/c mice, flagellar pocket immunization resulted in partial resistance towards the challenge with a low dose of parasites. This was accompanied by the induction of specific IgG2a antibodies. In art attempt to discover protective parasite antigens, antiflagellar pocket serum was used for the screening of a T. brucei bloodstream form cDNA library constructed in the lambda gt11 bacteriophage expression system. Through antibody panning and VSG elimination, 15 specific cDNA inserts were selected. Most intriguing was the observation that in addition to two clones encoding the invariant surface glycoprotein 75 (ISG75), 10 out of 15 independently selected cDNA inserts encoded the trypanosome heat shock protein 60 (tHSP60).},
  author       = {Radwanska, Magdalena and Magez, Stefan and Dumont, Nathalie and Pays, Annette and Nolan, Derek and Pays, Etienne},
  issn         = {0141-9838},
  journal      = {PARASITE IMMUNOLOGY},
  language     = {eng},
  number       = {12},
  pages        = {639--650},
  title        = {Antibodies raised against the flagellar pocket fraction of Trypanosoma brucei preferentially recognize HSP60 in cDNA expression library},
  url          = {http://dx.doi.org/10.1046/j.1365-3024.2000.00348.x},
  volume       = {22},
  year         = {2003},
}

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