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Utility of integrated HIV-1 DNA quantification in cure studies

(2017) FUTURE VIROLOGY. 12(4). p.15-225
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Abstract
Numerous HIV-1 curative strategies have been proposed to eradicate the virus reservoir pool that remains integrated within target cells, despite successful antiretroviral therapy. To test the impact of such interventions on this reservoir, a universal marker of persistence is needed. Quantifying integrated HIV-1 DNA load has been proposed as a strong virological marker. In this paper, we provide a detailed description of the most commonly used assays to quantify integrated HIV-1 DNA and applications in relevant clinical studies produced over the last 20 years with a major focus on the recent literature. We discuss the potential for using this marker of virological persistence and the technical limitations that need to be addressed.
Keywords
Alu-gag PCR, HIV-1 reservoir, integrated HIV-1 DNA, replication competency, CD4(+) T-CELLS, SUPPRESSIVE ANTIRETROVIRAL THERAPY, HISTONE DEACETYLASE INHIBITORS, LATENT RESERVOIR, VIRAL PERSISTENCE, PERIPHERAL-BLOOD, RALTEGRAVIR INTENSIFICATION, ONGOING REPLICATION, DISEASE PROGRESSION, TYPE-1 REPLICATION

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Citation

Please use this url to cite or link to this publication:

MLA
Ruggiero, Alessandra et al. “Utility of Integrated HIV-1 DNA Quantification in Cure Studies.” FUTURE VIROLOGY 12.4 (2017): 15–225. Print.
APA
Ruggiero, A., Malatinková, E., Rutsaert, S., Paxton, W. A., Vandekerckhove, L., & De Spiegelaere, W. (2017). Utility of integrated HIV-1 DNA quantification in cure studies. FUTURE VIROLOGY, 12(4), 15–225.
Chicago author-date
Ruggiero, Alessandra, Eva Malatinková, Sofie Rutsaert, William A Paxton, Linos Vandekerckhove, and Ward De Spiegelaere. 2017. “Utility of Integrated HIV-1 DNA Quantification in Cure Studies.” Future Virology 12 (4): 15–225.
Chicago author-date (all authors)
Ruggiero, Alessandra, Eva Malatinková, Sofie Rutsaert, William A Paxton, Linos Vandekerckhove, and Ward De Spiegelaere. 2017. “Utility of Integrated HIV-1 DNA Quantification in Cure Studies.” Future Virology 12 (4): 15–225.
Vancouver
1.
Ruggiero A, Malatinková E, Rutsaert S, Paxton WA, Vandekerckhove L, De Spiegelaere W. Utility of integrated HIV-1 DNA quantification in cure studies. FUTURE VIROLOGY. 2017;12(4):15–225.
IEEE
[1]
A. Ruggiero, E. Malatinková, S. Rutsaert, W. A. Paxton, L. Vandekerckhove, and W. De Spiegelaere, “Utility of integrated HIV-1 DNA quantification in cure studies,” FUTURE VIROLOGY, vol. 12, no. 4, pp. 15–225, 2017.
@article{8528947,
  abstract     = {Numerous HIV-1 curative strategies have been proposed to eradicate the virus reservoir pool that remains integrated within target cells, despite successful antiretroviral therapy. To test the impact of such interventions on this reservoir, a universal marker of persistence is needed. Quantifying integrated HIV-1 DNA load has been proposed as a strong virological marker. In this paper, we provide a detailed description of the most commonly used assays to quantify integrated HIV-1 DNA and applications in relevant clinical studies produced over the last 20 years with a major focus on the recent literature. We discuss the potential for using this marker of virological persistence and the technical limitations that need to be addressed.},
  author       = {Ruggiero, Alessandra and Malatinková, Eva and Rutsaert, Sofie and Paxton, William A and Vandekerckhove, Linos and De Spiegelaere, Ward},
  issn         = {1746-0794},
  journal      = {FUTURE VIROLOGY},
  keywords     = {Alu-gag PCR,HIV-1 reservoir,integrated HIV-1 DNA,replication competency,CD4(+) T-CELLS,SUPPRESSIVE ANTIRETROVIRAL THERAPY,HISTONE DEACETYLASE INHIBITORS,LATENT RESERVOIR,VIRAL PERSISTENCE,PERIPHERAL-BLOOD,RALTEGRAVIR INTENSIFICATION,ONGOING REPLICATION,DISEASE PROGRESSION,TYPE-1 REPLICATION},
  language     = {eng},
  number       = {4},
  pages        = {15--225},
  title        = {Utility of integrated HIV-1 DNA quantification in cure studies},
  url          = {http://dx.doi.org/10.2217/fvl-2016-0130},
  volume       = {12},
  year         = {2017},
}

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