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A new transcript in the TCRB locus unveils the human ortholog of the mouse pre-Dß1 promoter

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Abstract
Introduction: While most transcripts arising from the human T Cell Receptor locus reflect fully rearranged genes, several germline transcripts have been identified. We describe a new germline transcript arising from the human TCRB locus. Methods: cDNA sequencing, promoter, and gene expression analyses were used to characterize the new transcript. Results: The new germline transcript encoded by the human TCRB locus consists of a new exon of 103bp, which we named TRBX1 (X1), spliced with the first exon of gene segments C ss 1 or C ss 2. X1 is located upstream of gene segment D ss 1 and is therefore deleted from a V-DJ rearranged TCRB locus. The X1-C ss transcripts do not appear to code for a protein. We define their transcription start and minimal promoter. These transcripts are found in populations of mature T lymphocytes from blood or tissues and in T cell clones with a monoallelic TCRB rearrangement. In immature thymocytes, they are already detectable in CD1a(-)CD34(+)CD4(-)CD8(-) cells, therefore before completion of the TCRB rearrangements. Conclusions: The X1 promoter appears to be the ortholog of the mouse pre-D ss 1 promoter (PD ss 1). Like PD ss 1, its activation is regulated by E ss in T cells and might facilitate the TCRB rearrangement process by contributing to the accessibility of the D ss 1 locus.
Keywords
Allelic inclusion, human TCRB locus, locus accessibility, PD ss 1 germline transcripts, T cells, thymocytes, TCRB transcripts, T-CELL-RECEPTOR, ACUTE LYMPHOBLASTIC LEUKEMIAS, BETA-GENE SEGMENTS, GERMLINE TRANSCRIPTION, V(D)J RECOMBINATION, CHAIN GENE, EXPRESSION, IMMUNOGLOBULIN, REARRANGEMENT, ACCESSIBILITY

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Chicago
Lethé, Bernard, SYLVIA SNAUWAERT, Orian Bricard, David Schröder, Tiphanie Gomard, Gérald Hames, Catherine Muller, et al. 2017. “A New Transcript in the TCRB Locus Unveils the Human Ortholog of the Mouse pre-Dß1 Promoter.” Immunity Inflammation and Disease 5 (3): 346–354.
APA
Lethé, B., SNAUWAERT, S., Bricard, O., Schröder, D., Gomard, T., Hames, G., Muller, C., et al. (2017). A new transcript in the TCRB locus unveils the human ortholog of the mouse pre-Dß1 promoter. IMMUNITY INFLAMMATION AND DISEASE, 5(3), 346–354.
Vancouver
1.
Lethé B, SNAUWAERT S, Bricard O, Schröder D, Gomard T, Hames G, et al. A new transcript in the TCRB locus unveils the human ortholog of the mouse pre-Dß1 promoter. IMMUNITY INFLAMMATION AND DISEASE. 2017;5(3):346–54.
MLA
Lethé, Bernard, SYLVIA SNAUWAERT, Orian Bricard, et al. “A New Transcript in the TCRB Locus Unveils the Human Ortholog of the Mouse pre-Dß1 Promoter.” IMMUNITY INFLAMMATION AND DISEASE 5.3 (2017): 346–354. Print.
@article{8528433,
  abstract     = {Introduction: While most transcripts arising from the human T Cell Receptor locus reflect fully rearranged genes, several germline transcripts have been identified. We describe a new germline transcript arising from the human TCRB locus. 
Methods: cDNA sequencing, promoter, and gene expression analyses were used to characterize the new transcript. 
Results: The new germline transcript encoded by the human TCRB locus consists of a new exon of 103bp, which we named TRBX1 (X1), spliced with the first exon of gene segments C ss 1 or C ss 2. X1 is located upstream of gene segment D ss 1 and is therefore deleted from a V-DJ rearranged TCRB locus. The X1-C ss transcripts do not appear to code for a protein. We define their transcription start and minimal promoter. These transcripts are found in populations of mature T lymphocytes from blood or tissues and in T cell clones with a monoallelic TCRB rearrangement. In immature thymocytes, they are already detectable in CD1a(-)CD34(+)CD4(-)CD8(-) cells, therefore before completion of the TCRB rearrangements. 
Conclusions: The X1 promoter appears to be the ortholog of the mouse pre-D ss 1 promoter (PD ss 1). Like PD ss 1, its activation is regulated by E ss in T cells and might facilitate the TCRB rearrangement process by contributing to the accessibility of the D ss 1 locus.},
  author       = {Leth{\'e}, Bernard and SNAUWAERT, SYLVIA and Bricard, Orian and Schr{\"o}der, David and Gomard, Tiphanie and Hames, G{\'e}rald and Muller, Catherine and Lurquin, Christophe and Gauthy, Emilie and Essaghir, Ahmed and Vandekerckhove, Bart and Coulie, Pierre G},
  issn         = {2050-4527},
  journal      = {IMMUNITY INFLAMMATION AND DISEASE},
  keyword      = {Allelic inclusion,human TCRB locus,locus accessibility,PD ss 1 germline transcripts,T cells,thymocytes,TCRB transcripts,T-CELL-RECEPTOR,ACUTE LYMPHOBLASTIC LEUKEMIAS,BETA-GENE SEGMENTS,GERMLINE TRANSCRIPTION,V(D)J RECOMBINATION,CHAIN GENE,EXPRESSION,IMMUNOGLOBULIN,REARRANGEMENT,ACCESSIBILITY},
  language     = {eng},
  number       = {3},
  pages        = {346--354},
  title        = {A new transcript in the TCRB locus unveils the human ortholog of the mouse pre-D{\ss}1 promoter},
  url          = {http://dx.doi.org/10.1002/iid3.172},
  volume       = {5},
  year         = {2017},
}

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