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Functional characterization of BdB1, a well-conserved carboxylesterase among tephritid fruit flies associated with malathion resistance in Bactrocera dorsalis (Hendel)

Luoluo Wang UGent, Xue-Ping Lu, Guy Smagghe UGent, Li-Wei Meng and Jin-Jun Wang (2017) COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY. 200. p.1-8
abstract
There are many evidences that insect carboxylesterase possess important physiological roles in xenobiotic metabolism and are implicated in the detoxification of organophosphate (OP) insecticides. Despite the ongoing resistance development in the oriental fruit fly, Bactrocera dorsalis (Hendel), the molecular basis of carboxylesterase and its ability to confer OP resistance remain largely obscure. This study was initiated to provide a better understanding of carboxylesterase-mediated resistance mechanism in a tephritid pest fly. Here, we narrow this research gap by demonstrating a well-conserved esterase B1 gene, BdB1, mediates malathion resistance development via gene upregulation with the use of a laboratory selected malathion-resistant strain (MR) of B. dorsalis. No sequence mutation of BdB1 was detected between MR and the susceptible strain (MS) of B. dorsalis. BdB1 is predominantly expressed in the midgut, a key insect tissue for detoxification. As compared with transcripts in MS, BdB1 was significantly more abundant in multiple tissues in the MR. RNA interference (RNAi)-mediated knockdown of BdB1 significantly increased malathion susceptibility. Furthermore, heterologous expression along with cytotoxicity assay revealed BdB1 could probably have the function of malathion detoxification.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Bactrocera dorsalis, carboxylesterase, Metabolic resistance, Malathion, RNAi, Heterologous expression, ORGANOPHOSPHATE RESISTANCE, INSECTICIDE RESISTANCE, DROSOPHILA-MELANOGASTER, METABOLIC RESISTANCE, GENE-EXPRESSION, LUCILIA-CUPRINA, ESTERASE, FLY, DIPTERA, AMPLIFICATION
journal title
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
Comp. Biochem. Physiol. C-Toxicol. Pharmacol.
volume
200
pages
1 - 8
Web of Science type
Article
Web of Science id
000411419200001
ISSN
1532-0456
DOI
10.1016/j.cbpc.2017.07.001
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8528345
handle
http://hdl.handle.net/1854/LU-8528345
date created
2017-08-07 14:20:06
date last changed
2017-10-18 12:18:28
@article{8528345,
  abstract     = {There are many evidences that insect carboxylesterase possess important physiological roles in xenobiotic metabolism and are implicated in the detoxification of organophosphate (OP) insecticides. Despite the ongoing resistance development in the oriental fruit fly, Bactrocera dorsalis (Hendel), the molecular basis of carboxylesterase and its ability to confer OP resistance remain largely obscure. This study was initiated to provide a better understanding of carboxylesterase-mediated resistance mechanism in a tephritid pest fly. Here, we narrow this research gap by demonstrating a well-conserved esterase B1 gene, BdB1, mediates malathion resistance development via gene upregulation with the use of a laboratory selected malathion-resistant strain (MR) of B. dorsalis. No sequence mutation of BdB1 was detected between MR and the susceptible strain (MS) of B. dorsalis. BdB1 is predominantly expressed in the midgut, a key insect tissue for detoxification. As compared with transcripts in MS, BdB1 was significantly more abundant in multiple tissues in the MR. RNA interference (RNAi)-mediated knockdown of BdB1 significantly increased malathion susceptibility. Furthermore, heterologous expression along with cytotoxicity assay revealed BdB1 could probably have the function of malathion detoxification.},
  author       = {Wang, Luoluo and Lu, Xue-Ping and Smagghe, Guy and Meng, Li-Wei and Wang, Jin-Jun},
  issn         = {1532-0456},
  journal      = {COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY \& PHARMACOLOGY},
  keyword      = {Bactrocera dorsalis,carboxylesterase,Metabolic resistance,Malathion,RNAi,Heterologous expression,ORGANOPHOSPHATE RESISTANCE,INSECTICIDE RESISTANCE,DROSOPHILA-MELANOGASTER,METABOLIC RESISTANCE,GENE-EXPRESSION,LUCILIA-CUPRINA,ESTERASE,FLY,DIPTERA,AMPLIFICATION},
  language     = {eng},
  pages        = {1--8},
  title        = {Functional characterization of BdB1, a well-conserved carboxylesterase among tephritid fruit flies associated with malathion resistance in Bactrocera dorsalis (Hendel)},
  url          = {http://dx.doi.org/10.1016/j.cbpc.2017.07.001},
  volume       = {200},
  year         = {2017},
}

Chicago
Wang, Luoluo, Xue-Ping Lu, Guy Smagghe, Li-Wei Meng, and Jin-Jun Wang. 2017. “Functional Characterization of BdB1, a Well-conserved Carboxylesterase Among Tephritid Fruit Flies Associated with Malathion Resistance in Bactrocera Dorsalis (Hendel).” Comparative Biochemistry and Physiology C-toxicology & Pharmacology 200: 1–8.
APA
Wang, Luoluo, Lu, X.-P., Smagghe, G., Meng, L.-W., & Wang, J.-J. (2017). Functional characterization of BdB1, a well-conserved carboxylesterase among tephritid fruit flies associated with malathion resistance in Bactrocera dorsalis (Hendel). COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, 200, 1–8.
Vancouver
1.
Wang L, Lu X-P, Smagghe G, Meng L-W, Wang J-J. Functional characterization of BdB1, a well-conserved carboxylesterase among tephritid fruit flies associated with malathion resistance in Bactrocera dorsalis (Hendel). COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY. 2017;200:1–8.
MLA
Wang, Luoluo, Xue-Ping Lu, Guy Smagghe, et al. “Functional Characterization of BdB1, a Well-conserved Carboxylesterase Among Tephritid Fruit Flies Associated with Malathion Resistance in Bactrocera Dorsalis (Hendel).” COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY 200 (2017): 1–8. Print.