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Imaging the 3D trace metal and metalloid distribution in mature wheat and rye grains via laser ablation-ICP-mass spectrometry and micro-X-ray fluorescence spectrometry

Stijn Van Malderen UGent, Brecht Laforce UGent, Thibaut Van Acker UGent, Laszlo Vincze UGent and Frank Vanhaecke UGent (2017) JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY. 32(2). p.289-298
abstract
Toxic trace metals and metalloids in human nutrient sources pose a severe health risk, and the processes governing metal accumulation should hence be well understood. In this work, the spatial distribution of toxic trace metals/metalloids and micronutrients (Cr, Mn, Ni, Cu, Zn, As, Cd, Hg and Pb) in mature wheat (Triticum aestivum L.) and rye (Secale cereale L.) grains at typical exposure levels was visualized and quantified via laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) by serial sectioning. The bulk concentrations of these elements were also determined by pneumatic nebulisation-ICP-MS. Furthermore, longitudinal sections were scanned using m-X-ray fluorescence spectrometry to confirm the major element distribution. Serial sectioning in this study was realized via a polishing strategy. Although the methodology is time-consuming and laborious, it enables to access 3D information for samples which cannot be sectioned using a microtome on a depth scale that would otherwise be inaccessible by a laser probe. In the elemental images, strong local enrichment patterns for Mn and Zn are apparent in the aleurone layer/seed coat, vascular tissue of the crease, and embryonic tissue, whereas Cr, As, Cd and Pb have been mainly accumulated in the grain endosperm as a result of different transport and storage dynamics.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ARSENIC-PHYTOCHELATIN COMPLEXES, ORYZA-SATIVA L., HIGH-RESOLUTION, PLANT, UREASES, RICE GRAINS, ZINC, IRON, TRANSPORT, ELEMENTS, MS
journal title
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
J. Anal. At. Spectrom.
volume
32
issue
2
pages
289 - 298
Web of Science type
Article
Web of Science id
000395529800005
ISSN
0267-9477
1364-5544
DOI
10.1039/c6ja00357e
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8528231
handle
http://hdl.handle.net/1854/LU-8528231
date created
2017-08-04 14:40:38
date last changed
2017-09-18 14:23:53
@article{8528231,
  abstract     = {Toxic trace metals and metalloids in human nutrient sources pose a severe health risk, and the processes governing metal accumulation should hence be well understood. In this work, the spatial distribution of toxic trace metals/metalloids and micronutrients (Cr, Mn, Ni, Cu, Zn, As, Cd, Hg and Pb) in mature wheat (Triticum aestivum L.) and rye (Secale cereale L.) grains at typical exposure levels was visualized and quantified via laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) by serial sectioning. The bulk concentrations of these elements were also determined by pneumatic nebulisation-ICP-MS. Furthermore, longitudinal sections were scanned using m-X-ray fluorescence spectrometry to confirm the major element distribution. Serial sectioning in this study was realized via a polishing strategy. Although the methodology is time-consuming and laborious, it enables to access 3D information for samples which cannot be sectioned using a microtome on a depth scale that would otherwise be inaccessible by a laser probe. In the elemental images, strong local enrichment patterns for Mn and Zn are apparent in the aleurone layer/seed coat, vascular tissue of the crease, and embryonic tissue, whereas Cr, As, Cd and Pb have been mainly accumulated in the grain endosperm as a result of different transport and storage dynamics.},
  author       = {Van Malderen, Stijn and Laforce, Brecht and Van Acker, Thibaut and Vincze, Laszlo and Vanhaecke, Frank},
  issn         = {0267-9477},
  journal      = {JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY},
  keyword      = {ARSENIC-PHYTOCHELATIN COMPLEXES,ORYZA-SATIVA L.,HIGH-RESOLUTION,PLANT,UREASES,RICE GRAINS,ZINC,IRON,TRANSPORT,ELEMENTS,MS},
  language     = {eng},
  number       = {2},
  pages        = {289--298},
  title        = {Imaging the 3D trace metal and metalloid distribution in mature wheat and rye grains via laser ablation-ICP-mass spectrometry and micro-X-ray fluorescence spectrometry},
  url          = {http://dx.doi.org/10.1039/c6ja00357e},
  volume       = {32},
  year         = {2017},
}

Chicago
Van Malderen, Stijn, Brecht Laforce, Thibaut Van Acker, Laszlo Vincze, and Frank Vanhaecke. 2017. “Imaging the 3D Trace Metal and Metalloid Distribution in Mature Wheat and Rye Grains via Laser ablation-ICP-mass Spectrometry and micro-X-ray Fluorescence Spectrometry.” Journal of Analytical Atomic Spectrometry 32 (2): 289–298.
APA
Van Malderen, Stijn, Laforce, B., Van Acker, T., Vincze, L., & Vanhaecke, F. (2017). Imaging the 3D trace metal and metalloid distribution in mature wheat and rye grains via laser ablation-ICP-mass spectrometry and micro-X-ray fluorescence spectrometry. JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY, 32(2), 289–298.
Vancouver
1.
Van Malderen S, Laforce B, Van Acker T, Vincze L, Vanhaecke F. Imaging the 3D trace metal and metalloid distribution in mature wheat and rye grains via laser ablation-ICP-mass spectrometry and micro-X-ray fluorescence spectrometry. JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY. 2017;32(2):289–98.
MLA
Van Malderen, Stijn, Brecht Laforce, Thibaut Van Acker, et al. “Imaging the 3D Trace Metal and Metalloid Distribution in Mature Wheat and Rye Grains via Laser ablation-ICP-mass Spectrometry and micro-X-ray Fluorescence Spectrometry.” JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY 32.2 (2017): 289–298. Print.