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Isolation of Vibrionaceae from wild blue mussel (Mytilus edulis) adults and their impact on blue mussel larviculture

Mieke Eggermont UGent, Peter Bossier UGent, Gde Sasmita Julyantoro Pande, Vyshal Delahaut, Ali Md Rayhan, Nipa Gupta, Shikder Saiful Islam, Elsie Yumo, Nancy Nevejan UGent, Patrick Sorgeloos UGent, et al. (2017) FEMS MICROBIOLOGY ECOLOGY. 93(4).
abstract
The blue mussel (Mytilus edulis) is known as a robust bivalve species, although its larviculture appears to be highly susceptible to diseases. In this study, we isolated 17 strains from induced mortality events in healthy wild-caught blue mussel adults and demonstrated that they caused between 17% and 98% mortality in blue mussel larvae in a newly developed, highly controlled immersion challenge test model. Eight of the isolates belong to the Splendidus clade of vibrios, while the other isolates belong to the genus Photobacterium. The genomes of the most virulent Vibrio isolate and the most virulent Photobacterium isolate were sequenced and contained several genes encoding factors that have previously been linked to virulence towards bivalves. In vitro tests confirmed that all 17 isolates were positive for these virulence factors. The sequenced genomes also contained a remarkably high number of multidrug resistance genes. We therefore assessed the sensitivity of all isolates to a broad range of antibiotics and found that there were indeed many strong positive correlations between the sensitivities of the isolates to different antibiotics. Our data provide an ecological insight into mass mortality in blue mussels as they indicate that wild mussels contain a reservoir of pathogenic bacteria.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
bivalve, vibriosis, Photobacterium, Splendidus, aquaculture, immersion challenge, OYSTER CRASSOSTREA-GIGAS, ARGOPECTEN-PURPURATUS LAMARCK, SP NOV., MARINE ORGANISMS, INNATE IMMUNITY, PHOTOBACTERIUM, LARVAE, BACTERIA, HATCHERY, SPLENDIDUS
journal title
FEMS MICROBIOLOGY ECOLOGY
FEMS Microbiol. Ecol.
volume
93
issue
4
article number
fix039
pages
11 pages
Web of Science type
Article
Web of Science id
000405586200019
ISSN
0168-6496
1574-6941
DOI
10.1093/femsec/fix039
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8528172
handle
http://hdl.handle.net/1854/LU-8528172
date created
2017-08-04 09:11:46
date last changed
2017-09-07 09:48:09
@article{8528172,
  abstract     = {The blue mussel (Mytilus edulis) is known as a robust bivalve species, although its larviculture appears to be highly susceptible to diseases. In this study, we isolated 17 strains from induced mortality events in healthy wild-caught blue mussel adults and demonstrated that they caused between 17\% and 98\% mortality in blue mussel larvae in a newly developed, highly controlled immersion challenge test model. Eight of the isolates belong to the Splendidus clade of vibrios, while the other isolates belong to the genus Photobacterium. The genomes of the most virulent Vibrio isolate and the most virulent Photobacterium isolate were sequenced and contained several genes encoding factors that have previously been linked to virulence towards bivalves. In vitro tests confirmed that all 17 isolates were positive for these virulence factors. The sequenced genomes also contained a remarkably high number of multidrug resistance genes. We therefore assessed the sensitivity of all isolates to a broad range of antibiotics and found that there were indeed many strong positive correlations between the sensitivities of the isolates to different antibiotics. Our data provide an ecological insight into mass mortality in blue mussels as they indicate that wild mussels contain a reservoir of pathogenic bacteria.},
  articleno    = {fix039},
  author       = {Eggermont, Mieke and Bossier, Peter and Pande, Gde Sasmita Julyantoro and Delahaut, Vyshal and Rayhan, Ali Md and Gupta, Nipa and Islam, Shikder Saiful and Yumo, Elsie and Nevejan, Nancy and Sorgeloos, Patrick and Gomez-Gil, Bruno and Defoirdt, Tom},
  issn         = {0168-6496},
  journal      = {FEMS MICROBIOLOGY ECOLOGY},
  keyword      = {bivalve,vibriosis,Photobacterium,Splendidus,aquaculture,immersion challenge,OYSTER CRASSOSTREA-GIGAS,ARGOPECTEN-PURPURATUS LAMARCK,SP NOV.,MARINE ORGANISMS,INNATE IMMUNITY,PHOTOBACTERIUM,LARVAE,BACTERIA,HATCHERY,SPLENDIDUS},
  language     = {eng},
  number       = {4},
  pages        = {11},
  title        = {Isolation of Vibrionaceae from wild blue mussel (Mytilus edulis) adults and their impact on blue mussel larviculture},
  url          = {http://dx.doi.org/10.1093/femsec/fix039},
  volume       = {93},
  year         = {2017},
}

Chicago
Eggermont, Mieke, Peter Bossier, Gde Sasmita Julyantoro Pande, Vyshal Delahaut, Ali Md Rayhan, Nipa Gupta, Shikder Saiful Islam, et al. 2017. “Isolation of Vibrionaceae from Wild Blue Mussel (Mytilus Edulis) Adults and Their Impact on Blue Mussel Larviculture.” Fems Microbiology Ecology 93 (4).
APA
Eggermont, Mieke, Bossier, P., Pande, G. S. J., Delahaut, V., Rayhan, A. M., Gupta, N., Islam, S. S., et al. (2017). Isolation of Vibrionaceae from wild blue mussel (Mytilus edulis) adults and their impact on blue mussel larviculture. FEMS MICROBIOLOGY ECOLOGY, 93(4).
Vancouver
1.
Eggermont M, Bossier P, Pande GSJ, Delahaut V, Rayhan AM, Gupta N, et al. Isolation of Vibrionaceae from wild blue mussel (Mytilus edulis) adults and their impact on blue mussel larviculture. FEMS MICROBIOLOGY ECOLOGY. 2017;93(4).
MLA
Eggermont, Mieke, Peter Bossier, Gde Sasmita Julyantoro Pande, et al. “Isolation of Vibrionaceae from Wild Blue Mussel (Mytilus Edulis) Adults and Their Impact on Blue Mussel Larviculture.” FEMS MICROBIOLOGY ECOLOGY 93.4 (2017): n. pag. Print.