Advanced search
1 file | 1.26 MB

Functionalization of gold nanoparticles with nanobodies through physical adsorption

(2017) ANALYTICAL METHODS. 9(23). p.3430-3440
Author
Organization
Abstract
Nanobodies (Nbs) are single-domain antibodies with highly desirable properties employed as versatile tools in structural biology, biochemistry and biomedical sciences. Nbs also have a significant potential for the development of highly specific and user-friendly diagnostic assays. These immuno-assays often employ gold nanoparticles (AuNPs) as a detection label. Despite great advances in Nb technology and the immuno-sensor field, the functionalization of AuNPs with Nbs mostly remains unchartered territory. In this paper, we present a study of the functionalization of AuNPs with Nbs through physical adsorption. We show that Nb pI, solution pH, additives and protein engineering strategies have profound effects on the generation of stable Nb-AuNPs. Our results further demonstrate that physical adsorption can be used to produce functional Nb-AuNPs that could be employed in a wide array of applications.
Keywords
DOMAIN ANTIBODY-FRAGMENTS, COLLOIDAL STABILITY, IMMUNOSENSORS, PROTEINS, SURFACES, BINDING, SIZE, BIOCONJUGATION, IMMOBILIZATION, AGGREGATION

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 1.26 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Goossens, Julie, Hla Sein, Shaohong Lu, Magdalena Radwanska, Serge Muyldermans, Yann G-J Sterckx, and Stefan Magez. 2017. “Functionalization of Gold Nanoparticles with Nanobodies Through Physical Adsorption.” Analytical Methods 9 (23): 3430–3440.
APA
Goossens, Julie, Sein, H., Lu, S., Radwanska, M., Muyldermans, S., Sterckx, Y. G.-J., & Magez, S. (2017). Functionalization of gold nanoparticles with nanobodies through physical adsorption. ANALYTICAL METHODS, 9(23), 3430–3440.
Vancouver
1.
Goossens J, Sein H, Lu S, Radwanska M, Muyldermans S, Sterckx YG-J, et al. Functionalization of gold nanoparticles with nanobodies through physical adsorption. ANALYTICAL METHODS. 2017;9(23):3430–40.
MLA
Goossens, Julie, Hla Sein, Shaohong Lu, et al. “Functionalization of Gold Nanoparticles with Nanobodies Through Physical Adsorption.” ANALYTICAL METHODS 9.23 (2017): 3430–3440. Print.
@article{8528052,
  abstract     = {Nanobodies (Nbs) are single-domain antibodies with highly desirable properties employed as versatile tools in structural biology, biochemistry and biomedical sciences. Nbs also have a significant potential for the development of highly specific and user-friendly diagnostic assays. These immuno-assays often employ gold nanoparticles (AuNPs) as a detection label. Despite great advances in Nb technology and the immuno-sensor field, the functionalization of AuNPs with Nbs mostly remains unchartered territory. In this paper, we present a study of the functionalization of AuNPs with Nbs through physical adsorption. We show that Nb pI, solution pH, additives and protein engineering strategies have profound effects on the generation of stable Nb-AuNPs. Our results further demonstrate that physical adsorption can be used to produce functional Nb-AuNPs that could be employed in a wide array of applications.},
  author       = {Goossens, Julie and Sein, Hla and Lu, Shaohong and Radwanska, Magdalena and Muyldermans, Serge and Sterckx, Yann G-J and Magez, Stefan},
  issn         = {1759-9660},
  journal      = {ANALYTICAL METHODS},
  keyword      = {DOMAIN ANTIBODY-FRAGMENTS,COLLOIDAL STABILITY,IMMUNOSENSORS,PROTEINS,SURFACES,BINDING,SIZE,BIOCONJUGATION,IMMOBILIZATION,AGGREGATION},
  language     = {eng},
  number       = {23},
  pages        = {3430--3440},
  title        = {Functionalization of gold nanoparticles with nanobodies through physical adsorption},
  url          = {http://dx.doi.org/10.1039/c7ay00854f},
  volume       = {9},
  year         = {2017},
}

Altmetric
View in Altmetric
Web of Science
Times cited: