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Cross-linking furan-modified kisspeptin-10 to the KISS receptor

(2017) ACS CHEMICAL BIOLOGY. 12(8). p.2191-2200
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Abstract
Chemical cross-linking is well-established for investigating protein-protein interactions. Traditionally, photo cross-linking is used but is associated with problems of selectivity and UV toxicity in a biological context. We here describe, with live cells and under normal growth conditions, selective cross-linking of a furan-modified peptide ligand to its membrane-presented receptor with zero toxicity, high efficiency, and spatio-specificity. Furan-modified kisspeptin-10 is covalently coupled to its glycosylated membrane receptor, GPR54(KISS1R). This newly expands the applicability of furan-mediated cross-linking not only to proteinprotein cross-linking but also to cross-linking in situ. Moreover, in our earlier reports on nucleic acid interstrand cross-linking, furan activation required external triggers of oxidation (via addition of N-bromo succinimide or singlet oxygen). In contrast, we here show, for multiple cell lines, the spontaneous endogenous oxidation of the furan moiety with concurrent selective cross-link formation. We propose that reactive oxygen species produced by NADPH oxidase (NOX) enzymes form the cellular source establishing furan oxidation.
Keywords
PROTEIN-COUPLED RECEPTOR, MASS-SPECTROMETRY, REACTIVE OXYGEN, BIOORTHOGONAL CHEMISTRY, NOX ENZYMES, INTERACTING PROTEINS, PHOTOAFFINITY PROBES, MAMMALIAN-CELLS, NADPH OXIDASE, LIVING CELLS

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Chicago
Vannecke, Willem, Christophe Ampe, Marleen Van Troys, Massimiliano Beltramo, and Annemieke Madder. 2017. “Cross-linking Furan-modified Kisspeptin-10 to the KISS Receptor.” Acs Chemical Biology 12 (8): 2191–2200.
APA
Vannecke, W., Ampe, C., Van Troys, M., Beltramo, M., & Madder, A. (2017). Cross-linking furan-modified kisspeptin-10 to the KISS receptor. ACS CHEMICAL BIOLOGY, 12(8), 2191–2200.
Vancouver
1.
Vannecke W, Ampe C, Van Troys M, Beltramo M, Madder A. Cross-linking furan-modified kisspeptin-10 to the KISS receptor. ACS CHEMICAL BIOLOGY. 2017;12(8):2191–200.
MLA
Vannecke, Willem, Christophe Ampe, Marleen Van Troys, et al. “Cross-linking Furan-modified Kisspeptin-10 to the KISS Receptor.” ACS CHEMICAL BIOLOGY 12.8 (2017): 2191–2200. Print.
@article{8527538,
  abstract     = {Chemical cross-linking is well-established for investigating protein-protein interactions. Traditionally, photo cross-linking is used but is associated with problems of selectivity and UV toxicity in a biological context. We here describe, with live cells and under normal growth conditions, selective cross-linking of a furan-modified peptide ligand to its membrane-presented receptor with zero toxicity, high efficiency, and spatio-specificity. Furan-modified kisspeptin-10 is covalently coupled to its glycosylated membrane receptor, GPR54(KISS1R). This newly expands the applicability of furan-mediated cross-linking not only to proteinprotein cross-linking but also to cross-linking in situ. Moreover, in our earlier reports on nucleic acid interstrand cross-linking, furan activation required external triggers of oxidation (via addition of N-bromo succinimide or singlet oxygen). In contrast, we here show, for multiple cell lines, the spontaneous endogenous oxidation of the furan moiety with concurrent selective cross-link formation. We propose that reactive oxygen species produced by NADPH oxidase (NOX) enzymes form the cellular source establishing furan oxidation.},
  author       = {Vannecke, Willem and Ampe, Christophe and Van Troys, Marleen and Beltramo, Massimiliano and Madder, Annemieke},
  issn         = {1554-8929},
  journal      = {ACS CHEMICAL BIOLOGY},
  keyword      = {PROTEIN-COUPLED RECEPTOR,MASS-SPECTROMETRY,REACTIVE OXYGEN,BIOORTHOGONAL CHEMISTRY,NOX ENZYMES,INTERACTING PROTEINS,PHOTOAFFINITY PROBES,MAMMALIAN-CELLS,NADPH OXIDASE,LIVING CELLS},
  language     = {eng},
  number       = {8},
  pages        = {2191--2200},
  title        = {Cross-linking furan-modified kisspeptin-10 to the KISS receptor},
  url          = {http://dx.doi.org/10.1021/acschembio.7b00396},
  volume       = {12},
  year         = {2017},
}

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