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Protocols for the delivery of small molecules to the two-spotted spider mite, Tetranychus urticae

Takeshi Suzuki, María Urizarna España, Maria Andreia Nunes, Vladimir Zhurov, Wannes Dermauw UGent, Masahiro Osakabe, Thomas Van Leeuwen UGent, Miodrag Grbic and Vojislava Grbic (2017) PLOS ONE. 12(7).
abstract
The two-spotted spider mite, Tetranychus urticae, is a chelicerate herbivore with an extremely wide host range and an extraordinary ability to develop pesticide resistance. Due to its responsiveness to natural and synthetic xenobiotics, the spider mite is becoming a prime pest herbivore model for studies of the evolution of host range, plant-herbivore interactions and mechanisms of xenobiotic resistance. The spider mite genome has been sequenced and its transcriptional responses to developmental and various biotic and abiotic cues have been documented. However, to identify biological and evolutionary roles of T. urticae genes and proteins, it is necessary to develop methods for the efficient manipulation of mite gene function or protein activity. Here, we describe protocols developed for the delivery of small molecules into spider mites. Starting with mite maintenance and the preparation of the experimental mite populations of developmentally synchronized larvae and adults, we describe 3 methods for delivery of small molecules including artificial diet, leaf coating, and soaking. The presented results define critical steps in these methods and demonstrate that they can successfully deliver tracer dyes into mites. Described protocols provide guidelines for high-throughput setups for delivery of experimental compounds that could be used in reverse genetics platforms to modulate gene expression or protein activity, or for screens focused on discovery of new molecules for mite control. In addition, described protocols could be adapted for other Tetranychidae and related species of economic importance such as Varroa, dust and poultry mites.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
GREEN PEACH APHID, RNA INTERFERENCE, INDOLE GLUCOSINOLATE, BEMISIA-TABACI, HONEY-BEE, GENE, DSRNA, DIET, IMPROVEMENT, ELEGANS
journal title
PLOS ONE
PLoS One
volume
12
issue
7
article number
e0180658
pages
23 pages
Web of Science type
Article
Web of Science id
000405464100088
ISSN
1932-6203
DOI
10.1371/journal.pone.0180658
language
English
UGent publication?
yes
classification
A1
additional info
the first two authors contributed equally to this work
copyright statement
I have retained and own the full copyright for this publication
id
8527519
handle
http://hdl.handle.net/1854/LU-8527519
date created
2017-07-24 13:31:14
date last changed
2017-10-05 13:10:16
@article{8527519,
  abstract     = {The two-spotted spider mite, Tetranychus urticae, is a chelicerate herbivore with an extremely wide host range and an extraordinary ability to develop pesticide resistance. Due to its responsiveness to natural and synthetic xenobiotics, the spider mite is becoming a prime pest herbivore model for studies of the evolution of host range, plant-herbivore interactions and mechanisms of xenobiotic resistance. The spider mite genome has been sequenced and its transcriptional responses to developmental and various biotic and abiotic cues have been documented. However, to identify biological and evolutionary roles of T. urticae genes and proteins, it is necessary to develop methods for the efficient manipulation of mite gene function or protein activity. Here, we describe protocols developed for the delivery of small molecules into spider mites. Starting with mite maintenance and the preparation of the experimental mite populations of developmentally synchronized larvae and adults, we describe 3 methods for delivery of small molecules including artificial diet, leaf coating, and soaking. The presented results define critical steps in these methods and demonstrate that they can successfully deliver tracer dyes into mites. Described protocols provide guidelines for high-throughput setups for delivery of experimental compounds that could be used in reverse genetics platforms to modulate gene expression or protein activity, or for screens focused on discovery of new molecules for mite control. In addition, described protocols could be adapted for other Tetranychidae and related species of economic importance such as Varroa, dust and poultry mites.},
  articleno    = {e0180658},
  author       = {Suzuki, Takeshi and Espa{\~n}a, Mar{\'i}a Urizarna and Nunes, Maria Andreia and Zhurov, Vladimir and Dermauw, Wannes and Osakabe, Masahiro and Van Leeuwen, Thomas and Grbic, Miodrag and Grbic, Vojislava},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {GREEN PEACH APHID,RNA INTERFERENCE,INDOLE GLUCOSINOLATE,BEMISIA-TABACI,HONEY-BEE,GENE,DSRNA,DIET,IMPROVEMENT,ELEGANS},
  language     = {eng},
  number       = {7},
  pages        = {23},
  title        = {Protocols for the delivery of small molecules to the two-spotted spider mite, Tetranychus urticae},
  url          = {http://dx.doi.org/10.1371/journal.pone.0180658},
  volume       = {12},
  year         = {2017},
}

Chicago
Suzuki, Takeshi, María Urizarna España, Maria Andreia Nunes, Vladimir Zhurov, Wannes Dermauw, Masahiro Osakabe, Thomas Van Leeuwen, Miodrag Grbic, and Vojislava Grbic. 2017. “Protocols for the Delivery of Small Molecules to the Two-spotted Spider Mite, Tetranychus Urticae.” Plos One 12 (7).
APA
Suzuki, Takeshi, España, M. U., Nunes, M. A., Zhurov, V., Dermauw, W., Osakabe, M., Van Leeuwen, T., et al. (2017). Protocols for the delivery of small molecules to the two-spotted spider mite, Tetranychus urticae. PLOS ONE, 12(7).
Vancouver
1.
Suzuki T, España MU, Nunes MA, Zhurov V, Dermauw W, Osakabe M, et al. Protocols for the delivery of small molecules to the two-spotted spider mite, Tetranychus urticae. PLOS ONE. 2017;12(7).
MLA
Suzuki, Takeshi, María Urizarna España, Maria Andreia Nunes, et al. “Protocols for the Delivery of Small Molecules to the Two-spotted Spider Mite, Tetranychus Urticae.” PLOS ONE 12.7 (2017): n. pag. Print.