Ghent University Academic Bibliography

Advanced

Tetranychus urticae mites do not mount an induced immune response against bacteria

Gonçalo Santos-Matos, Nicky Wybouw UGent, Nelson E Martins, Flore Zélé, Maria Riga, Alexandre B Leitão, John Vontas, Miodrag Grbić, Thomas Van Leeuwen UGent, Sara Magalhães, et al. (2017) PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES. 284(1856).
abstract
The genome of the spider mite Tetranychus urticae, a herbivore, is missing important elements of the canonical Drosophila immune pathways necessary to fight bacterial infections. However, it is not known whether spider mites can mount an immune response and survive bacterial infection. In other chelicerates, bacterial infection elicits a response mediated by immune effectors leading to the survival of infected organisms. In T. urticae, infection by either Escherichia coli or Bacillus megaterium did not elicit a response as assessed through genome-wide transcriptomic analysis. In line with this, spider mites died within days even upon injection with low doses of bacteria that are non-pathogenic to Drosophila. Moreover, bacterial populations grew exponentially inside the infected spider mites. By contrast, Sancassania berlesei, a litter-dwelling mite, controlled bacterial proliferation and resisted infections with both Gram-negative and Gram-positive bacteria lethal to T. urticae. This differential mortality between mite species was absent when mites were infected with heat-killed bacteria. Also, we found that spider mites harbour in their gut 1000-fold less bacteria than S. berlesei. We show that T. urticae has lost the capacity to mount an induced immune response against bacteria, in contrast to other mites and chelicerates but similarly to the phloem feeding aphid Acyrthosiphon pisum. Hence, our results reinforce the putative evolutionary link between ecological conditions regarding exposure to bacteria and the architecture of the immune response.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
host-parasite interactions, Tetranychus urticae, Sancassania berlesei, microbiota, immunity, ACYRTHOSIPHON-PISUM, HOST-DEFENSE, DROSOPHILA-MELANOGASTER, ANTIBACTERIAL DEFENSE, INNATE IMMUNITY, PEA APHID, REVEALS, GUT, INFECTION, PATHWAYS
journal title
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
Proc. R. Soc. B-Biol. Sci.
volume
284
issue
1856
article number
20170401
pages
8 pages
Web of Science type
Article
Web of Science id
000405955300011
ISSN
0962-8452
1471-2954
DOI
10.1098/rspb.2017.0401
language
English
UGent publication?
yes
classification
A1
additional info
the first two authors contributed equally to this study; the last two authors are joint last authors
copyright statement
Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
id
8527506
handle
http://hdl.handle.net/1854/LU-8527506
date created
2017-07-24 12:43:30
date last changed
2017-10-11 14:22:34
@article{8527506,
  abstract     = {The genome of the spider mite Tetranychus urticae, a herbivore, is missing important elements of the canonical Drosophila immune pathways necessary to fight bacterial infections. However, it is not known whether spider mites can mount an immune response and survive bacterial infection. In other chelicerates, bacterial infection elicits a response mediated by immune effectors leading to the survival of infected organisms. In T. urticae, infection by either Escherichia coli or Bacillus megaterium did not elicit a response as assessed through genome-wide transcriptomic analysis. In line with this, spider mites died within days even upon injection with low doses of bacteria that are non-pathogenic to Drosophila. Moreover, bacterial populations grew exponentially inside the infected spider mites. By contrast, Sancassania berlesei, a litter-dwelling mite, controlled bacterial proliferation and resisted infections with both Gram-negative and Gram-positive bacteria lethal to T. urticae. This differential mortality between mite species was absent when mites were infected with heat-killed bacteria. Also, we found that spider mites harbour in their gut 1000-fold less bacteria than S. berlesei. We show that T. urticae has lost the capacity to mount an induced immune response against bacteria, in contrast to other mites and chelicerates but similarly to the phloem feeding aphid Acyrthosiphon pisum. Hence, our results reinforce the putative evolutionary link between ecological conditions regarding exposure to bacteria and the architecture of the immune response.},
  articleno    = {20170401},
  author       = {Santos-Matos, Gon\c{c}alo and Wybouw, Nicky and Martins, Nelson E and Z{\'e}l{\'e}, Flore and Riga, Maria and Leit{\~a}o, Alexandre B and Vontas, John and Grbi\'{c}, Miodrag and Van Leeuwen, Thomas and Magalh{\~a}es, Sara and Sucena, {\'E}lio},
  issn         = {0962-8452},
  journal      = {PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES},
  keyword      = {host-parasite interactions,Tetranychus urticae,Sancassania berlesei,microbiota,immunity,ACYRTHOSIPHON-PISUM,HOST-DEFENSE,DROSOPHILA-MELANOGASTER,ANTIBACTERIAL DEFENSE,INNATE IMMUNITY,PEA APHID,REVEALS,GUT,INFECTION,PATHWAYS},
  language     = {eng},
  number       = {1856},
  pages        = {8},
  title        = {Tetranychus urticae mites do not mount an induced immune response against bacteria},
  url          = {http://dx.doi.org/10.1098/rspb.2017.0401},
  volume       = {284},
  year         = {2017},
}

Chicago
Santos-Matos, Gonçalo, Nicky Wybouw, Nelson E Martins, Flore Zélé, Maria Riga, Alexandre B Leitão, John Vontas, et al. 2017. “Tetranychus Urticae Mites Do Not Mount an Induced Immune Response Against Bacteria.” Proceedings of the Royal Society B-biological Sciences 284 (1856).
APA
Santos-Matos, G., Wybouw, N., Martins, N. E., Zélé, F., Riga, M., Leitão, A. B., Vontas, J., et al. (2017). Tetranychus urticae mites do not mount an induced immune response against bacteria. PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 284(1856).
Vancouver
1.
Santos-Matos G, Wybouw N, Martins NE, Zélé F, Riga M, Leitão AB, et al. Tetranychus urticae mites do not mount an induced immune response against bacteria. PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES. 2017;284(1856).
MLA
Santos-Matos, Gonçalo, Nicky Wybouw, Nelson E Martins, et al. “Tetranychus Urticae Mites Do Not Mount an Induced Immune Response Against Bacteria.” PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES 284.1856 (2017): n. pag. Print.