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Age and anti-Müllerian hormone levels predict the success of in vitro maturation of cat oocytes

Féline Snoeck (UGent) , Steven Sarrazin (UGent) , Eline Wydooghe (UGent) and Ann Van Soom (UGent)
(2017) REPRODUCTION IN DOMESTIC ANIMALS. 52(suppl. 2). p.98-102
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Abstract
Contents Up to date, in vitro maturation (IVM) rates of oocytes are highly variable between individual cats. This study was carried out to investigate the predictive value of age and anti-Mullerian hormone (AMH) concentration in relation to capacity for IVM of cat oocytes. Ovaries were collected from 33 cats, which were divided into three age groups: (i) 0-3months (pre-pubertal); (ii) 3-12months (peripubertal); and (iii) older than 12months (pubertal). The cumulus-oocyte complexes (COCs) were matured and subsequently stained to check nuclear maturation status, and blood was taken for AMH analysis. Increasing age was significantly associated with decreasing AMH levels, and mean AMH levels differed significantly between all age categories: group 1: mean AMH 18.71g/L; group 2: mean AMH 9.27g/L; and group 3: mean AMH 4.13g/L. Moreover, the probability of maturation was more likely in groups 2 and 3 compared to group 1. Between categories 2 and 3, no significant difference in maturation probability was found (p=.31). Finally, the probability of oocyte maturation decreased significantly with increasing AMH levels. In age group 2, oocytes with a higher AMH level were less likely to mature. In age groups 1 and 3, no significant association between the AMH level and the proportion of maturated COC was found. We can conclude that if a higher probability of nuclear maturation is required, it is preferable to use cats with lower AMH levels and older than 3months of age to improve cat IVM.
Keywords
DOMESTIC CAT, OOPLASMIC MATURATION, NUCLEAR MATURATION, COMPETENCE, QUALITY, OVARIES

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Chicago
Snoeck, Féline, Steven Sarrazin, Eline Wydooghe, and Ann Van Soom. 2017. “Age and anti-Müllerian Hormone Levels Predict the Success of in Vitro Maturation of Cat Oocytes.” Reproduction in Domestic Animals 52 (suppl. 2): 98–102.
APA
Snoeck, F., Sarrazin, S., Wydooghe, E., & Van Soom, A. (2017). Age and anti-Müllerian hormone levels predict the success of in vitro maturation of cat oocytes. REPRODUCTION IN DOMESTIC ANIMALS, 52(suppl. 2), 98–102. Presented at the 8th International symposium on Canine and Feline Reproduction (ISCFR) ; 19th Congress of the European Veterinary Society for Small Animal Reproduction (EVSSAR).
Vancouver
1.
Snoeck F, Sarrazin S, Wydooghe E, Van Soom A. Age and anti-Müllerian hormone levels predict the success of in vitro maturation of cat oocytes. REPRODUCTION IN DOMESTIC ANIMALS. 2017;52(suppl. 2):98–102.
MLA
Snoeck, Féline, Steven Sarrazin, Eline Wydooghe, et al. “Age and anti-Müllerian Hormone Levels Predict the Success of in Vitro Maturation of Cat Oocytes.” REPRODUCTION IN DOMESTIC ANIMALS 52.suppl. 2 (2017): 98–102. Print.
@article{8526924,
  abstract     = {Contents Up to date, in vitro maturation (IVM) rates of oocytes are highly variable between individual cats. This study was carried out to investigate the predictive value of age and anti-Mullerian hormone (AMH) concentration in relation to capacity for IVM of cat oocytes. Ovaries were collected from 33 cats, which were divided into three age groups: (i) 0-3months (pre-pubertal); (ii) 3-12months (peripubertal); and (iii) older than 12months (pubertal). The cumulus-oocyte complexes (COCs) were matured and subsequently stained to check nuclear maturation status, and blood was taken for AMH analysis. Increasing age was significantly associated with decreasing AMH levels, and mean AMH levels differed significantly between all age categories: group 1: mean AMH 18.71g/L; group 2: mean AMH 9.27g/L; and group 3: mean AMH 4.13g/L. Moreover, the probability of maturation was more likely in groups 2 and 3 compared to group 1. Between categories 2 and 3, no significant difference in maturation probability was found (p=.31). Finally, the probability of oocyte maturation decreased significantly with increasing AMH levels. In age group 2, oocytes with a higher AMH level were less likely to mature. In age groups 1 and 3, no significant association between the AMH level and the proportion of maturated COC was found. We can conclude that if a higher probability of nuclear maturation is required, it is preferable to use cats with lower AMH levels and older than 3months of age to improve cat IVM.},
  author       = {Snoeck, F{\'e}line and Sarrazin, Steven and Wydooghe, Eline and Van Soom, Ann},
  issn         = {0936-6768},
  journal      = {REPRODUCTION IN DOMESTIC ANIMALS},
  language     = {eng},
  location     = {Paris, France},
  number       = {suppl. 2},
  pages        = {98--102},
  title        = {Age and anti-M{\"u}llerian hormone levels predict the success of in vitro maturation of cat oocytes},
  url          = {http://dx.doi.org/10.1111/rda.12827},
  volume       = {52},
  year         = {2017},
}

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