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The transcription factor bZIP14 regulates the TCA cycle in the diatom Phaeodactylum tricornutum

(2017) EMBO JOURNAL. 36(11). p.1559-1576
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Abstract
Diatoms are amongst the most important marine microalgae in terms of biomass, but little is known concerning the molecular mechanisms that regulate their versatile metabolism. Here, the pennate diatom Phaeodactylum tricornutum was studied at the metabolite and transcriptome level during nitrogen starvation and following imposition of three other stresses that impede growth. The coordinated upregulation of the tricarboxylic acid (TCA) cycle during the nitrogen stress response was the most striking observation. Through co-expression analysis and DNA binding assays, the transcription factor bZIP14 was identified as a regulator of the TCA cycle, also beyond the nitrogen starvation response, namely in diurnal regulation. Accordingly, metabolic and transcriptional shifts were observed upon overexpression of bZIP14 in transformed P. tricornutum cells. Our data indicate that the TCA cycle is a tightly regulated and important hub for carbon reallocation in the diatom cell during nutrient starvation and that bZIP14 is a conserved regulator of this cycle.
Keywords
PROTEIN-BINDING MICROARRAYS, LIPID-ACCUMULATION, THALASSIOSIRA-PSEUDONANA, NITROGEN DEPRIVATION, METABOLIC-RESPONSES, CARBON METABOLISM, LIGHT/DARK CYCLES, GABA SHUNT, CD-HIT, GENOME, bZIP, diurnal, nitrogen, Phaeodactylum tricornutum, tricarboxylic acid, cycle

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Citation

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Chicago
Matthijs, Michiel, Michele Fabris, Toshihiro Obata, Imogen Foubert, Jose Manuel Franco-Zorrilla, Roberto Solano, Alisdair R Fernie, Wim Vyverman, and Alain Goossens. 2017. “The Transcription Factor bZIP14 Regulates the TCA Cycle in the Diatom Phaeodactylum Tricornutum.” Embo Journal 36 (11): 1559–1576.
APA
Matthijs, M., Fabris, M., Obata, T., Foubert, I., Franco-Zorrilla, J. M., Solano, R., Fernie, A. R., et al. (2017). The transcription factor bZIP14 regulates the TCA cycle in the diatom Phaeodactylum tricornutum. EMBO JOURNAL, 36(11), 1559–1576.
Vancouver
1.
Matthijs M, Fabris M, Obata T, Foubert I, Franco-Zorrilla JM, Solano R, et al. The transcription factor bZIP14 regulates the TCA cycle in the diatom Phaeodactylum tricornutum. EMBO JOURNAL. 2017;36(11):1559–76.
MLA
Matthijs, Michiel et al. “The Transcription Factor bZIP14 Regulates the TCA Cycle in the Diatom Phaeodactylum Tricornutum.” EMBO JOURNAL 36.11 (2017): 1559–1576. Print.
@article{8526825,
  abstract     = {Diatoms are amongst the most important marine microalgae in terms of biomass, but little is known concerning the molecular mechanisms that regulate their versatile metabolism. Here, the pennate diatom Phaeodactylum tricornutum was studied at the metabolite and transcriptome level during nitrogen starvation and following imposition of three other stresses that impede growth. The coordinated upregulation of the tricarboxylic acid (TCA) cycle during the nitrogen stress response was the most striking observation. Through co-expression analysis and DNA binding assays, the transcription factor bZIP14 was identified as a regulator of the TCA cycle, also beyond the nitrogen starvation response, namely in diurnal regulation. Accordingly, metabolic and transcriptional shifts were observed upon overexpression of bZIP14 in transformed P. tricornutum cells. Our data indicate that the TCA cycle is a tightly regulated and important hub for carbon reallocation in the diatom cell during nutrient starvation and that bZIP14 is a conserved regulator of this cycle.},
  author       = {Matthijs, Michiel and Fabris, Michele and Obata, Toshihiro and Foubert, Imogen and Franco-Zorrilla, Jose Manuel and Solano, Roberto and Fernie, Alisdair R and Vyverman, Wim and Goossens, Alain},
  issn         = {0261-4189},
  journal      = {EMBO JOURNAL},
  keywords     = {PROTEIN-BINDING MICROARRAYS,LIPID-ACCUMULATION,THALASSIOSIRA-PSEUDONANA,NITROGEN DEPRIVATION,METABOLIC-RESPONSES,CARBON METABOLISM,LIGHT/DARK CYCLES,GABA SHUNT,CD-HIT,GENOME,bZIP,diurnal,nitrogen,Phaeodactylum tricornutum,tricarboxylic acid,cycle},
  language     = {eng},
  number       = {11},
  pages        = {1559--1576},
  title        = {The transcription factor bZIP14 regulates the TCA cycle in the diatom Phaeodactylum tricornutum},
  url          = {http://dx.doi.org/10.15252/embj.201696392},
  volume       = {36},
  year         = {2017},
}

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