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Biocatalytic synthesis of the rare sugar kojibiose : process scale-up and application testing

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Abstract
Cost-efficient (bio)chemical production processes are essential to evaluate the commercial and industrial applications of promising carbohydrates and also are essential to ensure economically viable production processes. Here, the synthesis of the naturally occurring disaccharide kojibiose (2-O-α-d-glucopyranosyl-d-glucopyranoside) was evaluated using different Bifidobacterium adolescentis sucrose phosphorylase variants. Variant L341I\_Q345S was found to efficiently synthesize kojibiose while remaining fully active after 1 week of incubation at 55 °C. Process optimization allowed kojibiose production at the kilogram scale, and simple but efficient downstream processing, using a yeast treatment and crystallization, resulted in more than 3 kg of highly pure crystalline kojibiose (99.8\%). These amounts allowed a deeper characterization of its potential in food applications. It was found to have possible beneficial health effects, including delayed glucose release and potential to trigger SCFA production. Finally, we compared the bulk functionality of highly pure kojibiose to that of sucrose, hereby mapping its potential as a new sweetener in confectionery products.
Keywords
sucrose phosphorylase, kojibiose, bulk functionality, Caco-2 cells, gut microbiota, fermentable sugars, LYSOSOMAL ALPHA-GLUCOSIDASE, PARTICLE-SIZE DISTRIBUTION, IN-VITRO, ENZYMATIC-SYNTHESIS, OLIGOSACCHARIDES, PHOSPHORYLASE, DIGESTION, CHOCOLATE, COLON, FERMENTATION

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Chicago
Beerens, Koen, Karel De Winter, Davy Van de Walle, Charlotte Grootaert, Senem Kamiloglu, Lisa Miclotte, Tom Van de Wiele, John Van Camp, Koen Dewettinck, and Tom Desmet. 2017. “Biocatalytic Synthesis of the Rare Sugar Kojibiose : Process Scale-up and Application Testing.” Journal of Agricultural and Food Chemistry 65 (29): 6030–6041.
APA
Beerens, K., De Winter, K., Van de Walle, D., Grootaert, C., Kamiloglu, S., Miclotte, L., Van de Wiele, T., et al. (2017). Biocatalytic synthesis of the rare sugar kojibiose : process scale-up and application testing. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 65(29), 6030–6041.
Vancouver
1.
Beerens K, De Winter K, Van de Walle D, Grootaert C, Kamiloglu S, Miclotte L, et al. Biocatalytic synthesis of the rare sugar kojibiose : process scale-up and application testing. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 2017;65(29):6030–41.
MLA
Beerens, Koen, Karel De Winter, Davy Van de Walle, et al. “Biocatalytic Synthesis of the Rare Sugar Kojibiose : Process Scale-up and Application Testing.” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 65.29 (2017): 6030–6041. Print.
@article{8526813,
  abstract     = { Cost-efficient (bio)chemical production processes are essential to evaluate the commercial and industrial applications of promising carbohydrates and also are essential to ensure economically viable production processes. Here, the synthesis of the naturally occurring disaccharide kojibiose (2-O-\ensuremath{\alpha}-d-glucopyranosyl-d-glucopyranoside) was evaluated using different Bifidobacterium adolescentis sucrose phosphorylase variants. Variant L341I{\textbackslash}\_Q345S was found to efficiently synthesize kojibiose while remaining fully active after 1 week of incubation at 55 {\textdegree}C. Process optimization allowed kojibiose production at the kilogram scale, and simple but efficient downstream processing, using a yeast treatment and crystallization, resulted in more than 3 kg of highly pure crystalline kojibiose (99.8{\textbackslash}\%). These amounts allowed a deeper characterization of its potential in food applications. It was found to have possible beneficial health effects, including delayed glucose release and potential to trigger SCFA production. Finally, we compared the bulk functionality of highly pure kojibiose to that of sucrose, hereby mapping its potential as a new sweetener in confectionery products. },
  author       = {Beerens, Koen and De Winter, Karel and Van de Walle, Davy and Grootaert, Charlotte and Kamiloglu, Senem and Miclotte, Lisa and Van de Wiele, Tom and Van Camp, John and Dewettinck, Koen and Desmet, Tom},
  issn         = {000406649400021},
  journal      = {JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY},
  keyword      = {sucrose phosphorylase,kojibiose,bulk functionality,Caco-2 cells,gut microbiota,fermentable sugars,LYSOSOMAL ALPHA-GLUCOSIDASE,PARTICLE-SIZE DISTRIBUTION,IN-VITRO,ENZYMATIC-SYNTHESIS,OLIGOSACCHARIDES,PHOSPHORYLASE,DIGESTION,CHOCOLATE,COLON,FERMENTATION},
  language     = {eng},
  number       = {29},
  pages        = {6030--6041},
  title        = {Biocatalytic synthesis of the rare sugar kojibiose : process scale-up and application testing},
  url          = {http://dx.doi.org/10.1021/acs.jafc.7b02258},
  volume       = {65},
  year         = {2017},
}

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