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A Mutation in the bHLH Domain of the SPCH Transcription Factor Uncovers a BR-Dependent Mechanism for Stomatal Development

Alberto de Marcos, Anaxi Houbaert UGent, Magdalena Trivino, Dolores Delgado, Mar Martin-Trillo, Eugenia Russinova UGent, Carmen Fenoll and Montana Mena (2017) PLANT PHYSIOLOGY. 174(2). p.823-842
abstract
The asymmetric cell divisions necessary for stomatal lineage initiation and progression in Arabidopsis (Arabidopsis thaliana) require the function of the basic helix-loop-helix (bHLH) transcription factor SPEECHLESS (SPCH). Mutants lacking SPCH do not produce stomata or lineages. Here, we isolated a new spch-5 allele carrying a point mutation in the bHLH domain that displayed normal growth, but had an extremely low number of sometimes clustered stomata in the leaves, whereas the hypocotyls did not have any stomata. In vivo tracking of leaf epidermal cell divisions, combined with marker lines and genetic analysis, showed that the spch-5 leaf phenotype is dosage dependent and results from the decreased ability to initiate and amplify lineages, defects in asymmetric cell fate allocation, and misorientation of asymmetric division planes. Notably, application of brassinosteroids (BRs) partly rescued the stomatal leaf phenotype of spch-5. Transcriptomic analysis combining spch-5 with BR treatments revealed that the expression of a set of SPCH target genes was restored by BRs. Our results also show that BR-dependent stomata formation and expression of some, but not all, SPCH target genes require the integrity of the bHLH domain of SPCH.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
HELIX-LOOP-HELIX, ASYMMETRIC CELL-DIVISION, TOO-MANY-MOUTHS, ARABIDOPSIS-THALIANA, MEDIATED REGULATION, INTERACTION NETWORK, MICROARRAY DATA, SWISS-MODEL, DNA-BINDING, DIFFERENTIATION
journal title
PLANT PHYSIOLOGY
Plant Physiol.
volume
174
issue
2
pages
20 pages
publisher
Amer Soc Plant Biologists
place of publication
Rockville
Web of Science type
Article
Web of Science id
000403152200031
ISSN
0032-0889
DOI
10.1104/pp.17.00615
language
English
UGent publication?
yes
classification
U
id
8526590
handle
http://hdl.handle.net/1854/LU-8526590
date created
2017-07-06 13:55:16
date last changed
2017-07-06 13:55:16
@article{8526590,
  abstract     = {The asymmetric cell divisions necessary for stomatal lineage initiation and progression in Arabidopsis (Arabidopsis thaliana) require the function of the basic helix-loop-helix (bHLH) transcription factor SPEECHLESS (SPCH). Mutants lacking SPCH do not produce stomata or lineages. Here, we isolated a new spch-5 allele carrying a point mutation in the bHLH domain that displayed normal growth, but had an extremely low number of sometimes clustered stomata in the leaves, whereas the hypocotyls did not have any stomata. In vivo tracking of leaf epidermal cell divisions, combined with marker lines and genetic analysis, showed that the spch-5 leaf phenotype is dosage dependent and results from the decreased ability to initiate and amplify lineages, defects in asymmetric cell fate allocation, and misorientation of asymmetric division planes. Notably, application of brassinosteroids (BRs) partly rescued the stomatal leaf phenotype of spch-5. Transcriptomic analysis combining spch-5 with BR treatments revealed that the expression of a set of SPCH target genes was restored by BRs. Our results also show that BR-dependent stomata formation and expression of some, but not all, SPCH target genes require the integrity of the bHLH domain of SPCH.},
  author       = {de Marcos, Alberto and Houbaert, Anaxi and Trivino, Magdalena and Delgado, Dolores and Martin-Trillo, Mar and Russinova, Eugenia and Fenoll, Carmen and Mena, Montana},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keyword      = {HELIX-LOOP-HELIX,ASYMMETRIC CELL-DIVISION,TOO-MANY-MOUTHS,ARABIDOPSIS-THALIANA,MEDIATED REGULATION,INTERACTION NETWORK,MICROARRAY DATA,SWISS-MODEL,DNA-BINDING,DIFFERENTIATION},
  language     = {eng},
  number       = {2},
  pages        = {823--842},
  publisher    = {Amer Soc Plant Biologists},
  title        = {A Mutation in the bHLH Domain of the SPCH Transcription Factor Uncovers a BR-Dependent Mechanism for Stomatal Development},
  url          = {http://dx.doi.org/10.1104/pp.17.00615},
  volume       = {174},
  year         = {2017},
}

Chicago
de Marcos, Alberto, Anaxi Houbaert, Magdalena Trivino, Dolores Delgado, Mar Martin-Trillo, Eugenia Russinova, Carmen Fenoll, and Montana Mena. 2017. “A Mutation in the bHLH Domain of the SPCH Transcription Factor Uncovers a BR-Dependent Mechanism for Stomatal Development.” Plant Physiology 174 (2): 823–842.
APA
de Marcos, Alberto, Houbaert, A., Trivino, M., Delgado, D., Martin-Trillo, M., Russinova, E., Fenoll, C., et al. (2017). A Mutation in the bHLH Domain of the SPCH Transcription Factor Uncovers a BR-Dependent Mechanism for Stomatal Development. PLANT PHYSIOLOGY, 174(2), 823–842.
Vancouver
1.
de Marcos A, Houbaert A, Trivino M, Delgado D, Martin-Trillo M, Russinova E, et al. A Mutation in the bHLH Domain of the SPCH Transcription Factor Uncovers a BR-Dependent Mechanism for Stomatal Development. PLANT PHYSIOLOGY. Rockville: Amer Soc Plant Biologists; 2017;174(2):823–42.
MLA
de Marcos, Alberto, Anaxi Houbaert, Magdalena Trivino, et al. “A Mutation in the bHLH Domain of the SPCH Transcription Factor Uncovers a BR-Dependent Mechanism for Stomatal Development.” PLANT PHYSIOLOGY 174.2 (2017): 823–842. Print.