Advanced search
1 file | 5.49 MB Add to list

Exploiting cell cycle inhibitor genes of the KRP family to control root-knot nematode induced feeding sites in plants

(2017) PLANT CELL AND ENVIRONMENT. 40(7). p.1174-1188
Author
Organization
Abstract
Cell cycle control in galls provoked by root-knot nematodes involves the activity of inhibitor genes like the Arabidopsis ICK/KRP members. Ectopic KRP1, KRP2 and KRP4 expression resulted in decreased gall size by inhibiting mitotic activity, whereas KRP6 induces mitosis in galls. Herein, we investigate the role of KRP3, KRP5 and KRP7 during gall development and compared their role with previously studied members of this class of cell cycle inhibitors. Overexpression of KRP3 and KRP7 culminated in undersized giant cells, with KRP3(OE) galls presenting peculiar elongated giant cells. Nuclei in KRP3(OE) and KRP5(OE) lines presented a convoluted and apparently connected phenotype. This appearancemay be associated with the punctuated protein nuclear localization driven by specific common motifs. As well, ectopic expression of KRP3(OE) and KRP5(OE) affected nematode development and offspring. Decreased mitotic activity in galls of KRP3(OE) and KRP7(OE) lines led to a reduced gall size which presented distinct shapes-from more elongated like in the KRP3(OE) line to small rounded like in the KRP7(OE) line. Results presented strongly support the idea that induced expression of cell cycle inhibitors such as KRP3 and KRP7 in galls can be envisaged as a conceivable strategy for nematode feeding site control in crop species attacked by phytopathogenic nematodes.
Keywords
DEPENDENT KINASE INHIBITORS, KIP-RELATED PROTEINS, ARABIDOPSIS-THALIANA, MELOIDOGYNE-INCOGNITA, PARASITIC NEMATODES, E3 LIGASES, EXPRESSION, LOCALIZATION, DEGRADATION, NUCLEAR, Meloidogyne incognita, Arabidopsis, giant cells

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 5.49 MB

Citation

Please use this url to cite or link to this publication:

MLA
Coelho, Roberta Ramos et al. “Exploiting Cell Cycle Inhibitor Genes of the KRP Family to Control Root-knot Nematode Induced Feeding Sites in Plants.” PLANT CELL AND ENVIRONMENT 40.7 (2017): 1174–1188. Print.
APA
Coelho, R. R., Vieira, P., Antonino de Souza Jûnior, J. D., Martin-Jimenez, C., De Veylder, L., Cazareth, J., Engler, G., et al. (2017). Exploiting cell cycle inhibitor genes of the KRP family to control root-knot nematode induced feeding sites in plants. PLANT CELL AND ENVIRONMENT, 40(7), 1174–1188.
Chicago author-date
Coelho, Roberta Ramos, Paulo Vieira, José Dijair Antonino de Souza Jûnior, Cristina Martin-Jimenez, Lieven De Veylder, Julie Cazareth, Gilbert Engler, Maria Fatima Grossi-de-Sa, and Janice de Almeida Engler. 2017. “Exploiting Cell Cycle Inhibitor Genes of the KRP Family to Control Root-knot Nematode Induced Feeding Sites in Plants.” Plant Cell and Environment 40 (7): 1174–1188.
Chicago author-date (all authors)
Coelho, Roberta Ramos, Paulo Vieira, José Dijair Antonino de Souza Jûnior, Cristina Martin-Jimenez, Lieven De Veylder, Julie Cazareth, Gilbert Engler, Maria Fatima Grossi-de-Sa, and Janice de Almeida Engler. 2017. “Exploiting Cell Cycle Inhibitor Genes of the KRP Family to Control Root-knot Nematode Induced Feeding Sites in Plants.” Plant Cell and Environment 40 (7): 1174–1188.
Vancouver
1.
Coelho RR, Vieira P, Antonino de Souza Jûnior JD, Martin-Jimenez C, De Veylder L, Cazareth J, et al. Exploiting cell cycle inhibitor genes of the KRP family to control root-knot nematode induced feeding sites in plants. PLANT CELL AND ENVIRONMENT. 2017;40(7):1174–88.
IEEE
[1]
R. R. Coelho et al., “Exploiting cell cycle inhibitor genes of the KRP family to control root-knot nematode induced feeding sites in plants,” PLANT CELL AND ENVIRONMENT, vol. 40, no. 7, pp. 1174–1188, 2017.
@article{8526574,
  abstract     = {Cell cycle control in galls provoked by root-knot nematodes involves the activity of inhibitor genes like the Arabidopsis ICK/KRP members. Ectopic KRP1, KRP2 and KRP4 expression resulted in decreased gall size by inhibiting mitotic activity, whereas KRP6 induces mitosis in galls. Herein, we investigate the role of KRP3, KRP5 and KRP7 during gall development and compared their role with previously studied members of this class of cell cycle inhibitors. Overexpression of KRP3 and KRP7 culminated in undersized giant cells, with KRP3(OE) galls presenting peculiar elongated giant cells. Nuclei in KRP3(OE) and KRP5(OE) lines presented a convoluted and apparently connected phenotype. This appearancemay be associated with the punctuated protein nuclear localization driven by specific common motifs. As well, ectopic expression of KRP3(OE) and KRP5(OE) affected nematode development and offspring. Decreased mitotic activity in galls of KRP3(OE) and KRP7(OE) lines led to a reduced gall size which presented distinct shapes-from more elongated like in the KRP3(OE) line to small rounded like in the KRP7(OE) line. Results presented strongly support the idea that induced expression of cell cycle inhibitors such as KRP3 and KRP7 in galls can be envisaged as a conceivable strategy for nematode feeding site control in crop species attacked by phytopathogenic nematodes.},
  author       = {Coelho, Roberta Ramos and Vieira, Paulo and Antonino de Souza Jûnior, José Dijair and Martin-Jimenez, Cristina and De Veylder, Lieven and Cazareth, Julie and Engler, Gilbert and Grossi-de-Sa, Maria Fatima and Engler, Janice de Almeida},
  issn         = {0140-7791},
  journal      = {PLANT CELL AND ENVIRONMENT},
  keywords     = {DEPENDENT KINASE INHIBITORS,KIP-RELATED PROTEINS,ARABIDOPSIS-THALIANA,MELOIDOGYNE-INCOGNITA,PARASITIC NEMATODES,E3 LIGASES,EXPRESSION,LOCALIZATION,DEGRADATION,NUCLEAR,Meloidogyne incognita,Arabidopsis,giant cells},
  language     = {eng},
  number       = {7},
  pages        = {1174--1188},
  title        = {Exploiting cell cycle inhibitor genes of the KRP family to control root-knot nematode induced feeding sites in plants},
  url          = {http://dx.doi.org/10.1111/pce.12912},
  volume       = {40},
  year         = {2017},
}

Altmetric
View in Altmetric
Web of Science
Times cited: