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The mitochondrial DNA-associated protein SWIB5 influences mtDNA architecture and homologous recombination

Jonas Blomme (UGent), Olivier Van Aken (UGent), Jelle Van Leene (UGent), Teddy Jégu, Riet De Rycke (UGent), Michiel De Bruyne (UGent), Jasmien Vercruysse (UGent), Jonah Nolf (UGent), Twiggy Van Daele (UGent), Liesbeth De Milde (UGent), et al.
(2017) PLANT CELL. 29(5). p.1137-1156
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Biotechnology for a sustainable economy (Bio-Economy)
Abstract
In addition to the nucleus, mitochondria and chloroplasts in plant cells also contain genomes. Efficient DNA repair pathways are crucial in these organelles to fix damage resulting from endogenous and exogenous factors. Plant organellar genomes are complex compared with their animal counterparts, and although several plant-specific mediators of organelle DNA repair have been reported, many regulators remain to be identified. Here, we show that a mitochondrial SWI/SNF (nucleosome remodeling) complex B protein, SWIB5, is capable of associating with mitochondrial DNA (mtDNA) in Arabidopsis thaliana. Gain-and loss-of-function mutants provided evidence for a role of SWIB5 in influencing mtDNA architecture and homologous recombination at specific intermediate-sized repeats both under normal and genotoxic conditions. SWIB5 interacts with other mitochondrial SWIB proteins. Gene expression and mutant phenotypic analysis of SWIB5 and SWIB family members suggests a link between organellar genome maintenance and cell proliferation. Taken together, our work presents a protein family that influences mtDNA architecture and homologous recombination in plants and suggests a link between organelle functioning and plant development.
Keywords
DOUBLE-STRAND BREAKS, CHROMATIN REMODELING COMPLEXES, ARABIDOPSIS-THALIANA, BINDING-PROTEIN, H-NS, PLASMODIUM-FALCIPARUM, LEAF DEVELOPMENT, GENE-EXPRESSION, DAMAGE RESPONSE, SNF COMPLEX

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Citation

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Chicago
Blomme, Jonas, Olivier Van Aken, Jelle Van Leene, Teddy Jégu, Riet De Rycke, Michiel De Bruyne, Jasmien Vercruysse, et al. 2017. “The Mitochondrial DNA-associated Protein SWIB5 Influences mtDNA Architecture and Homologous Recombination.” Plant Cell 29 (5): 1137–1156.
APA
Blomme, Jonas, Van Aken, O., Van Leene, J., Jégu, T., De Rycke, R., De Bruyne, M., Vercruysse, J., et al. (2017). The mitochondrial DNA-associated protein SWIB5 influences mtDNA architecture and homologous recombination. PLANT CELL, 29(5), 1137–1156.
Vancouver
1.
Blomme J, Van Aken O, Van Leene J, Jégu T, De Rycke R, De Bruyne M, et al. The mitochondrial DNA-associated protein SWIB5 influences mtDNA architecture and homologous recombination. PLANT CELL. 2017;29(5):1137–56.
MLA
Blomme, Jonas, Olivier Van Aken, Jelle Van Leene, et al. “The Mitochondrial DNA-associated Protein SWIB5 Influences mtDNA Architecture and Homologous Recombination.” PLANT CELL 29.5 (2017): 1137–1156. Print.
@article{8526570,
  abstract     = {In addition to the nucleus, mitochondria and chloroplasts in plant cells also contain genomes. Efficient DNA repair pathways are crucial in these organelles to fix damage resulting from endogenous and exogenous factors. Plant organellar genomes are complex compared with their animal counterparts, and although several plant-specific mediators of organelle DNA repair have been reported, many regulators remain to be identified. Here, we show that a mitochondrial SWI/SNF (nucleosome remodeling) complex B protein, SWIB5, is capable of associating with mitochondrial DNA (mtDNA) in Arabidopsis thaliana. Gain-and loss-of-function mutants provided evidence for a role of SWIB5 in influencing mtDNA architecture and homologous recombination at specific intermediate-sized repeats both under normal and genotoxic conditions. SWIB5 interacts with other mitochondrial SWIB proteins. Gene expression and mutant phenotypic analysis of SWIB5 and SWIB family members suggests a link between organellar genome maintenance and cell proliferation. Taken together, our work presents a protein family that influences mtDNA architecture and homologous recombination in plants and suggests a link between organelle functioning and plant development.},
  author       = {Blomme, Jonas and Van Aken, Olivier and Van Leene, Jelle and J{\'e}gu, Teddy and De Rycke, Riet and De Bruyne, Michiel and Vercruysse, Jasmien and Nolf, Jonah and Van Daele, Twiggy and De Milde, Liesbeth and Vermeersch, Mattias and Colas des Francs-Small, Catherine and De Jaeger, Geert and Benhamed, Moussa and Millar, A Harvey and Inz{\'e}, Dirk and Gonzalez Sanchez, Nathalie},
  issn         = {1040-4651},
  journal      = {PLANT CELL},
  keyword      = {DOUBLE-STRAND BREAKS,CHROMATIN REMODELING COMPLEXES,ARABIDOPSIS-THALIANA,BINDING-PROTEIN,H-NS,PLASMODIUM-FALCIPARUM,LEAF DEVELOPMENT,GENE-EXPRESSION,DAMAGE RESPONSE,SNF COMPLEX},
  language     = {eng},
  number       = {5},
  pages        = {1137--1156},
  title        = {The mitochondrial DNA-associated protein SWIB5 influences mtDNA architecture and homologous recombination},
  url          = {http://dx.doi.org/10.1105/tpc.16.00899},
  volume       = {29},
  year         = {2017},
}

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