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Detection and toxicity evaluation of pyrrolizidine alkaloids in medicinal plants Gynura bicolor and Gynura divaricata collected from different Chinese locations

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Abstract
Two edible plants in Southeast Asia, Gynura bicolor and G.divaricata, are not only known to be nutritive but also useful as medicinal herbs. Previous phytochemical investigation of Gynura species showed the presence of hepatotoxic pyrrolizidine alkaloids (PAs), indicating the toxic risk of using these two plants. The present study was designed to analyze the distribution of PA components and tried to evaluate the preliminary toxicity of these two Gynura species. Eight samples of G.bicolor and G.divaricata from five different Chinese locations were collected and their specific PAs were qualitatively characterized by applying an UPLC/MS/MS spectrometry method. Using a pre-column derivatization HPLC method, the total retronecine ester-type PAs in their alkaloids extracts were quantitatively estimated as well. Finally, their genotoxicity was investigated with an effective high-throughput screening method referred to as Vitotox test and their potential cytotoxicity was tested on HepG2 cells. It was found that different types of PAs were widely present in Gynura species collected from south of China. Among them, no significant genotoxic effects were detected with serial concentrations through the present invitro assay. However, the cytotoxicity assay of Gynura plants collected from Jiangsu displayed weak activity at the concentration of 100mg/ml. It is important to note that this research validates in part the indication that the use of Gynura species requires caution.
Keywords
PERFORMANCE LIQUID-CHROMATOGRAPHY, TANDEM MASS-SPECTROMETRY, DNA-ADDUCTS, IN-VITRO, IDENTIFICATION, CONSTITUENTS, GENOTOXICITY, PROCUMBENS, EXTRACTS, RATS, Gynura bicolor, Gynura divaricata, Pyrrolizidine alkaloids, Genotoxic, activities, Cytotoxic activities

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Chicago
Chen, Jian, Han Lu, Lian-Xiang Fang, Wei-Lin Li, Luc Verschaeve, Zheng-Tao Wang, Norbert De Kimpe, and Sven Mangelinckx. 2017. “Detection and Toxicity Evaluation of Pyrrolizidine Alkaloids in Medicinal Plants Gynura Bicolor and Gynura Divaricata Collected from Different Chinese Locations.” Chemistry & Biodiversity 14 (2).
APA
Chen, Jian, Lu, H., Fang, L.-X., Li, W.-L., Verschaeve, L., Wang, Z.-T., De Kimpe, N., et al. (2017). Detection and toxicity evaluation of pyrrolizidine alkaloids in medicinal plants Gynura bicolor and Gynura divaricata collected from different Chinese locations. CHEMISTRY & BIODIVERSITY, 14(2).
Vancouver
1.
Chen J, Lu H, Fang L-X, Li W-L, Verschaeve L, Wang Z-T, et al. Detection and toxicity evaluation of pyrrolizidine alkaloids in medicinal plants Gynura bicolor and Gynura divaricata collected from different Chinese locations. CHEMISTRY & BIODIVERSITY. 2017;14(2).
MLA
Chen, Jian et al. “Detection and Toxicity Evaluation of Pyrrolizidine Alkaloids in Medicinal Plants Gynura Bicolor and Gynura Divaricata Collected from Different Chinese Locations.” CHEMISTRY & BIODIVERSITY 14.2 (2017): n. pag. Print.
@article{8526411,
  abstract     = {Two edible plants in Southeast Asia, Gynura bicolor and G.divaricata, are not only known to be nutritive but also useful as medicinal herbs. Previous phytochemical investigation of Gynura species showed the presence of hepatotoxic pyrrolizidine alkaloids (PAs), indicating the toxic risk of using these two plants. The present study was designed to analyze the distribution of PA components and tried to evaluate the preliminary toxicity of these two Gynura species. Eight samples of G.bicolor and G.divaricata from five different Chinese locations were collected and their specific PAs were qualitatively characterized by applying an UPLC/MS/MS spectrometry method. Using a pre-column derivatization HPLC method, the total retronecine ester-type PAs in their alkaloids extracts were quantitatively estimated as well. Finally, their genotoxicity was investigated with an effective high-throughput screening method referred to as Vitotox test and their potential cytotoxicity was tested on HepG2 cells. It was found that different types of PAs were widely present in Gynura species collected from south of China. Among them, no significant genotoxic effects were detected with serial concentrations through the present invitro assay. However, the cytotoxicity assay of Gynura plants collected from Jiangsu displayed weak activity at the concentration of 100mg/ml. It is important to note that this research validates in part the indication that the use of Gynura species requires caution.},
  articleno    = {e1600221},
  author       = {Chen, Jian and Lu, Han and Fang, Lian-Xiang and Li, Wei-Lin and Verschaeve, Luc and Wang, Zheng-Tao and De Kimpe, Norbert and Mangelinckx, Sven},
  issn         = {1612-1872},
  journal      = {CHEMISTRY & BIODIVERSITY},
  keywords     = {PERFORMANCE LIQUID-CHROMATOGRAPHY,TANDEM MASS-SPECTROMETRY,DNA-ADDUCTS,IN-VITRO,IDENTIFICATION,CONSTITUENTS,GENOTOXICITY,PROCUMBENS,EXTRACTS,RATS,Gynura bicolor,Gynura divaricata,Pyrrolizidine alkaloids,Genotoxic,activities,Cytotoxic activities},
  language     = {eng},
  number       = {2},
  pages        = {12},
  title        = {Detection and toxicity evaluation of pyrrolizidine alkaloids in medicinal plants Gynura bicolor and Gynura divaricata collected from different Chinese locations},
  url          = {http://dx.doi.org/10.1002/cbdv.201600221},
  volume       = {14},
  year         = {2017},
}

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