Advanced search
1 file | 392.86 KB Add to list

The preventive effects of two nutraceuticals on experimentally induced acute synovitis

(2017) EQUINE VETERINARY JOURNAL. 49(4). p.532-538
Author
Organization
Abstract
Background: Nutraceuticals are often used in the management of equine osteoarthritis, but scientific evidence of their efficacy is lacking. Objectives: To study the preventive effects of two new nutraceuticals after the experimental induction of synovitis in comparison with positive and negative control treatments. Study design: Blinded, controlled, randomised experiment. Methods: Twenty-four healthy Standardbred horses were randomly allocated to supplement AT (multi-ingredient, 28 days), supplement HP (collagen hydrolysate, 60 days), meloxicam (4 days) or placebo (60 days). Synovitis was induced in the right intercarpal joint by intra-articular injection of 0.5 ng lipopolysaccharide (LPS) of Escherichia coli while treatments were continued. Blood and synovial fluid were sampled before treatment, immediately prior to LPS injection, and at 8, 24 and 48 h post-injection. Synovial fluid samples were analysed for total nucleated cell count (TNCC), total protein (TP) and selected biomarkers (prostaglandin E2 [PGE2], interleukin-6 [IL-6], glycosaminoglycans [GAGs], type II collagen synthesis [CPII], matrix metalloproteinase [MMP]). Lameness was scored by visual examination and pressure plate analysis immediately prior to LPS injection, and at 8, 24 and 48 h post-injection. Clinical examinations were performed before treatment, immediately prior to LPS injection, at 2, 4 and 6 h post-injection, and then twice per day during the test period. Results: Before treatment and intra-articular challenge, there were no statistically significant differences among the treatment groups for any of the parameters. After intra-articular challenge, the placebo group showed significantly higher synovial fluid TP, TNCC and PGE2 compared with the meloxicam group, although the model did not induce a relevant amount of lameness. Both nutraceuticals resulted in significantly lower synovial fluid TP, TNCC and PGE2 compared with placebo. No statistical differences in IL-6, GAGs, CPII or MMPs were observed among treatment groups. No adverse effects were observed. Main limitations: Despite evidence of synovitis, lameness was too mild to detect. Conclusions: The preventive administration of these nutraceuticals showed anti-inflammatory effects in this validated synovitis model. Therefore, further studies of their clinical applicability are warranted.
Keywords
horse, LPS, arthritis, biomarkers, pressure plate, CHONDROITIN SULFATE, METHYLSULFONYLMETHANE MSM, GLUCOSAMINE HYDROCHLORIDE, COLLAGEN HYDROLYSATE, EQUINE CHONDROCYTES, JOINT DISEASE, DOUBLE-BLIND, OSTEOARTHRITIS, HORSES, INTERLEUKIN-1

Downloads

  • Van de Water et al-2016-Equine Veterinary Journal.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 392.86 KB

Citation

Please use this url to cite or link to this publication:

MLA
Van de Water, Eline et al. “The Preventive Effects of Two Nutraceuticals on Experimentally Induced Acute Synovitis.” EQUINE VETERINARY JOURNAL 49.4 (2017): 532–538. Print.
APA
Van de Water, E., Oosterlinck, M., Dumoulin, M., Korthagen, N., van Weeren, P., van den Broek, J., Everts, H., et al. (2017). The preventive effects of two nutraceuticals on experimentally induced acute synovitis. EQUINE VETERINARY JOURNAL, 49(4), 532–538.
Chicago author-date
Van de Water, Eline, Maarten Oosterlinck, Michèle Dumoulin, NM Korthagen, PR van Weeren, J van den Broek, H Everts, Frederik Pille, and DA van Doorn. 2017. “The Preventive Effects of Two Nutraceuticals on Experimentally Induced Acute Synovitis.” Equine Veterinary Journal 49 (4): 532–538.
Chicago author-date (all authors)
Van de Water, Eline, Maarten Oosterlinck, Michèle Dumoulin, NM Korthagen, PR van Weeren, J van den Broek, H Everts, Frederik Pille, and DA van Doorn. 2017. “The Preventive Effects of Two Nutraceuticals on Experimentally Induced Acute Synovitis.” Equine Veterinary Journal 49 (4): 532–538.
Vancouver
1.
Van de Water E, Oosterlinck M, Dumoulin M, Korthagen N, van Weeren P, van den Broek J, et al. The preventive effects of two nutraceuticals on experimentally induced acute synovitis. EQUINE VETERINARY JOURNAL. 2017;49(4):532–8.
IEEE
[1]
E. Van de Water et al., “The preventive effects of two nutraceuticals on experimentally induced acute synovitis,” EQUINE VETERINARY JOURNAL, vol. 49, no. 4, pp. 532–538, 2017.
@article{8526279,
  abstract     = {Background: Nutraceuticals are often used in the management of equine osteoarthritis, but scientific evidence of their efficacy is lacking.
Objectives: To study the preventive effects of two new nutraceuticals after the experimental induction of synovitis in comparison with positive and negative control treatments.
Study design: Blinded, controlled, randomised experiment.
Methods: Twenty-four healthy Standardbred horses were randomly allocated to supplement AT (multi-ingredient, 28 days), supplement HP (collagen hydrolysate, 60 days), meloxicam (4 days) or placebo (60 days). Synovitis was induced in the right intercarpal joint by intra-articular injection of 0.5 ng lipopolysaccharide (LPS) of Escherichia coli while treatments were continued. Blood and synovial fluid were sampled before treatment, immediately prior to LPS injection, and at 8, 24 and 48 h post-injection. Synovial fluid samples were analysed for total nucleated cell count (TNCC), total protein (TP) and selected biomarkers (prostaglandin E2 [PGE2], interleukin-6 [IL-6], glycosaminoglycans [GAGs], type II collagen synthesis [CPII], matrix metalloproteinase [MMP]). Lameness was scored by visual examination and pressure plate analysis immediately prior to LPS injection, and at 8, 24 and 48 h post-injection. Clinical examinations were performed before treatment, immediately prior to LPS injection, at 2, 4 and 6 h post-injection, and then twice per day during the test period.
Results: Before treatment and intra-articular challenge, there were no statistically significant differences among the treatment groups for any of the parameters. After intra-articular challenge, the placebo group showed significantly higher synovial fluid TP, TNCC and PGE2 compared with the meloxicam group, although the model did not induce a relevant amount of lameness. Both nutraceuticals resulted in significantly lower synovial fluid TP, TNCC and PGE2 compared with placebo. No statistical differences in IL-6, GAGs, CPII or MMPs were observed among treatment groups. No adverse effects were observed.
Main limitations: Despite evidence of synovitis, lameness was too mild to detect.
Conclusions: The preventive administration of these nutraceuticals showed anti-inflammatory effects in this validated synovitis model. Therefore, further studies of their clinical applicability are warranted.},
  author       = {Van de Water, Eline and Oosterlinck, Maarten and Dumoulin, Michèle and Korthagen, NM and van Weeren, PR and van den Broek, J and Everts, H and Pille, Frederik and van Doorn, DA},
  issn         = {0425-1644},
  journal      = {EQUINE VETERINARY JOURNAL},
  keywords     = {horse,LPS,arthritis,biomarkers,pressure plate,CHONDROITIN SULFATE,METHYLSULFONYLMETHANE MSM,GLUCOSAMINE HYDROCHLORIDE,COLLAGEN HYDROLYSATE,EQUINE CHONDROCYTES,JOINT DISEASE,DOUBLE-BLIND,OSTEOARTHRITIS,HORSES,INTERLEUKIN-1},
  language     = {eng},
  number       = {4},
  pages        = {532--538},
  title        = {The preventive effects of two nutraceuticals on experimentally induced acute synovitis},
  url          = {http://dx.doi.org/10.1111/evj.12629},
  volume       = {49},
  year         = {2017},
}

Altmetric
View in Altmetric
Web of Science
Times cited: