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Cellulose synthesis by Komagataeibacter rhaeticus strain P 1463 isolated from Kombucha

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Abstract
Isolate B17 from Kombucha was estimated to be an efficient producer of bacterial cellulose (BC). The isolate was deposited under the number P 1463 and identified as Komagataeibacter rhaeticus by comparing a generated amplified fragment length polymorphism (AFLP (TM)) DNA fingerprint against a reference database. Static cultivation of the K. rhaeticus strain P 1463 in Hestrin and Schramm (HS) medium resulted in 4.40 +/- 0.22 g/L BC being produced, corresponding to a BC yield from glucose of 25.30 +/- 1.78 %, when the inoculum was made with a modified HS medium containing 10 g/L glucose. Fermentations for 5 days using media containing apple juice with analogous carbon source concentrations resulted in 4.77 +/- 0.24 g/L BC being synthesised, corresponding to a yield from the consumed sugars (glucose, fructose and sucrose) of 37.00 +/- 2.61 %. The capacity of K. rhaeticus strain P 1463 to synthesise BC was found to be much higher than that of two reference strains for cellulose production, Komagataeibacter xylinus DSM 46604 and Komagataeibacter hansenii DSM 5602(T), and was also considerably higher than that of K. hansenii strain B22, isolated from another Kombucha sample. The BC synthesised by K. rhaeticus strain P 1463 after 40 days of cultivation in HS medium with additional glucose supplemented to the cell culture during cultivation was shown to have a degree of polymerization of 3300.0 +/- 122.1 glucose units, a tensile strength of 65.50 +/- 3.27 MPa and a length at break of 16.50 +/- 0.83 km. For the other strains, these properties did not exceed 25.60 +/- 1.28 MPa and 15.20 +/- 0.76 km.
Keywords
Komagataeibacterrhaeticus, Bacterialcellulose, Productivity, Properties, ACETIC-ACID BACTERIA, GLUCONACETOBACTER-HANSENII, ACETOBACTERACEAE, XYLINUS, FAMILY, NOV.

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Citation

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Chicago
Semjonovs, Pavels, Maija Ruklisha, Longina Paegle, Madara Saka, Rita Treimane, Marite Skute, Linda Rozenberga, Laura Vikele, Martins Sabovics, and Ilse Cleenwerck. 2017. “Cellulose Synthesis by Komagataeibacter Rhaeticus Strain P 1463 Isolated from Kombucha.” Applied Microbiology and Biotechnology 101 (3): 1003–1012.
APA
Semjonovs, P., Ruklisha, M., Paegle, L., Saka, M., Treimane, R., Skute, M., Rozenberga, L., et al. (2017). Cellulose synthesis by Komagataeibacter rhaeticus strain P 1463 isolated from Kombucha. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 101(3), 1003–1012.
Vancouver
1.
Semjonovs P, Ruklisha M, Paegle L, Saka M, Treimane R, Skute M, et al. Cellulose synthesis by Komagataeibacter rhaeticus strain P 1463 isolated from Kombucha. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2017;101(3):1003–12.
MLA
Semjonovs, Pavels, Maija Ruklisha, Longina Paegle, et al. “Cellulose Synthesis by Komagataeibacter Rhaeticus Strain P 1463 Isolated from Kombucha.” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 101.3 (2017): 1003–1012. Print.
@article{8526068,
  abstract     = {Isolate B17 from Kombucha was estimated to be an efficient producer of bacterial cellulose (BC). The isolate was deposited under the number P 1463 and identified as Komagataeibacter rhaeticus by comparing a generated amplified fragment length polymorphism (AFLP (TM)) DNA fingerprint against a reference database. Static cultivation of the K. rhaeticus strain P 1463 in Hestrin and Schramm (HS) medium resulted in 4.40 +/- 0.22 g/L BC being produced, corresponding to a BC yield from glucose of 25.30 +/- 1.78 %, when the inoculum was made with a modified HS medium containing 10 g/L glucose. Fermentations for 5 days using media containing apple juice with analogous carbon source concentrations resulted in 4.77 +/- 0.24 g/L BC being synthesised, corresponding to a yield from the consumed sugars (glucose, fructose and sucrose) of 37.00 +/- 2.61 %. The capacity of K. rhaeticus strain P 1463 to synthesise BC was found to be much higher than that of two reference strains for cellulose production, Komagataeibacter xylinus DSM 46604 and Komagataeibacter hansenii DSM 5602(T), and was also considerably higher than that of K. hansenii strain B22, isolated from another Kombucha sample. The BC synthesised by K. rhaeticus strain P 1463 after 40 days of cultivation in HS medium with additional glucose supplemented to the cell culture during cultivation was shown to have a degree of polymerization of 3300.0 +/- 122.1 glucose units, a tensile strength of 65.50 +/- 3.27 MPa and a length at break of 16.50 +/- 0.83 km. For the other strains, these properties did not exceed 25.60 +/- 1.28 MPa and 15.20 +/- 0.76 km.},
  author       = {Semjonovs, Pavels and Ruklisha, Maija and Paegle, Longina and Saka, Madara and Treimane, Rita and Skute, Marite and Rozenberga, Linda and Vikele, Laura and Sabovics, Martins and Cleenwerck, Ilse},
  issn         = {0175-7598},
  journal      = {APPLIED MICROBIOLOGY AND BIOTECHNOLOGY},
  keywords     = {Komagataeibacterrhaeticus,Bacterialcellulose,Productivity,Properties,ACETIC-ACID BACTERIA,GLUCONACETOBACTER-HANSENII,ACETOBACTERACEAE,XYLINUS,FAMILY,NOV.},
  language     = {eng},
  number       = {3},
  pages        = {1003--1012},
  title        = {Cellulose synthesis by Komagataeibacter rhaeticus strain P 1463 isolated from Kombucha},
  url          = {http://dx.doi.org/10.1007/s00253-016-7761-8},
  volume       = {101},
  year         = {2017},
}

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