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Multiple Locus Variable number of tandem repeat Analysis : a molecular genotyping tool for Paenibacillus larvae

Tine Descamps (UGent) , Lina De Smet (UGent) , Pieter Stragier (UGent) , Paul De Vos (UGent) and Dirk de Graaf (UGent)
(2016) MICROBIAL BIOTECHNOLOGY. 9(6). p.772-781
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Abstract
American Foulbrood, caused by Paenibacillus larvae, is the most severe bacterial disease of honey bees (Apis mellifera). To perform genotyping of P.larvae in an epidemiological context, there is a need of a fast and cheap method with a high resolution. Here, we propose Multiple Locus Variable number of tandem repeat Analysis (MLVA). MLVA has been used for typing a collection of 209 P.larvae strains from which 23 different MLVA types could be identified. Moreover, the developed methodology not only permits the identification of the four Enterobacterial Repetitive Intergenic Consensus (ERIC) genotypes, but allows also a discriminatory subdivision of the most dominant ERIC type I and ERIC type II genotypes. A biogeographical study has been conducted showing a significant correlation between MLVA genotype and the geographical region where it was isolated.
Keywords
AMERICAN FOULBROOD DISEASE, FIELD GEL-ELECTROPHORESIS, 16S, RIBOSOMAL-RNA, SUBSP LARVAE, HONEY-BEES, GENETIC DIVERSITY, BACILLUS, PULVIFACIENS, BACTERIAL PATHOGEN, ESCHERICHIA-COLI, APIS-MELLIFERA

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MLA
Descamps, Tine, et al. “Multiple Locus Variable Number of Tandem Repeat Analysis : A Molecular Genotyping Tool for Paenibacillus Larvae.” MICROBIAL BIOTECHNOLOGY, vol. 9, no. 6, 2016, pp. 772–81, doi:10.1111/1751-7915.12375.
APA
Descamps, T., De Smet, L., Stragier, P., De Vos, P., & de Graaf, D. (2016). Multiple Locus Variable number of tandem repeat Analysis : a molecular genotyping tool for Paenibacillus larvae. MICROBIAL BIOTECHNOLOGY, 9(6), 772–781. https://doi.org/10.1111/1751-7915.12375
Chicago author-date
Descamps, Tine, Lina De Smet, Pieter Stragier, Paul De Vos, and Dirk de Graaf. 2016. “Multiple Locus Variable Number of Tandem Repeat Analysis : A Molecular Genotyping Tool for Paenibacillus Larvae.” MICROBIAL BIOTECHNOLOGY 9 (6): 772–81. https://doi.org/10.1111/1751-7915.12375.
Chicago author-date (all authors)
Descamps, Tine, Lina De Smet, Pieter Stragier, Paul De Vos, and Dirk de Graaf. 2016. “Multiple Locus Variable Number of Tandem Repeat Analysis : A Molecular Genotyping Tool for Paenibacillus Larvae.” MICROBIAL BIOTECHNOLOGY 9 (6): 772–781. doi:10.1111/1751-7915.12375.
Vancouver
1.
Descamps T, De Smet L, Stragier P, De Vos P, de Graaf D. Multiple Locus Variable number of tandem repeat Analysis : a molecular genotyping tool for Paenibacillus larvae. MICROBIAL BIOTECHNOLOGY. 2016;9(6):772–81.
IEEE
[1]
T. Descamps, L. De Smet, P. Stragier, P. De Vos, and D. de Graaf, “Multiple Locus Variable number of tandem repeat Analysis : a molecular genotyping tool for Paenibacillus larvae,” MICROBIAL BIOTECHNOLOGY, vol. 9, no. 6, pp. 772–781, 2016.
@article{8526031,
  abstract     = {{American Foulbrood, caused by Paenibacillus larvae, is the most severe bacterial disease of honey bees (Apis mellifera). To perform genotyping of P.larvae in an epidemiological context, there is a need of a fast and cheap method with a high resolution. Here, we propose Multiple Locus Variable number of tandem repeat Analysis (MLVA). MLVA has been used for typing a collection of 209 P.larvae strains from which 23 different MLVA types could be identified. Moreover, the developed methodology not only permits the identification of the four Enterobacterial Repetitive Intergenic Consensus (ERIC) genotypes, but allows also a discriminatory subdivision of the most dominant ERIC type I and ERIC type II genotypes. A biogeographical study has been conducted showing a significant correlation between MLVA genotype and the geographical region where it was isolated.}},
  author       = {{Descamps, Tine and De Smet, Lina and Stragier, Pieter and De Vos, Paul and de Graaf, Dirk}},
  issn         = {{1751-7915}},
  journal      = {{MICROBIAL BIOTECHNOLOGY}},
  keywords     = {{AMERICAN FOULBROOD DISEASE,FIELD GEL-ELECTROPHORESIS,16S,RIBOSOMAL-RNA,SUBSP LARVAE,HONEY-BEES,GENETIC DIVERSITY,BACILLUS,PULVIFACIENS,BACTERIAL PATHOGEN,ESCHERICHIA-COLI,APIS-MELLIFERA}},
  language     = {{eng}},
  number       = {{6}},
  pages        = {{772--781}},
  title        = {{Multiple Locus Variable number of tandem repeat Analysis : a molecular genotyping tool for Paenibacillus larvae}},
  url          = {{http://doi.org/10.1111/1751-7915.12375}},
  volume       = {{9}},
  year         = {{2016}},
}

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