Advanced search
1 file | 3.70 MB

Epitope mapping and kinetics of CD4 T cell immunity to pneumonia virus of mice in the C57BL/6 strain

Author
Organization
Abstract
Pneumonia virus of mice (PVM) infection has been widely used as a rodent model to study the closely related human respiratory syncytial virus (hRSV). While T cells are indispensable for viral clearance, they also contribute to immunopathology. To gain more insight into mechanistic details, novel tools are needed that allow to study virus-specific T cells in C57BL/6 mice as the majority of transgenic mice are only available on this background. While PVM-specific CD8 T cell epitopes were recently described, so far no PVM-specific CD4 T cell epitopes have been identified within the C57BL/6 strain. Therefore, we set out to map H2-IAb-restricted epitopes along the PVM proteome. By means of in silico prediction and subsequent functional validation, we were able to identify a MHCII-restricted CD4 T cell epitope, corresponding to amino acids 37-47 in the PVM matrix protein (M37-47). Using this newly identified MHCII-restricted M37-47 epitope and a previously described MHCI-restricted N339-347 epitope, we generated peptide-loaded MHCII and MHCI tetramers and characterized the dynamics of virus-specific CD4 and CD8 T cell responses in vivo. The findings of this study can provide a basis for detailed investigation of T cell-mediated immune responses to PVM in a variety of genetically modified C57BL/6 mice.
Keywords
RESPIRATORY SYNCYTIAL VIRUS, PNEUMOVIRUS INFECTION, ATTACHMENT, GLYCOPROTEIN, DENDRITIC CELLS, RESPONSES, PATHOGENESIS, DISEASE, PEPTIDE, MEMORY, SEQUENCE

Downloads

  • 2860 17Vandersarren.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 3.70 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Vandersarren, Lana, Cedric Bosteels, Manon Vanheerswynghels, James J Moon, Andrew J Easton, Gert Van Isterdael, Sophie Janssens, Bart Lambrecht, and Mary van Helden. 2017. “Epitope Mapping and Kinetics of CD4 T Cell Immunity to Pneumonia Virus of Mice in the C57BL/6 Strain.” Scientific Reports 7.
APA
Vandersarren, L., Bosteels, C., Vanheerswynghels, M., Moon, J. J., Easton, A. J., Van Isterdael, G., Janssens, S., et al. (2017). Epitope mapping and kinetics of CD4 T cell immunity to pneumonia virus of mice in the C57BL/6 strain. SCIENTIFIC REPORTS, 7.
Vancouver
1.
Vandersarren L, Bosteels C, Vanheerswynghels M, Moon JJ, Easton AJ, Van Isterdael G, et al. Epitope mapping and kinetics of CD4 T cell immunity to pneumonia virus of mice in the C57BL/6 strain. SCIENTIFIC REPORTS. 2017;7.
MLA
Vandersarren, Lana, Cedric Bosteels, Manon Vanheerswynghels, et al. “Epitope Mapping and Kinetics of CD4 T Cell Immunity to Pneumonia Virus of Mice in the C57BL/6 Strain.” SCIENTIFIC REPORTS 7 (2017): n. pag. Print.
@article{8525798,
  abstract     = {Pneumonia virus of mice (PVM) infection has been widely used as a rodent model to study the closely related human respiratory syncytial virus (hRSV). While T cells are indispensable for viral clearance, they also contribute to immunopathology. To gain more insight into mechanistic details, novel tools are needed that allow to study virus-specific T cells in C57BL/6 mice as the majority of transgenic mice are only available on this background. While PVM-specific CD8 T cell epitopes were recently described, so far no PVM-specific CD4 T cell epitopes have been identified within the C57BL/6 strain. Therefore, we set out to map H2-IAb-restricted epitopes along the PVM proteome. By means of in silico prediction and subsequent functional validation, we were able to identify a MHCII-restricted CD4 T cell epitope, corresponding to amino acids 37-47 in the PVM matrix protein (M37-47). Using this newly identified MHCII-restricted M37-47 epitope and a previously described MHCI-restricted N339-347 epitope, we generated peptide-loaded MHCII and MHCI tetramers and characterized the dynamics of virus-specific CD4 and CD8 T cell responses in vivo. The findings of this study can provide a basis for detailed investigation of T cell-mediated immune responses to PVM in a variety of genetically modified C57BL/6 mice.},
  articleno    = {3472},
  author       = {Vandersarren, Lana and Bosteels, Cedric and Vanheerswynghels, Manon and Moon, James J and Easton, Andrew J and Van Isterdael, Gert and Janssens, Sophie and Lambrecht, Bart and van Helden, Mary},
  issn         = {2045-2322},
  journal      = {SCIENTIFIC REPORTS},
  keyword      = {RESPIRATORY SYNCYTIAL VIRUS,PNEUMOVIRUS INFECTION,ATTACHMENT,GLYCOPROTEIN,DENDRITIC CELLS,RESPONSES,PATHOGENESIS,DISEASE,PEPTIDE,MEMORY,SEQUENCE},
  language     = {eng},
  pages        = {10},
  title        = {Epitope mapping and kinetics of CD4 T cell immunity to pneumonia virus of mice in the C57BL/6 strain},
  url          = {http://dx.doi.org/10.1038/s41598-017-03042-y},
  volume       = {7},
  year         = {2017},
}

Altmetric
View in Altmetric
Web of Science
Times cited: