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Epitope mapping and kinetics of CD4 T cell immunity to pneumonia virus of mice in the C57BL/6 strain

Lana Vandersarren UGent, Cedric Bosteels UGent, Manon Vanheerswynghels UGent, James J Moon, Andrew J Easton, Gert Van Isterdael UGent, Sophie Janssens UGent, Bart Lambrecht UGent and Mary van Helden (2017) SCIENTIFIC REPORTS. 7.
abstract
Pneumonia virus of mice (PVM) infection has been widely used as a rodent model to study the closely related human respiratory syncytial virus (hRSV). While T cells are indispensable for viral clearance, they also contribute to immunopathology. To gain more insight into mechanistic details, novel tools are needed that allow to study virus-specific T cells in C57BL/6 mice as the majority of transgenic mice are only available on this background. While PVM-specific CD8 T cell epitopes were recently described, so far no PVM-specific CD4 T cell epitopes have been identified within the C57BL/6 strain. Therefore, we set out to map H2-IAb-restricted epitopes along the PVM proteome. By means of in silico prediction and subsequent functional validation, we were able to identify a MHCII-restricted CD4 T cell epitope, corresponding to amino acids 37-47 in the PVM matrix protein (M37-47). Using this newly identified MHCII-restricted M37-47 epitope and a previously described MHCI-restricted N339-347 epitope, we generated peptide-loaded MHCII and MHCI tetramers and characterized the dynamics of virus-specific CD4 and CD8 T cell responses in vivo. The findings of this study can provide a basis for detailed investigation of T cell-mediated immune responses to PVM in a variety of genetically modified C57BL/6 mice.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
RESPIRATORY SYNCYTIAL VIRUS, PNEUMOVIRUS INFECTION, ATTACHMENT, GLYCOPROTEIN, DENDRITIC CELLS, RESPONSES, PATHOGENESIS, DISEASE, PEPTIDE, MEMORY, SEQUENCE
journal title
SCIENTIFIC REPORTS
Sci. Rep.
volume
7
article number
3472
pages
10 pages
Web of Science type
Article
Web of Science id
000403318400026
ISSN
2045-2322
DOI
10.1038/s41598-017-03042-y
language
English
UGent publication?
yes
classification
A1
copyright statement
Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
id
8525798
handle
http://hdl.handle.net/1854/LU-8525798
date created
2017-06-29 15:04:11
date last changed
2017-07-04 08:55:13
@article{8525798,
  abstract     = {Pneumonia virus of mice (PVM) infection has been widely used as a rodent model to study the closely related human respiratory syncytial virus (hRSV). While T cells are indispensable for viral clearance, they also contribute to immunopathology. To gain more insight into mechanistic details, novel tools are needed that allow to study virus-specific T cells in C57BL/6 mice as the majority of transgenic mice are only available on this background. While PVM-specific CD8 T cell epitopes were recently described, so far no PVM-specific CD4 T cell epitopes have been identified within the C57BL/6 strain. Therefore, we set out to map H2-IAb-restricted epitopes along the PVM proteome. By means of in silico prediction and subsequent functional validation, we were able to identify a MHCII-restricted CD4 T cell epitope, corresponding to amino acids 37-47 in the PVM matrix protein (M37-47). Using this newly identified MHCII-restricted M37-47 epitope and a previously described MHCI-restricted N339-347 epitope, we generated peptide-loaded MHCII and MHCI tetramers and characterized the dynamics of virus-specific CD4 and CD8 T cell responses in vivo. The findings of this study can provide a basis for detailed investigation of T cell-mediated immune responses to PVM in a variety of genetically modified C57BL/6 mice.},
  articleno    = {3472},
  author       = {Vandersarren, Lana and Bosteels, Cedric and Vanheerswynghels, Manon and Moon, James J and Easton, Andrew J and Van Isterdael, Gert and Janssens, Sophie and Lambrecht, Bart and van Helden, Mary},
  issn         = {2045-2322},
  journal      = {SCIENTIFIC REPORTS},
  keyword      = {RESPIRATORY SYNCYTIAL VIRUS,PNEUMOVIRUS INFECTION,ATTACHMENT,GLYCOPROTEIN,DENDRITIC CELLS,RESPONSES,PATHOGENESIS,DISEASE,PEPTIDE,MEMORY,SEQUENCE},
  language     = {eng},
  pages        = {10},
  title        = {Epitope mapping and kinetics of CD4 T cell immunity to pneumonia virus of mice in the C57BL/6 strain},
  url          = {http://dx.doi.org/10.1038/s41598-017-03042-y},
  volume       = {7},
  year         = {2017},
}

Chicago
Vandersarren, Lana, Cedric Bosteels, Manon Vanheerswynghels, James J Moon, Andrew J Easton, Gert Van Isterdael, Sophie Janssens, Bart Lambrecht, and Mary van Helden. 2017. “Epitope Mapping and Kinetics of CD4 T Cell Immunity to Pneumonia Virus of Mice in the C57BL/6 Strain.” Scientific Reports 7.
APA
Vandersarren, L., Bosteels, C., Vanheerswynghels, M., Moon, J. J., Easton, A. J., Van Isterdael, G., Janssens, S., et al. (2017). Epitope mapping and kinetics of CD4 T cell immunity to pneumonia virus of mice in the C57BL/6 strain. SCIENTIFIC REPORTS, 7.
Vancouver
1.
Vandersarren L, Bosteels C, Vanheerswynghels M, Moon JJ, Easton AJ, Van Isterdael G, et al. Epitope mapping and kinetics of CD4 T cell immunity to pneumonia virus of mice in the C57BL/6 strain. SCIENTIFIC REPORTS. 2017;7.
MLA
Vandersarren, Lana, Cedric Bosteels, Manon Vanheerswynghels, et al. “Epitope Mapping and Kinetics of CD4 T Cell Immunity to Pneumonia Virus of Mice in the C57BL/6 Strain.” SCIENTIFIC REPORTS 7 (2017): n. pag. Print.