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Laser ablation-inductively coupled plasma-mass spectrometry for quantitative mapping of the copper distribution in liver tissue sections from mice with liver disease induced by common bile duct ligation

Marta Costas Rodriguez UGent, Thibaut Van Acker UGent, Agustina Ari Murti Budi Hastuti UGent, Lindsey Devisscher UGent, Sanne Van Campenhout UGent, Hans Van Vlierberghe UGent and Frank Vanhaecke UGent (2017) JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY. 32(9). p.1805-1812
abstract
Laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) was deployed for quantitative mapping of the Cu distribution in cryo-sections of liver tissue from mice with cholestatic liver disease induced via common bile duct ligation (CBDL). Cu distribution maps of the liver sections were obtained from the CBDL-operated mice sacrificed at different time points (2, 4 and 6 weeks) after the surgical intervention and compared with those of the corresponding control (sham-operated) mice. Cu quantification was accomplished versus matrix-matched thin sections of spiked liver tissue homogenates and versus spiked gelatin droplet standards. No statistical differences were obtained between the results using the two calibration approaches and thus, both were considered suitable for quantitative Cu bioimaging of liver cryo-sections. On the basis of practical considerations, i.e. simplicity, low cost and availability of the material, spiked gelatin droplet standards are the preferred choice for quantitative determination of the Cu distribution in liver tissue cryo-sections. An inhomogeneous hepatic Cu distribution was observed in the CBDL mice, in contrast to the homogeneous hepatic Cu distribution established for the sham-operated mice. The Cu levels increased with the progression of the disease and a strong accumulation was observed in some necrotic areas. High-resolution LA-ICP-MS bioimaging, using a circular spot size of 2 mm, was suitable for the visualization of the Cu distribution in liver tissue on a (sub-) cellular level. In addition to the quantitative Cu mapping, the spatial distribution of Zn was also monitored in the liver cryo-sections of the control and the 2, 4 and 6 week CBDL mice, but in all cases, Zn was homogeneously distributed across the tissue.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
LA-ICP-MS, MOLECULAR-MECHANISMS, BIOLOGICAL SAMPLES, WILSONS-DISEASE, FIBROSIS, ELEMENTS, ACCUMULATION, CALIBRATION, STRATEGIES, MODEL
journal title
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
J. Anal. At. Spectrom.
volume
32
issue
9
pages
1805 - 1812
Web of Science type
Article
Web of Science id
000409006400020
ISSN
0267-9477
1364-5544
DOI
10.1039/c7ja00134g
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8525406
handle
http://hdl.handle.net/1854/LU-8525406
date created
2017-06-27 15:16:43
date last changed
2017-09-18 14:46:31
@article{8525406,
  abstract     = {Laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) was deployed for quantitative mapping of the Cu distribution in cryo-sections of liver tissue from mice with cholestatic liver disease induced via common bile duct ligation (CBDL). Cu distribution maps of the liver sections were obtained from the CBDL-operated mice sacrificed at different time points (2, 4 and 6 weeks) after the surgical intervention and compared with those of the corresponding control (sham-operated) mice. Cu quantification was accomplished versus matrix-matched thin sections of spiked liver tissue homogenates and versus spiked gelatin droplet standards. No statistical differences were obtained between the results using the two calibration approaches and thus, both were considered suitable for quantitative Cu bioimaging of liver cryo-sections. On the basis of practical considerations, i.e. simplicity, low cost and availability of the material, spiked gelatin droplet standards are the preferred choice for quantitative determination of the Cu distribution in liver tissue cryo-sections. An inhomogeneous hepatic Cu distribution was observed in the CBDL mice, in contrast to the homogeneous hepatic Cu distribution established for the sham-operated mice. The Cu levels increased with the progression of the disease and a strong accumulation was observed in some necrotic areas. High-resolution LA-ICP-MS bioimaging, using a circular spot size of 2 mm, was suitable for the visualization of the Cu distribution in liver tissue on a (sub-) cellular level. In addition to the quantitative Cu mapping, the spatial distribution of Zn was also monitored in the liver cryo-sections of the control and the 2, 4 and 6 week CBDL mice, but in all cases, Zn was homogeneously distributed across the tissue.},
  author       = {Costas Rodriguez, Marta and Van Acker, Thibaut and Hastuti, Agustina Ari Murti Budi and Devisscher, Lindsey and Van Campenhout, Sanne and Van Vlierberghe, Hans and Vanhaecke, Frank},
  issn         = {0267-9477},
  journal      = {JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY},
  keyword      = {LA-ICP-MS,MOLECULAR-MECHANISMS,BIOLOGICAL SAMPLES,WILSONS-DISEASE,FIBROSIS,ELEMENTS,ACCUMULATION,CALIBRATION,STRATEGIES,MODEL},
  language     = {eng},
  number       = {9},
  pages        = {1805--1812},
  title        = {Laser ablation-inductively coupled plasma-mass spectrometry for quantitative mapping of the copper distribution in liver tissue sections from mice with liver disease induced by common bile duct ligation},
  url          = {http://dx.doi.org/10.1039/c7ja00134g},
  volume       = {32},
  year         = {2017},
}

Chicago
Costas Rodriguez, Marta, Thibaut Van Acker, Agustina Ari Murti Budi Hastuti, Lindsey Devisscher, Sanne Van Campenhout, Hans Van Vlierberghe, and Frank Vanhaecke. 2017. “Laser Ablation-inductively Coupled Plasma-mass Spectrometry for Quantitative Mapping of the Copper Distribution in Liver Tissue Sections from Mice with Liver Disease Induced by Common Bile Duct Ligation.” Journal of Analytical Atomic Spectrometry 32 (9): 1805–1812.
APA
Costas Rodriguez, M., Van Acker, T., Hastuti, A. A. M. B., Devisscher, L., Van Campenhout, S., Van Vlierberghe, H., & Vanhaecke, F. (2017). Laser ablation-inductively coupled plasma-mass spectrometry for quantitative mapping of the copper distribution in liver tissue sections from mice with liver disease induced by common bile duct ligation. JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY, 32(9), 1805–1812.
Vancouver
1.
Costas Rodriguez M, Van Acker T, Hastuti AAMB, Devisscher L, Van Campenhout S, Van Vlierberghe H, et al. Laser ablation-inductively coupled plasma-mass spectrometry for quantitative mapping of the copper distribution in liver tissue sections from mice with liver disease induced by common bile duct ligation. JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY. 2017;32(9):1805–12.
MLA
Costas Rodriguez, Marta, Thibaut Van Acker, Agustina Ari Murti Budi Hastuti, et al. “Laser Ablation-inductively Coupled Plasma-mass Spectrometry for Quantitative Mapping of the Copper Distribution in Liver Tissue Sections from Mice with Liver Disease Induced by Common Bile Duct Ligation.” JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY 32.9 (2017): 1805–1812. Print.