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Intratumoural interleukin 12 gene therapy stimulates the immune system and decreases angiogenesis in dogs with spontaneous cancer

Laetitia Cicchelero E, Sofie Denies, Hendrik Haers, Katrien Vanderperren UGent, Emmelie Stock UGent, Leen Van Brantegem UGent, Hilde De Rooster UGent and Niek Sanders UGent (2017) VETERINARY AND COMPARATIVE ONCOLOGY. 15(4). p.1187-1205
abstract
Interleukin 12 (IL-12) is a powerful immunostimulatory cytokine with a strong antitumoural activity. In this work, the immunological, anti-angiogenic and clinical effects of three consecutive intratumoural IL-12 electrogene therapy (EGT) treatments were evaluated in nine dogs with spontaneous cancer. In all the dogs, tumour biopsies and blood samples were taken prior, during and after the intratumoural IL-12 EGT (on days 1, 8, 35 and 1, 3, 8, 15, 35, respectively). An initial decrease in immune cells was followed by an increase above baseline 1-3 weeks after treatment initiation. Interestingly, the decrease in peripheral leukocytes 2 days after the first intratumoural IL-12 EGT coincided with erythema and tumour swelling. Transient increases of IL-12 and interferon were measured in the serum and the tumour tissue, whereas IL-10 transiently increased only in the serum. The effect of intratumoural IL-12 EGT on the levels of IL-24 and vascular endothelial growth factor in the sera and tumour biopsies differed per dog. Via contrast-enhanced ultrasound (US) (on days 1, 8 and 35), we demonstrated that intratumoural IL-12 EGT resulted in a significant decrease of the relative blood volume and blood flow speed in the tumour compared with baseline. Metastases were present in two dogs. In one of these dogs, IL-12 EGT of the primary tumour caused a transient partial regression of the metastases, but not of the primary tumour. The second dog with metastases did not survive long enough to complete the entire treatment cycle. Despite encouraging immunostimulatory and anti-angiogenic effects after intratumoural IL-12 EGT, no clinically relevant outcomes were observed in this study, as persistent tumour regression could not be obtained. On the other hand, the laboratory and US results hold great promise for combinatorial strategies of intratumoural IL-12 EGT with conventional antitumour (immuno)therapies.
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author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
angiogenesis, contrast-enhanced ultrasound, electroporation, gene transfer, immune stimulation, interleukin 12, spontaneous tumours, CONTRAST-ENHANCED ULTRASOUND, NATURAL-KILLER-CELLS, HUMAN T-CELLS, TUMOR-GROWTH, METASTATIC MELANOMA, GAMMA-INTERFERON, IL-12 RECEPTOR, LYMPH-NODE, IFN-GAMMA, NK-CELLS
journal title
VETERINARY AND COMPARATIVE ONCOLOGY
Vet. Comp. Oncol.
volume
15
issue
4
pages
1187 - 1205
Web of Science type
Article
Web of Science id
000414681400008
ISSN
1476-5810
DOI
10.1111/vco.12255
language
English
UGent publication?
yes
classification
A1
additional info
the last two authors are co-last authors
copyright statement
I have transferred the copyright for this publication to the publisher
id
8525386
handle
http://hdl.handle.net/1854/LU-8525386
date created
2017-06-27 13:33:16
date last changed
2018-02-07 13:08:04
@article{8525386,
  abstract     = {Interleukin 12 (IL-12) is a powerful immunostimulatory cytokine with a strong antitumoural activity. In this work, the immunological, anti-angiogenic and clinical effects of three consecutive intratumoural IL-12 electrogene therapy (EGT) treatments were evaluated in nine dogs with spontaneous cancer. In all the dogs, tumour biopsies and blood samples were taken prior, during and after the intratumoural IL-12 EGT (on days 1, 8, 35 and 1, 3, 8, 15, 35, respectively). An initial decrease in immune cells was followed by an increase above baseline 1-3 weeks after treatment initiation. Interestingly, the decrease in peripheral leukocytes 2 days after the first intratumoural IL-12 EGT coincided with erythema and tumour swelling. Transient increases of IL-12 and interferon were measured in the serum and the tumour tissue, whereas IL-10 transiently increased only in the serum. The effect of intratumoural IL-12 EGT on the levels of IL-24 and vascular endothelial growth factor in the sera and tumour biopsies differed per dog. Via contrast-enhanced ultrasound (US) (on days 1, 8 and 35), we demonstrated that intratumoural IL-12 EGT resulted in a significant decrease of the relative blood volume and blood flow speed in the tumour compared with baseline. Metastases were present in two dogs. In one of these dogs, IL-12 EGT of the primary tumour caused a transient partial regression of the metastases, but not of the primary tumour. The second dog with metastases did not survive long enough to complete the entire treatment cycle. Despite encouraging immunostimulatory and anti-angiogenic effects after intratumoural IL-12 EGT, no clinically relevant outcomes were observed in this study, as persistent tumour regression could not be obtained. On the other hand, the laboratory and US results hold great promise for combinatorial strategies of intratumoural IL-12 EGT with conventional antitumour (immuno)therapies.},
  author       = {Cicchelero E, Laetitia and Denies, Sofie and Haers, Hendrik and Vanderperren, Katrien and Stock, Emmelie and Van Brantegem, Leen and De Rooster, Hilde and Sanders, Niek},
  issn         = {1476-5810},
  journal      = {VETERINARY AND COMPARATIVE ONCOLOGY},
  keyword      = {angiogenesis,contrast-enhanced ultrasound,electroporation,gene transfer,immune stimulation,interleukin 12,spontaneous tumours,CONTRAST-ENHANCED ULTRASOUND,NATURAL-KILLER-CELLS,HUMAN T-CELLS,TUMOR-GROWTH,METASTATIC MELANOMA,GAMMA-INTERFERON,IL-12 RECEPTOR,LYMPH-NODE,IFN-GAMMA,NK-CELLS},
  language     = {eng},
  number       = {4},
  pages        = {1187--1205},
  title        = {Intratumoural interleukin 12 gene therapy stimulates the immune system and decreases angiogenesis in dogs with spontaneous cancer},
  url          = {http://dx.doi.org/10.1111/vco.12255},
  volume       = {15},
  year         = {2017},
}

Chicago
Cicchelero E, Laetitia, Sofie Denies, Hendrik Haers, Katrien Vanderperren, Emmelie Stock, Leen Van Brantegem, Hilde De Rooster, and Niek Sanders. 2017. “Intratumoural Interleukin 12 Gene Therapy Stimulates the Immune System and Decreases Angiogenesis in Dogs with Spontaneous Cancer.” Veterinary and Comparative Oncology 15 (4): 1187–1205.
APA
Cicchelero E, L., Denies, S., Haers, H., Vanderperren, K., Stock, E., Van Brantegem, L., De Rooster, H., et al. (2017). Intratumoural interleukin 12 gene therapy stimulates the immune system and decreases angiogenesis in dogs with spontaneous cancer. VETERINARY AND COMPARATIVE ONCOLOGY, 15(4), 1187–1205.
Vancouver
1.
Cicchelero E L, Denies S, Haers H, Vanderperren K, Stock E, Van Brantegem L, et al. Intratumoural interleukin 12 gene therapy stimulates the immune system and decreases angiogenesis in dogs with spontaneous cancer. VETERINARY AND COMPARATIVE ONCOLOGY. 2017;15(4):1187–205.
MLA
Cicchelero E, Laetitia, Sofie Denies, Hendrik Haers, et al. “Intratumoural Interleukin 12 Gene Therapy Stimulates the Immune System and Decreases Angiogenesis in Dogs with Spontaneous Cancer.” VETERINARY AND COMPARATIVE ONCOLOGY 15.4 (2017): 1187–1205. Print.