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Performance of four modern whole genome amplification methods for copy number variant detection in single cells

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Abstract
Whole genome amplification (WGA) has become an invaluable tool to perform copy number variation (CNV) detection in single, or a limited number of cells. Unfortunately, current WGA methods introduce representation bias that limits the detection of small CNVs. New WGA methods have been introduced that might have the potential to reduce this bias. We compared the performance of PicoPLEX DNA-Seq (Picoseq), DOPlify, REPLI-g and Ampli-1 WGA for aneuploidy screening and copy number analysis using shallow whole genome massively parallel sequencing (MPS), starting from single or a limited number of cells. Although the four WGA methods perform differently, they are all suited for this application.
Keywords
CIRCULATING TUMOR-CELLS, SOMATIC MUTATIONS, CANCER, ANEUPLOIDY, DIAGNOSIS, CATALOG, EMBRYOS

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Please use this url to cite or link to this publication:

MLA
Deleye, Lieselot et al. “Performance of Four Modern Whole Genome Amplification Methods for Copy Number Variant Detection in Single Cells.” SCIENTIFIC REPORTS 7 (2017): n. pag. Print.
APA
Deleye, L., Tilleman, L., Vander Plaetsen, A.-S., Cornelis, S., Deforce, D., & Van Nieuwerburgh, F. (2017). Performance of four modern whole genome amplification methods for copy number variant detection in single cells. SCIENTIFIC REPORTS, 7.
Chicago author-date
Deleye, Lieselot, Laurentijn Tilleman, Ann-Sophie Vander Plaetsen, Senne Cornelis, Dieter Deforce, and Filip Van Nieuwerburgh. 2017. “Performance of Four Modern Whole Genome Amplification Methods for Copy Number Variant Detection in Single Cells.” Scientific Reports 7.
Chicago author-date (all authors)
Deleye, Lieselot, Laurentijn Tilleman, Ann-Sophie Vander Plaetsen, Senne Cornelis, Dieter Deforce, and Filip Van Nieuwerburgh. 2017. “Performance of Four Modern Whole Genome Amplification Methods for Copy Number Variant Detection in Single Cells.” Scientific Reports 7.
Vancouver
1.
Deleye L, Tilleman L, Vander Plaetsen A-S, Cornelis S, Deforce D, Van Nieuwerburgh F. Performance of four modern whole genome amplification methods for copy number variant detection in single cells. SCIENTIFIC REPORTS. 2017;7.
IEEE
[1]
L. Deleye, L. Tilleman, A.-S. Vander Plaetsen, S. Cornelis, D. Deforce, and F. Van Nieuwerburgh, “Performance of four modern whole genome amplification methods for copy number variant detection in single cells,” SCIENTIFIC REPORTS, vol. 7, 2017.
@article{8524377,
  abstract     = {Whole genome amplification (WGA) has become an invaluable tool to perform copy number variation (CNV) detection in single, or a limited number of cells. Unfortunately, current WGA methods introduce representation bias that limits the detection of small CNVs. New WGA methods have been introduced that might have the potential to reduce this bias. We compared the performance of PicoPLEX DNA-Seq (Picoseq), DOPlify, REPLI-g and Ampli-1 WGA for aneuploidy screening and copy number analysis using shallow whole genome massively parallel sequencing (MPS), starting from single or a limited number of cells. Although the four WGA methods perform differently, they are all suited for this application.},
  articleno    = {3422},
  author       = {Deleye, Lieselot and Tilleman, Laurentijn and Vander Plaetsen, Ann-Sophie and Cornelis, Senne and Deforce, Dieter and Van Nieuwerburgh, Filip},
  issn         = {2045-2322},
  journal      = {SCIENTIFIC REPORTS},
  keywords     = {CIRCULATING TUMOR-CELLS,SOMATIC MUTATIONS,CANCER,ANEUPLOIDY,DIAGNOSIS,CATALOG,EMBRYOS},
  language     = {eng},
  pages        = {9},
  title        = {Performance of four modern whole genome amplification methods for copy number variant detection in single cells},
  url          = {http://dx.doi.org/10.1038/s41598-017-03711-y},
  volume       = {7},
  year         = {2017},
}

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