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Direct comparison of distinct naive pluripotent states in human embryonic stem cells

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Abstract
Until recently, human embryonic stem cells (hESCs) were shown to exist in a state of primed pluripotency, while mouse embryonic stem cells (mESCs) display a naive or primed pluripotent state. Here we show the rapid conversion of in-house-derived primed hESCs on mouse embryonic feeder layer (MEF) to a naive state within 5-6 days in naive conversion media (NCM-MEF), 6-10 days in naive human stem cell media (NHSM-MEF) and 14-20 days using the reverse-toggle protocol (RT-MEF). We further observe enhanced unbiased lineage-specific differentiation potential of naive hESCs converted in NCM-MEF, however, all naive hESCs fail to differentiate towards functional cell types. RNA-seq analysis reveals a divergent role of PI3K/AKT/mTORC signalling, specifically of the mTORC2 subunit, in the different naive hESCs. Overall, we demonstrate a direct evaluation of several naive culture conditions performed in the same laboratory, thereby contributing to an unbiased, more in-depth understanding of different naive hESCs.
Keywords
GENE-EXPRESSION-DATA, EARLY MOUSE EMBRYOS, GROUND-STATE, SELF-RENEWAL, SET ENRICHMENT, DIFFERENTIATION, PACKAGE, PRDM14, IDENTIFICATION, TRANSITION

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Chicago
Warrier, Sharat, Margot Van der Jeught, Galbha Duggal, Laurentijn Tilleman, Ella Sutherland, Jasin Taelman, Mina Popovic, et al. 2017. “Direct Comparison of Distinct Naive Pluripotent States in Human Embryonic Stem Cells.” Nature Communications 8.
APA
Warrier, Sharat, Van der Jeught, M., Duggal, G., Tilleman, L., Sutherland, E., Taelman, J., Popovic, M., et al. (2017). Direct comparison of distinct naive pluripotent states in human embryonic stem cells. NATURE COMMUNICATIONS, 8.
Vancouver
1.
Warrier S, Van der Jeught M, Duggal G, Tilleman L, Sutherland E, Taelman J, et al. Direct comparison of distinct naive pluripotent states in human embryonic stem cells. NATURE COMMUNICATIONS. 2017;8.
MLA
Warrier, Sharat, Margot Van der Jeught, Galbha Duggal, et al. “Direct Comparison of Distinct Naive Pluripotent States in Human Embryonic Stem Cells.” NATURE COMMUNICATIONS 8 (2017): n. pag. Print.
@article{8524370,
  abstract     = {Until recently, human embryonic stem cells (hESCs) were shown to exist in a state of primed pluripotency, while mouse embryonic stem cells (mESCs) display a naive or primed pluripotent state. Here we show the rapid conversion of in-house-derived primed hESCs on mouse embryonic feeder layer (MEF) to a naive state within 5-6 days in naive conversion media (NCM-MEF), 6-10 days in naive human stem cell media (NHSM-MEF) and 14-20 days using the reverse-toggle protocol (RT-MEF). We further observe enhanced unbiased lineage-specific differentiation potential of naive hESCs converted in NCM-MEF, however, all naive hESCs fail to differentiate towards functional cell types. RNA-seq analysis reveals a divergent role of PI3K/AKT/mTORC signalling, specifically of the mTORC2 subunit, in the different naive hESCs. Overall, we demonstrate a direct evaluation of several naive culture conditions performed in the same laboratory, thereby contributing to an unbiased, more in-depth understanding of different naive hESCs.},
  articleno    = {15055},
  author       = {Warrier, Sharat and Van der Jeught, Margot and Duggal, Galbha and Tilleman, Laurentijn and Sutherland, Ella and Taelman, Jasin and Popovic, Mina and Lierman, Sylvie and Chuva de Sousa Lopes, Susana Marina and Van Soom, Ann and Peelman, Luc and Van Nieuwerburgh, Filip and De Coninck, Dieter and Menten, Bj{\"o}rn and Mestdagh, Pieter and Van de Sompele, Jeroen and Deforce, Dieter and De Sutter, Petra and Heindryckx, Bj{\"o}rn},
  issn         = {2041-1723},
  journal      = {NATURE COMMUNICATIONS},
  keyword      = {GENE-EXPRESSION-DATA,EARLY MOUSE EMBRYOS,GROUND-STATE,SELF-RENEWAL,SET ENRICHMENT,DIFFERENTIATION,PACKAGE,PRDM14,IDENTIFICATION,TRANSITION},
  language     = {eng},
  pages        = {10},
  title        = {Direct comparison of distinct naive pluripotent states in human embryonic stem cells},
  url          = {http://dx.doi.org/10.1038/ncomms15055},
  volume       = {8},
  year         = {2017},
}

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