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Direct comparison of distinct naive pluripotent states in human embryonic stem cells

Sharat Warrier UGent, Margot Van der Jeught UGent, Galbha Duggal, Laurentijn Tilleman UGent, Ella Sutherland, Jasin Taelman UGent, Mina Popovic UGent, Sylvie Lierman UGent, Susana Marina Chuva de Sousa Lopes UGent, Ann Van Soom UGent, et al. (2017) NATURE COMMUNICATIONS. 8.
abstract
Until recently, human embryonic stem cells (hESCs) were shown to exist in a state of primed pluripotency, while mouse embryonic stem cells (mESCs) display a naive or primed pluripotent state. Here we show the rapid conversion of in-house-derived primed hESCs on mouse embryonic feeder layer (MEF) to a naive state within 5-6 days in naive conversion media (NCM-MEF), 6-10 days in naive human stem cell media (NHSM-MEF) and 14-20 days using the reverse-toggle protocol (RT-MEF). We further observe enhanced unbiased lineage-specific differentiation potential of naive hESCs converted in NCM-MEF, however, all naive hESCs fail to differentiate towards functional cell types. RNA-seq analysis reveals a divergent role of PI3K/AKT/mTORC signalling, specifically of the mTORC2 subunit, in the different naive hESCs. Overall, we demonstrate a direct evaluation of several naive culture conditions performed in the same laboratory, thereby contributing to an unbiased, more in-depth understanding of different naive hESCs.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
GENE-EXPRESSION-DATA, EARLY MOUSE EMBRYOS, GROUND-STATE, SELF-RENEWAL, SET ENRICHMENT, DIFFERENTIATION, PACKAGE, PRDM14, IDENTIFICATION, TRANSITION
journal title
NATURE COMMUNICATIONS
Nat. Commun.
volume
8
article number
15055
pages
10 pages
Web of Science type
Article
Web of Science id
000399983500001
ISSN
2041-1723
DOI
10.1038/ncomms15055
language
English
UGent publication?
yes
classification
A1
copyright statement
Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
id
8524370
handle
http://hdl.handle.net/1854/LU-8524370
date created
2017-06-20 13:42:48
date last changed
2017-09-07 14:09:26
@article{8524370,
  abstract     = {Until recently, human embryonic stem cells (hESCs) were shown to exist in a state of primed pluripotency, while mouse embryonic stem cells (mESCs) display a naive or primed pluripotent state. Here we show the rapid conversion of in-house-derived primed hESCs on mouse embryonic feeder layer (MEF) to a naive state within 5-6 days in naive conversion media (NCM-MEF), 6-10 days in naive human stem cell media (NHSM-MEF) and 14-20 days using the reverse-toggle protocol (RT-MEF). We further observe enhanced unbiased lineage-specific differentiation potential of naive hESCs converted in NCM-MEF, however, all naive hESCs fail to differentiate towards functional cell types. RNA-seq analysis reveals a divergent role of PI3K/AKT/mTORC signalling, specifically of the mTORC2 subunit, in the different naive hESCs. Overall, we demonstrate a direct evaluation of several naive culture conditions performed in the same laboratory, thereby contributing to an unbiased, more in-depth understanding of different naive hESCs.},
  articleno    = {15055},
  author       = {Warrier, Sharat and Van der Jeught, Margot and Duggal, Galbha and Tilleman, Laurentijn and Sutherland, Ella and Taelman, Jasin and Popovic, Mina and Lierman, Sylvie and Chuva de Sousa Lopes, Susana Marina and Van Soom, Ann and Peelman, Luc and Van Nieuwerburgh, Filip and De Coninck, D. I. M. and Menten, Bj{\"o}rn and Mestdagh, Pieter and Van de Sompele, Jeroen and Deforce, Dieter and De Sutter, Petra and Heindryckx, Bj{\"o}rn},
  issn         = {2041-1723},
  journal      = {NATURE COMMUNICATIONS},
  keyword      = {GENE-EXPRESSION-DATA,EARLY MOUSE EMBRYOS,GROUND-STATE,SELF-RENEWAL,SET ENRICHMENT,DIFFERENTIATION,PACKAGE,PRDM14,IDENTIFICATION,TRANSITION},
  language     = {eng},
  pages        = {10},
  title        = {Direct comparison of distinct naive pluripotent states in human embryonic stem cells},
  url          = {http://dx.doi.org/10.1038/ncomms15055},
  volume       = {8},
  year         = {2017},
}

Chicago
Warrier, Sharat, Margot Van der Jeught, Galbha Duggal, Laurentijn Tilleman, Ella Sutherland, Jasin Taelman, Mina Popovic, et al. 2017. “Direct Comparison of Distinct Naive Pluripotent States in Human Embryonic Stem Cells.” Nature Communications 8.
APA
Warrier, Sharat, Van der Jeught, M., Duggal, G., Tilleman, L., Sutherland, E., Taelman, J., Popovic, M., et al. (2017). Direct comparison of distinct naive pluripotent states in human embryonic stem cells. NATURE COMMUNICATIONS, 8.
Vancouver
1.
Warrier S, Van der Jeught M, Duggal G, Tilleman L, Sutherland E, Taelman J, et al. Direct comparison of distinct naive pluripotent states in human embryonic stem cells. NATURE COMMUNICATIONS. 2017;8.
MLA
Warrier, Sharat, Margot Van der Jeught, Galbha Duggal, et al. “Direct Comparison of Distinct Naive Pluripotent States in Human Embryonic Stem Cells.” NATURE COMMUNICATIONS 8 (2017): n. pag. Print.