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The short neuropeptide F modulates olfactory sensitivity of Bactrocera dorsalis upon starvation

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Abstract
The insect short neuropeptide F (sNPF) family has been shown to modulate diverse physiological processes, such as feeding, appetitive olfactory behavior, locomotion, sleep homeostasis and hormone release. In this study, we identified the sNPF (BdsNPF) and its receptor (BdsNPFR) in an important agricultural pest, the oriental fruit fly Bactrocera dorsalis (Hendel). Afterwards, the receptor cDNA was functionally expressed in Chinese hamster ovary cell lines. Activation of BdsNPFR by sNPF peptides caused an increase in intracellular calcium ions, with a 50% effective concentration values at the nanomolar level. As indicated by qPCR, the BdsNPF and BdsNPFR transcripts were mainly detected in the central nervous system and antennae, and they showed significantly starvation-induced expression patterns. Furthermore, we found that the starved flies had an increased electroantennogram response compared to the normally fed flies. However, this enhanced olfactory sensitivity was reversed when we decreased the expression of BdsNPF by double-stranded RNA injection in adults. We concluded that sNPF plays an important role in modulating the olfactory sensitivity of B. dorsalis upon starvation. Our results will facilitate the understanding of the regulation of early olfactory processing in B. dorsalis.
Keywords
Oriental fruit fly, Short neuropeptide F, G-protein coupled-receptor, Olfaction sensing, Starvation, ORIENTAL FRUIT-FLY, DROSOPHILA-MELANOGASTER, DESERT LOCUST, RECEPTOR, BEHAVIOR, MECHANISM, INSECTS, PEPTIDE, COMPLEX, MIDGUT

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Chicago
Jiang, Hong-Bo, Shun-Hua Gui, Li Xu, Yu-Xia Pei, Guy Smagghe, and Jin-Jun Wang. 2017. “The Short Neuropeptide F Modulates Olfactory Sensitivity of Bactrocera Dorsalis Upon Starvation.” Journal of Insect Physiology 99: 78–85.
APA
Jiang, H.-B., Gui, S.-H., Xu, L., Pei, Y.-X., Smagghe, G., & Wang, J.-J. (2017). The short neuropeptide F modulates olfactory sensitivity of Bactrocera dorsalis upon starvation. JOURNAL OF INSECT PHYSIOLOGY, 99, 78–85.
Vancouver
1.
Jiang H-B, Gui S-H, Xu L, Pei Y-X, Smagghe G, Wang J-J. The short neuropeptide F modulates olfactory sensitivity of Bactrocera dorsalis upon starvation. JOURNAL OF INSECT PHYSIOLOGY. 2017;99:78–85.
MLA
Jiang, Hong-Bo, Shun-Hua Gui, Li Xu, et al. “The Short Neuropeptide F Modulates Olfactory Sensitivity of Bactrocera Dorsalis Upon Starvation.” JOURNAL OF INSECT PHYSIOLOGY 99 (2017): 78–85. Print.
@article{8524347,
  abstract     = {The insect short neuropeptide F (sNPF) family has been shown to modulate diverse physiological processes, such as feeding, appetitive olfactory behavior, locomotion, sleep homeostasis and hormone release. In this study, we identified the sNPF (BdsNPF) and its receptor (BdsNPFR) in an important agricultural pest, the oriental fruit fly Bactrocera dorsalis (Hendel). Afterwards, the receptor cDNA was functionally expressed in Chinese hamster ovary cell lines. Activation of BdsNPFR by sNPF peptides caused an increase in intracellular calcium ions, with a 50\% effective concentration values at the nanomolar level. As indicated by qPCR, the BdsNPF and BdsNPFR transcripts were mainly detected in the central nervous system and antennae, and they showed significantly starvation-induced expression patterns. Furthermore, we found that the starved flies had an increased electroantennogram response compared to the normally fed flies. However, this enhanced olfactory sensitivity was reversed when we decreased the expression of BdsNPF by double-stranded RNA injection in adults. We concluded that sNPF plays an important role in modulating the olfactory sensitivity of B. dorsalis upon starvation. Our results will facilitate the understanding of the regulation of early olfactory processing in B. dorsalis.},
  author       = {Jiang, Hong-Bo and Gui, Shun-Hua and Xu, Li and Pei, Yu-Xia and Smagghe, Guy and Wang, Jin-Jun},
  issn         = {0022-1910},
  journal      = {JOURNAL OF INSECT PHYSIOLOGY},
  language     = {eng},
  pages        = {78--85},
  title        = {The short neuropeptide F modulates olfactory sensitivity of Bactrocera dorsalis upon starvation},
  url          = {http://dx.doi.org/10.1016/j.jinsphys.2017.03.012},
  volume       = {99},
  year         = {2017},
}

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