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Infections of virulent and avirulent viruses differentially influenced the expression of dicer-1, ago-1, and microRNAs in Bombus terrestris

Jinzhi Niu (UGent) , Ivan Meeus (UGent) , Dieter De Coninck (UGent) , Dieter Deforce (UGent) , Kayvan Etebari, Sassan Asgari and Guy Smagghe (UGent)
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Abstract
The microRNA (miRNA) pathway is well established to be involved in host-pathogen interactions. As key insect pollinators, bees are suffering from widely spreading viruses, especially honeybees and bumblebees. In order to better understand bee-virus interaction, we comparatively analyzed the involvement of the bumblebee miRNA pathway upon infection by two different viruses. In our setup, an avirulent infection is induced by slow bee paralysis virus (SBPV) and a virulent infection is induced by Israeli acute paralysis virus (IAPV). Our results showed the increased expressions of dicer-1 and ago-1 upon SBPV infection. There were 17 and 12 bumblebee miRNAs differentially expressed upon SBPV and IAPV infections, respectively. These results may indicate the involvement of the host miRNA pathway in bumblebee-virus interaction. However, silencing of dicer-1 did not influence the genome copy number of SBPV. Target prediction for these differentially expressed miRNAs showed their possible involvement in targeting viral genomic RNA and in the regulation of networks in bumblebee. Our study opens a new insight into bee-virus interaction meditated by host miRNAs.
Keywords
INTERFERING RNA PATHWAY, SIGNALING PATHWAY, IMMUNE-RESPONSE, DROSOPHILA, GENE, VAGO, HOMEOSTASIS, BIOGENESIS, PATTERN, INSULIN

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Chicago
Niu, Jinzhi, Ivan Meeus, Dieter De Coninck, Dieter Deforce, Kayvan Etebari, Sassan Asgari, and Guy Smagghe. 2017. “Infections of Virulent and Avirulent Viruses Differentially Influenced the Expression of Dicer-1, Ago-1, and microRNAs in Bombus Terrestris.” Scientific Reports 7.
APA
Niu, J., Meeus, I., De Coninck, D., Deforce, D., Etebari, K., Asgari, S., & Smagghe, G. (2017). Infections of virulent and avirulent viruses differentially influenced the expression of dicer-1, ago-1, and microRNAs in Bombus terrestris. SCIENTIFIC REPORTS, 7.
Vancouver
1.
Niu J, Meeus I, De Coninck D, Deforce D, Etebari K, Asgari S, et al. Infections of virulent and avirulent viruses differentially influenced the expression of dicer-1, ago-1, and microRNAs in Bombus terrestris. SCIENTIFIC REPORTS. 2017;7.
MLA
Niu, Jinzhi et al. “Infections of Virulent and Avirulent Viruses Differentially Influenced the Expression of Dicer-1, Ago-1, and microRNAs in Bombus Terrestris.” SCIENTIFIC REPORTS 7 (2017): n. pag. Print.
@article{8524339,
  abstract     = {The microRNA (miRNA) pathway is well established to be involved in host-pathogen interactions. As key insect pollinators, bees are suffering from widely spreading viruses, especially honeybees and bumblebees. In order to better understand bee-virus interaction, we comparatively analyzed the involvement of the bumblebee miRNA pathway upon infection by two different viruses. In our setup, an avirulent infection is induced by slow bee paralysis virus (SBPV) and a virulent infection is induced by Israeli acute paralysis virus (IAPV). Our results showed the increased expressions of dicer-1 and ago-1 upon SBPV infection. There were 17 and 12 bumblebee miRNAs differentially expressed upon SBPV and IAPV infections, respectively. These results may indicate the involvement of the host miRNA pathway in bumblebee-virus interaction. However, silencing of dicer-1 did not influence the genome copy number of SBPV. Target prediction for these differentially expressed miRNAs showed their possible involvement in targeting viral genomic RNA and in the regulation of networks in bumblebee. Our study opens a new insight into bee-virus interaction meditated by host miRNAs.},
  articleno    = {45620},
  author       = {Niu, Jinzhi and Meeus, Ivan and De Coninck, Dieter and Deforce, Dieter and Etebari, Kayvan and Asgari, Sassan and Smagghe, Guy},
  issn         = {2045-2322},
  journal      = {SCIENTIFIC REPORTS},
  keywords     = {INTERFERING RNA PATHWAY,SIGNALING PATHWAY,IMMUNE-RESPONSE,DROSOPHILA,GENE,VAGO,HOMEOSTASIS,BIOGENESIS,PATTERN,INSULIN},
  language     = {eng},
  pages        = {11},
  title        = {Infections of virulent and avirulent viruses differentially influenced the expression of dicer-1, ago-1, and microRNAs in Bombus terrestris},
  url          = {http://dx.doi.org/10.1038/srep45620},
  volume       = {7},
  year         = {2017},
}

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