Advanced search
1 file | 1.05 MB

Quantification of egg ovalbumin hydrolysate-derived anti-hypertensive peptides in an in vitro model combining luminal digestion with intestinal Caco-2 cell transport

(2017) FOOD RESEARCH INTERNATIONAL. 99(1). p.531-541
Author
Organization
Abstract
Food-derived peptides can impact blood pressure through several mechanisms. However, their fate in. the gastrointestinal tract and bioavailability are difficult to assess because of their fast degradation and challenging analysis in physiologically relevant matrices. The aim of this study was to construct an in vitro bioavailability methodology in which luminal digestion is combined with Caco-2 cell transport. Egg ovalbumin hydrolysate, both in pure form and mixed with a food matrix, was used as a test case. Results indicate that a food matrix protected bioactive peptides from luminal digestion, especially in small intestine. Moreover, the Caco-2 absorption peak was extended over a longer time period (> 60 min) compared to the pure peptide solutions (similar to 15 min) which in total resulted in a 3-12 times higher absorption of the bioactive sequences after 60 min compared to fasted conditions. These results suggest further investigation is warranted towards peptide-based functional foods with improved gastro-intestinal stability and longer-term release in the blood.
Keywords
ACE, Caco-2, Digestion, Egg, LC-MS/MS, SPONTANEOUSLY HYPERTENSIVE-RATS, ENZYME-INHIBITORY PEPTIDES, LONG-TERM INTAKE, BIOACTIVE PEPTIDES, TRANSEPITHELIAL TRANSPORT, WHITE PROTEINS, MONOLAYERS, FOOD, BIOAVAILABILITY, PRODUCTS

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 1.05 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Grootaert, Charlotte, Griet Jacobs, Bea Matthijs, Judit Pitart, Geert Baggerman, Sam Possemiers, Hans Van der Saag, Guy Smagghe, John Van Camp, and Stefan Voorspoels. 2017. “Quantification of Egg Ovalbumin Hydrolysate-derived Anti-hypertensive Peptides in an in Vitro Model Combining Luminal Digestion with Intestinal Caco-2 Cell Transport.” Food Research International 99 (1): 531–541.
APA
Grootaert, C., Jacobs, G., Matthijs, B., Pitart, J., Baggerman, G., Possemiers, S., Van der Saag, H., et al. (2017). Quantification of egg ovalbumin hydrolysate-derived anti-hypertensive peptides in an in vitro model combining luminal digestion with intestinal Caco-2 cell transport. FOOD RESEARCH INTERNATIONAL, 99(1), 531–541.
Vancouver
1.
Grootaert C, Jacobs G, Matthijs B, Pitart J, Baggerman G, Possemiers S, et al. Quantification of egg ovalbumin hydrolysate-derived anti-hypertensive peptides in an in vitro model combining luminal digestion with intestinal Caco-2 cell transport. FOOD RESEARCH INTERNATIONAL. 2017;99(1):531–41.
MLA
Grootaert, Charlotte, Griet Jacobs, Bea Matthijs, et al. “Quantification of Egg Ovalbumin Hydrolysate-derived Anti-hypertensive Peptides in an in Vitro Model Combining Luminal Digestion with Intestinal Caco-2 Cell Transport.” FOOD RESEARCH INTERNATIONAL 99.1 (2017): 531–541. Print.
@article{8524237,
  abstract     = {Food-derived peptides can impact blood pressure through several mechanisms. However, their fate in. the gastrointestinal tract and bioavailability are difficult to assess because of their fast degradation and challenging analysis in physiologically relevant matrices. The aim of this study was to construct an in vitro bioavailability methodology in which luminal digestion is combined with Caco-2 cell transport. Egg ovalbumin hydrolysate, both in pure form and mixed with a food matrix, was used as a test case. Results indicate that a food matrix protected bioactive peptides from luminal digestion, especially in small intestine. Moreover, the Caco-2 absorption peak was extended over a longer time period ({\textrangle} 60 min) compared to the pure peptide solutions (similar to 15 min) which in total resulted in a 3-12 times higher absorption of the bioactive sequences after 60 min compared to fasted conditions. These results suggest further investigation is warranted towards peptide-based functional foods with improved gastro-intestinal stability and longer-term release in the blood.},
  author       = {Grootaert, Charlotte and Jacobs, Griet and Matthijs, Bea and Pitart, Judit and Baggerman, Geert and Possemiers, Sam and Van der Saag, Hans and Smagghe, Guy and Van Camp, John and Voorspoels, Stefan},
  isbn         = {0963-9969},
  journal      = {FOOD RESEARCH INTERNATIONAL},
  keyword      = {ACE,Caco-2,Digestion,Egg,LC-MS/MS,SPONTANEOUSLY HYPERTENSIVE-RATS,ENZYME-INHIBITORY PEPTIDES,LONG-TERM INTAKE,BIOACTIVE PEPTIDES,TRANSEPITHELIAL TRANSPORT,WHITE PROTEINS,MONOLAYERS,FOOD,BIOAVAILABILITY,PRODUCTS},
  language     = {eng},
  number       = {1},
  pages        = {531--541},
  title        = {Quantification of egg ovalbumin hydrolysate-derived anti-hypertensive peptides in an in vitro model combining luminal digestion with intestinal Caco-2 cell transport},
  url          = {http://dx.doi.org/10.1016/j.foodres.2017.06.002},
  volume       = {99},
  year         = {2017},
}

Altmetric
View in Altmetric
Web of Science
Times cited: