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Anode potential influences the structure and function of anodic electrode and electrolyte-associated microbiomes

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Abstract
Three bioelectrochemical systems were operated with set anode potentials of +300 mV, +550 mV and +800 mV vs. Standard Hydrogen Electrode (SHE) to test the hypothesis that anode potential influences microbial diversity and is positively associated with microbial biomass and activity. Bacterial and archaeal diversity was characterized using 16 S rRNA gene amplicon sequencing, and biofilm thickness was measured as a proxy for biomass. Current production and substrate utilization patterns were used as measures of microbial activity and the mid-point potentials of putative terminal oxidases were assessed using cyclic voltammetry. All measurements were performed after 4, 16, 23, 30 and 38 days. Microbial biomass and activity differed significantly between anode potentials and were lower at the highest potential. Anodic electrode and electrolyte associated community composition was also significantly influenced by anode potential. While biofilms at +800 mV were thinner, transferred less charge and oxidized less substrate than those at lower potentials, they were also associated with putative terminal oxidases with higher mid-point potentials and generated more biomass per unit charge. This indicates that microbes at +800 mV were unable to capitalize on the potential for additional energy gain due to a lack of adaptive traits to high potential solid electron acceptors and/or sensitivity to oxidative stress.
Keywords
BIOELECTROCHEMICAL SYSTEM, GEOBACTER-SULFURREDUCENS, FUEL-CELLS, MICROORGANISMS, COMMUNITIES, ELECTRICITY, DYNAMICS, BIOFILMS, GROWTH, ARB

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MLA
Dennis, Paul G, Bernardino Virdis, Inka Vanwonterghem, et al. “Anode Potential Influences the Structure and Function of Anodic Electrode and Electrolyte-associated Microbiomes.” SCIENTIFIC REPORTS 6 (2016): n. pag. Print.
APA
Dennis, P. G., Virdis, B., Vanwonterghem, I., Hassan, A., Hugenholtz, P., Tyson, G. W., & Rabaey, K. (2016). Anode potential influences the structure and function of anodic electrode and electrolyte-associated microbiomes. SCIENTIFIC REPORTS, 6.
Chicago author-date
Dennis, Paul G, Bernardino Virdis, Inka Vanwonterghem, Alif Hassan, Phil Hugenholtz, Gene W Tyson, and Korneel Rabaey. 2016. “Anode Potential Influences the Structure and Function of Anodic Electrode and Electrolyte-associated Microbiomes.” Scientific Reports 6.
Chicago author-date (all authors)
Dennis, Paul G, Bernardino Virdis, Inka Vanwonterghem, Alif Hassan, Phil Hugenholtz, Gene W Tyson, and Korneel Rabaey. 2016. “Anode Potential Influences the Structure and Function of Anodic Electrode and Electrolyte-associated Microbiomes.” Scientific Reports 6.
Vancouver
1.
Dennis PG, Virdis B, Vanwonterghem I, Hassan A, Hugenholtz P, Tyson GW, et al. Anode potential influences the structure and function of anodic electrode and electrolyte-associated microbiomes. SCIENTIFIC REPORTS. 2016;6.
IEEE
[1]
P. G. Dennis et al., “Anode potential influences the structure and function of anodic electrode and electrolyte-associated microbiomes,” SCIENTIFIC REPORTS, vol. 6, 2016.
@article{8523566,
  abstract     = {Three bioelectrochemical systems were operated with set anode potentials of +300 mV, +550 mV and +800 mV vs. Standard Hydrogen Electrode (SHE) to test the hypothesis that anode potential influences microbial diversity and is positively associated with microbial biomass and activity. Bacterial and archaeal diversity was characterized using 16 S rRNA gene amplicon sequencing, and biofilm thickness was measured as a proxy for biomass. Current production and substrate utilization patterns were used as measures of microbial activity and the mid-point potentials of putative terminal oxidases were assessed using cyclic voltammetry. All measurements were performed after 4, 16, 23, 30 and 38 days. Microbial biomass and activity differed significantly between anode potentials and were lower at the highest potential. Anodic electrode and electrolyte associated community composition was also significantly influenced by anode potential. While biofilms at +800 mV were thinner, transferred less charge and oxidized less substrate than those at lower potentials, they were also associated with putative terminal oxidases with higher mid-point potentials and generated more biomass per unit charge. This indicates that microbes at +800 mV were unable to capitalize on the potential for additional energy gain due to a lack of adaptive traits to high potential solid electron acceptors and/or sensitivity to oxidative stress.},
  articleno    = {39114},
  author       = {Dennis, Paul G and Virdis, Bernardino and Vanwonterghem, Inka and Hassan, Alif and Hugenholtz, Phil and Tyson, Gene W and Rabaey, Korneel},
  issn         = {2045-2322},
  journal      = {SCIENTIFIC REPORTS},
  keywords     = {BIOELECTROCHEMICAL SYSTEM,GEOBACTER-SULFURREDUCENS,FUEL-CELLS,MICROORGANISMS,COMMUNITIES,ELECTRICITY,DYNAMICS,BIOFILMS,GROWTH,ARB},
  language     = {eng},
  pages        = {11},
  title        = {Anode potential influences the structure and function of anodic electrode and electrolyte-associated microbiomes},
  url          = {http://dx.doi.org/10.1038/srep39114},
  volume       = {6},
  year         = {2016},
}

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