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Degradation of lignin β-aryl ether units in Arabidopsis thaliana expressing LigD, LigF and LigG from Sphingomonas paucimobilis SYK-6

Ewelina Mnich, Ruben Vanholme UGent, Paula Oyarce Sgro UGent, Sarah Liu, Fachuang Lu, Geert Goeminne UGent, Bodil Jørgensen, Mohammed S Motawie, Wout Boerjan UGent, John Ralph, et al. (2017) PLANT BIOTECHNOLOGY JOURNAL. 15(5). p.581-593
abstract
Lignin is a major polymer in the secondary plant cell wall and composed of hydrophobic interlinked hydroxyphenylpropanoid units. The presence of lignin hampers conversion of plant biomass into biofuels; plants with modified lignin are therefore being investigated for increased digestibility. The bacterium Sphingomonas paucimobilis produces lignin-degrading enzymes including LigD, LigF and LigG involved in cleaving the most abundant lignin interunit linkage, the beta-aryl ether bond. In this study, we expressed the LigD, LigF and LigG (LigDFG) genes in Arabidopsis thaliana to introduce postlignification modifications into the lignin structure. The three enzymes were targeted to the secretory pathway. Phenolic metabolite profiling and 2D HSQC NMR of the transgenic lines showed an increase in oxidized guaiacyl and syringyl units without concomitant increase in oxidized beta-aryl ether units, showing lignin bond cleavage. Saccharification yield increased significantly in transgenic lines expressing LigDFG, showing the applicability of our approach. Additional new information on substrate specificity of the LigDFG enzymes is also provided.
Please use this url to cite or link to this publication:
author
organization
alternative title
Degradation of lignin beta-aryl ether units in Arabidopsis thaliana expressing LigD, LigF and LigG from Sphingomonas paucimobilis SYK-6
year
type
journalArticle (original)
publication status
published
subject
keyword
SP STRAIN SYK-6, LAMBDA GLUTATHIONE TRANSFERASES, SOLUTION-STATE NMR, CELL-WALL, BIOSYNTHESIS, ENZYMES, BIOMASS, PLANTS, ROLES, TRANSFORMATION, biofuel, lignin modification, bacteria, Sphingomonas paucimobilis, Lig, beta-aryl ether, saccharification yield
journal title
PLANT BIOTECHNOLOGY JOURNAL
Plant Biotechnol. J.
volume
15
issue
5
pages
581 - 593
Web of Science type
Article
Web of Science id
000401931600004
ISSN
1467-7644
1467-7652
DOI
10.1111/pbi.12655
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
8523529
handle
http://hdl.handle.net/1854/LU-8523529
date created
2017-06-13 11:13:03
date last changed
2017-08-10 09:59:55
@article{8523529,
  abstract     = {Lignin is a major polymer in the secondary plant cell wall and composed of hydrophobic interlinked hydroxyphenylpropanoid units. The presence of lignin hampers conversion of plant biomass into biofuels; plants with modified lignin are therefore being investigated for increased digestibility. The bacterium Sphingomonas paucimobilis produces lignin-degrading enzymes including LigD, LigF and LigG involved in cleaving the most abundant lignin interunit linkage, the beta-aryl ether bond. In this study, we expressed the LigD, LigF and LigG (LigDFG) genes in Arabidopsis thaliana to introduce postlignification modifications into the lignin structure. The three enzymes were targeted to the secretory pathway. Phenolic metabolite profiling and 2D HSQC NMR of the transgenic lines showed an increase in oxidized guaiacyl and syringyl units without concomitant increase in oxidized beta-aryl ether units, showing lignin bond cleavage. Saccharification yield increased significantly in transgenic lines expressing LigDFG, showing the applicability of our approach. Additional new information on substrate specificity of the LigDFG enzymes is also provided.},
  author       = {Mnich, Ewelina and Vanholme, Ruben and Oyarce Sgro, Paula and Liu, Sarah and Lu, Fachuang and Goeminne, Geert and J{\o}rgensen, Bodil and Motawie, Mohammed S and Boerjan, Wout and Ralph, John and Ulvskov, Peter and M{\o}ller, Birger L and Bjarnholt, Nanna and Harholt, Jesper},
  issn         = {1467-7644},
  journal      = {PLANT BIOTECHNOLOGY JOURNAL},
  keyword      = {SP STRAIN SYK-6,LAMBDA GLUTATHIONE TRANSFERASES,SOLUTION-STATE NMR,CELL-WALL,BIOSYNTHESIS,ENZYMES,BIOMASS,PLANTS,ROLES,TRANSFORMATION,biofuel,lignin modification,bacteria,Sphingomonas paucimobilis,Lig,beta-aryl ether,saccharification yield},
  language     = {eng},
  number       = {5},
  pages        = {581--593},
  title        = {Degradation of lignin \ensuremath{\beta}-aryl ether units in Arabidopsis thaliana expressing LigD, LigF and LigG from Sphingomonas paucimobilis SYK-6},
  url          = {http://dx.doi.org/10.1111/pbi.12655},
  volume       = {15},
  year         = {2017},
}

Chicago
Mnich, Ewelina, Ruben Vanholme, Paula Oyarce Sgro, Sarah Liu, Fachuang Lu, Geert Goeminne, Bodil Jørgensen, et al. 2017. “Degradation of Lignin Β-aryl Ether Units in Arabidopsis Thaliana Expressing LigD, LigF and LigG from Sphingomonas Paucimobilis SYK-6.” Plant Biotechnology Journal 15 (5): 581–593.
APA
Mnich, E., Vanholme, R., Oyarce Sgro, P., Liu, S., Lu, F., Goeminne, G., Jørgensen, B., et al. (2017). Degradation of lignin β-aryl ether units in Arabidopsis thaliana expressing LigD, LigF and LigG from Sphingomonas paucimobilis SYK-6. PLANT BIOTECHNOLOGY JOURNAL, 15(5), 581–593.
Vancouver
1.
Mnich E, Vanholme R, Oyarce Sgro P, Liu S, Lu F, Goeminne G, et al. Degradation of lignin β-aryl ether units in Arabidopsis thaliana expressing LigD, LigF and LigG from Sphingomonas paucimobilis SYK-6. PLANT BIOTECHNOLOGY JOURNAL. 2017;15(5):581–93.
MLA
Mnich, Ewelina, Ruben Vanholme, Paula Oyarce Sgro, et al. “Degradation of Lignin Β-aryl Ether Units in Arabidopsis Thaliana Expressing LigD, LigF and LigG from Sphingomonas Paucimobilis SYK-6.” PLANT BIOTECHNOLOGY JOURNAL 15.5 (2017): 581–593. Print.