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Re-annotation, improved large-scale assembly and establishment of a catalogue of noncoding loci for the genome of the model brown alga Ectocarpus

Alexandre Cormier, Komlan Avia, Lieven Sterck (UGent) , Thomas Derrien, Valentin Wucher, Gwendoline Andres, Misharl Monsoor, Olivier Godfroy, Agnieszka Lipinska, Marie-Mathilde Perrineau, et al.
(2017) NEW PHYTOLOGIST. 214(1). p.219-232
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Abstract
The genome of the filamentous brown alga Ectocarpus was the first to be completely sequenced from within the brown algal group and has served as a key reference genome both for this lineage and for the stramenopiles. We present a complete structural and functional reannotation of the Ectocarpus genome. The large-scale assembly of the Ectocarpus genome was significantly improved and genome-wide gene re-annotation using extensive RNA-seq data improved the structure of 11 108 existing protein-coding genes and added 2030 new loci. A genome-wide analysis of splicing isoforms identified an average of 1.6 transcripts per locus. A large number of previously undescribed noncoding genes were identified and annotated, including 717 loci that produce long noncoding RNAs. Conservation of lncRNAs between Ectocarpus and another brown alga, the kelp Saccharina japonica, suggests that at least a proportion of these loci serve a function. Finally, a large collection of single nucleotide polymorphism-based markers was developed for genetic analyses. These resources are available through an updated and improved genome database. This study significantly improves the utility of the Ectocarpus genome as a high-quality reference for the study of many important aspects of brown algal biology and as a reference for genomic analyses across the stramenopiles.
Keywords
alternative splicing, brown algae, Ectocarpus, genetic markers, genome reannotation, long noncoding RNAs, Saccharina japonica, stramenopile, SMALL NUCLEOLAR RNAS, COMPLEX MULTICELLULARITY, SEQ DATA, SILICULOSUS, REVEALS, EVOLUTION, QUANTIFICATION, TRANSCRIPTOME, GENERATION, EXPRESSION

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Citation

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MLA
Cormier, Alexandre, et al. “Re-Annotation, Improved Large-Scale Assembly and Establishment of a Catalogue of Noncoding Loci for the Genome of the Model Brown Alga Ectocarpus.” NEW PHYTOLOGIST, vol. 214, no. 1, 2017, pp. 219–32, doi:10.1111/nph.14321.
APA
Cormier, A., Avia, K., Sterck, L., Derrien, T., Wucher, V., Andres, G., … Cock, J. M. (2017). Re-annotation, improved large-scale assembly and establishment of a catalogue of noncoding loci for the genome of the model brown alga Ectocarpus. NEW PHYTOLOGIST, 214(1), 219–232. https://doi.org/10.1111/nph.14321
Chicago author-date
Cormier, Alexandre, Komlan Avia, Lieven Sterck, Thomas Derrien, Valentin Wucher, Gwendoline Andres, Misharl Monsoor, et al. 2017. “Re-Annotation, Improved Large-Scale Assembly and Establishment of a Catalogue of Noncoding Loci for the Genome of the Model Brown Alga Ectocarpus.” NEW PHYTOLOGIST 214 (1): 219–32. https://doi.org/10.1111/nph.14321.
Chicago author-date (all authors)
Cormier, Alexandre, Komlan Avia, Lieven Sterck, Thomas Derrien, Valentin Wucher, Gwendoline Andres, Misharl Monsoor, Olivier Godfroy, Agnieszka Lipinska, Marie-Mathilde Perrineau, Yves Van de Peer, Christophe Hitte, Erwan Corre, Susana M Coelho, and J Mark Cock. 2017. “Re-Annotation, Improved Large-Scale Assembly and Establishment of a Catalogue of Noncoding Loci for the Genome of the Model Brown Alga Ectocarpus.” NEW PHYTOLOGIST 214 (1): 219–232. doi:10.1111/nph.14321.
Vancouver
1.
Cormier A, Avia K, Sterck L, Derrien T, Wucher V, Andres G, et al. Re-annotation, improved large-scale assembly and establishment of a catalogue of noncoding loci for the genome of the model brown alga Ectocarpus. NEW PHYTOLOGIST. 2017;214(1):219–32.
IEEE
[1]
A. Cormier et al., “Re-annotation, improved large-scale assembly and establishment of a catalogue of noncoding loci for the genome of the model brown alga Ectocarpus,” NEW PHYTOLOGIST, vol. 214, no. 1, pp. 219–232, 2017.
@article{8523484,
  abstract     = {{The genome of the filamentous brown alga Ectocarpus was the first to be completely sequenced from within the brown algal group and has served as a key reference genome both for this lineage and for the stramenopiles. 
We present a complete structural and functional reannotation of the Ectocarpus genome. 
The large-scale assembly of the Ectocarpus genome was significantly improved and genome-wide gene re-annotation using extensive RNA-seq data improved the structure of 11 108 existing protein-coding genes and added 2030 new loci. A genome-wide analysis of splicing isoforms identified an average of 1.6 transcripts per locus. A large number of previously undescribed noncoding genes were identified and annotated, including 717 loci that produce long noncoding RNAs. Conservation of lncRNAs between Ectocarpus and another brown alga, the kelp Saccharina japonica, suggests that at least a proportion of these loci serve a function. Finally, a large collection of single nucleotide polymorphism-based markers was developed for genetic analyses. These resources are available through an updated and improved genome database. 
This study significantly improves the utility of the Ectocarpus genome as a high-quality reference for the study of many important aspects of brown algal biology and as a reference for genomic analyses across the stramenopiles.}},
  author       = {{Cormier, Alexandre and Avia, Komlan and Sterck, Lieven and Derrien, Thomas and Wucher, Valentin and Andres, Gwendoline and Monsoor, Misharl and Godfroy, Olivier and Lipinska, Agnieszka and Perrineau, Marie-Mathilde and Van de Peer, Yves and Hitte, Christophe and Corre, Erwan and Coelho, Susana M and Cock, J Mark}},
  issn         = {{0028-646X}},
  journal      = {{NEW PHYTOLOGIST}},
  keywords     = {{alternative splicing,brown algae,Ectocarpus,genetic markers,genome reannotation,long noncoding RNAs,Saccharina japonica,stramenopile,SMALL NUCLEOLAR RNAS,COMPLEX MULTICELLULARITY,SEQ DATA,SILICULOSUS,REVEALS,EVOLUTION,QUANTIFICATION,TRANSCRIPTOME,GENERATION,EXPRESSION}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{219--232}},
  title        = {{Re-annotation, improved large-scale assembly and establishment of a catalogue of noncoding loci for the genome of the model brown alga Ectocarpus}},
  url          = {{http://dx.doi.org/10.1111/nph.14321}},
  volume       = {{214}},
  year         = {{2017}},
}
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