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The zinc finger transcription factor PW1/PEG3 restrains murine beta cell cycling

(2016) DIABETOLOGIA. 59(7). p.1474-1479
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Abstract
Aims/hypothesis: Pw1 or paternally-expressed gene 3 (Peg3) encodes a zinc finger transcription factor that is widely expressed during mouse embryonic development and later restricted to multiple somatic stem cell lineages in the adult. The aim of the present study was to define Pw1 expression in the embryonic and adult pancreas and investigate its role in the beta cell cycle in Pw1 wild-type and mutant mice. Methods: We analysed PW1 expression by immunohistochemistry in pancreas of nonpregant and pregnant mice and following injury by partial duct ligation. Its role in the beta cell cycle was studied in vivo using a novel conditional knockout mouse and in vitro by lentivirus-mediated gene knockdown. Results: We showed that PW1 is expressed in early pancreatic progenitors at E9.5 but becomes progressively restricted to fully differentiated beta cells as they become established after birth and withdraw from the cell cycle. Notably, PW1 expression declines when beta cells are induced to proliferate and loss of PW1 function activates the beta cell cycle. Conclusions/interpretation: These results indicate that PW1 is a co-regulator of the beta cell cycle and can thus be considered a novel therapeutic target in diabetes.
Keywords
Beta cell, Pancreas development, Peg3, Proliferation, Pw1, ADULT-MOUSE PANCREAS, IMPRINTED GENE, PEG3, LINEAGES, PROLIFERATION, PROGENITORS, EXPRESSION, MEDIATOR, DEATH, LINES

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Chicago
Sojoodi, Mozhdeh, Leslie Stradiot, Karo Tanaka, Yves Heremans, Gunter Leuckx, Vanessa Besson, Willem Staels, et al. 2016. “The Zinc Finger Transcription Factor PW1/PEG3 Restrains Murine Beta Cell Cycling.” Diabetologia 59 (7): 1474–1479.
APA
Sojoodi, M., Stradiot, L., Tanaka, K., Heremans, Y., Leuckx, G., Besson, V., Staels, W., et al. (2016). The zinc finger transcription factor PW1/PEG3 restrains murine beta cell cycling. DIABETOLOGIA, 59(7), 1474–1479.
Vancouver
1.
Sojoodi M, Stradiot L, Tanaka K, Heremans Y, Leuckx G, Besson V, et al. The zinc finger transcription factor PW1/PEG3 restrains murine beta cell cycling. DIABETOLOGIA. 2016;59(7):1474–9.
MLA
Sojoodi, Mozhdeh, Leslie Stradiot, Karo Tanaka, et al. “The Zinc Finger Transcription Factor PW1/PEG3 Restrains Murine Beta Cell Cycling.” DIABETOLOGIA 59.7 (2016): 1474–1479. Print.
@article{8523114,
  abstract     = {Aims/hypothesis: Pw1 or paternally-expressed gene 3 (Peg3) encodes a zinc finger transcription factor that is widely expressed during mouse embryonic development and later restricted to multiple somatic stem cell lineages in the adult. The aim of the present study was to define Pw1 expression in the embryonic and adult pancreas and investigate its role in the beta cell cycle in Pw1 wild-type and mutant mice. 
Methods: We analysed PW1 expression by immunohistochemistry in pancreas of nonpregant and pregnant mice and following injury by partial duct ligation. Its role in the beta cell cycle was studied in vivo using a novel conditional knockout mouse and in vitro by lentivirus-mediated gene knockdown. 
Results: We showed that PW1 is expressed in early pancreatic progenitors at E9.5 but becomes progressively restricted to fully differentiated beta cells as they become established after birth and withdraw from the cell cycle. Notably, PW1 expression declines when beta cells are induced to proliferate and loss of PW1 function activates the beta cell cycle. 
Conclusions/interpretation: These results indicate that PW1 is a co-regulator of the beta cell cycle and can thus be considered a novel therapeutic target in diabetes.},
  author       = {Sojoodi, Mozhdeh and Stradiot, Leslie and Tanaka, Karo and Heremans, Yves and Leuckx, Gunter and Besson, Vanessa and Staels, Willem and Van de Casteele, Mark and Marazzi, Giovanna and Sassoon, David and Heimberg, Harry and Bonfanti, Paola},
  issn         = {0012-186X},
  journal      = {DIABETOLOGIA},
  keyword      = {Beta cell,Pancreas development,Peg3,Proliferation,Pw1,ADULT-MOUSE PANCREAS,IMPRINTED GENE,PEG3,LINEAGES,PROLIFERATION,PROGENITORS,EXPRESSION,MEDIATOR,DEATH,LINES},
  language     = {eng},
  number       = {7},
  pages        = {1474--1479},
  title        = {The zinc finger transcription factor PW1/PEG3 restrains murine beta cell cycling},
  url          = {http://dx.doi.org/10.1007/s00125-016-3954-z},
  volume       = {59},
  year         = {2016},
}

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